TY  - JOUR
A1  - Junemann, Alexander
A1  - Winterhoff, Moritz
A1  - Nordholz, Benjamin
A1  - Rottner, Klemens
A1  - Eichinger, Ludwig
A1  - Gräf, Ralph
A1  - Faix, Jan
T1  - ForC lacks canonical formin activity but bundles actin filaments and is required for multicellular development of Dictyostelium cells
JF  - European journal of cell biology
N2  - Diaphanous-related formins (DRFs) drive the nucleation and elongation of linear actin filaments downstream of Rho GTPase signalling pathways. Dictyostelium formin C (ForC) resembles a DRF, except that it lacks a genuine formin homology domain 1 (FH1), raising the questions whether or not ForC can nucleate and elongate actin filaments. We found that a recombinant ForC-FH2 fragment does not nucleate actin polymerization, but moderately decreases the rate of spontaneous actin assembly and disassembly, although the barbed-end elongation rate in the presence of the formin was not markedly changed. However, the protein bound to and crosslinked actin filaments into loose bundles of mixed polarity. Furthermore, ForC is an important regulator of morphogenesis since ForC-null cells are severely impaired in development resulting in the formation of aberrant fruiting bodies. Immunoblotting revealed that ForC is absent during growth, but becomes detectable at the onset of early aggregation when cells chemotactically stream together to form a multicellular organism, and peaks around the culmination stage. Fluorescence microscopy of cells ectopically expressing a GFP-tagged, N-terminal ForC fragment showed its prominent accumulation in the leading edge, suggesting that ForC may play a role in cell migration. In agreement with its expression profile, no defects were observed in random migration of vegetative mutant cells. Notably, chemotaxis of starved cells towards a source of cAMP was severely impaired as opposed to control. This was, however, largely due to a marked developmental delay of the mutant, as evidenced by the expression profile of the early developmental marker csA. In line with this, chemotaxis was almost restored to wild type levels after prolonged starvation. Finally, we observed a complete failure of phototaxis due to abolished slug formation and a massive reduction of spores consistent with forC promoter-driven expression of beta-galactosidase in prespore cells. Together, these findings demonstrate ForC to be critically involved in signalling of the cytoskeleton during various stages of development.
KW  - Actin bundles
KW  - Cell migration
KW  - Chemotaxis
KW  - Development
KW  - Dictyostelium
KW  - Formin
KW  - Morphogenesis
KW  - Phototaxis
KW  - Spore formation
Y1  - 2013
U6  - https://doi.org/10.1016/j.ejcb.2013.07.001
SN  - 0171-9335
VL  - 92
IS  - 6-7
SP  - 201
EP  - 212
PB  - Elsevier
CY  - Jena
ER  - 
TY  - BOOK
A1  - Mientus, Lukas
A1  - Klempin, Christiane
A1  - Nowak, Anna
A1  - Wyss, Corinne
A1  - Aufschnaiter, Claudia von
A1  - Faix, Ann-Christin
A1  - te Poel, Kathrin
A1  - Wahbe, Nadia
A1  - Pieper, Martin
A1  - Höller, Katharina
A1  - Kallenbach, Lea
A1  - Förster, Magdalena
A1  - Redecker, Anke
A1  - Dick, Mirjam
A1  - Holle, Jörg
A1  - Schneider, Edina
A1  - Rehfeldt, Daniel
A1  - Brauns, Sarah
A1  - Abels, Simone
A1  - Ferencik-Lehmkuhl, Daria
A1  - Fränkel, Silvia
A1  - Frohn, Julia
A1  - Liebsch, Ann-Catherine
A1  - Pech, Detlef
A1  - Schreier, Pascal
A1  - Jessen, Moiken
A1  - Großmann, Uta
A1  - Skintey, Lesya
A1  - Voerkel, Paul
A1  - Vaz Ferreira, Mergenfel A.
A1  - Zimmermann, Jan-Simon
A1  - Buddeberg, Magdalena
A1  - Henke, Vanessa
A1  - Hornberg, Sabine
A1  - Völschow, Yvette
A1  - Warrelmann, Julia-Nadine
A1  - Malek, Jennifer
A1  - Tinnefeld, Anja
A1  - Schmidt, Peggy
A1  - Bauer, Tobias
A1  - Jänisch, Christopher
A1  - Spitzer, Lisa
A1  - Franken, Nadine
A1  - Degeling, Maria
A1  - Preisfeld, Angelika
A1  - Meier, Jana
A1  - Küth, Simon
A1  - Scholl, Daniel
A1  - Vogelsang, Christoph
A1  - Watson, Christina
A1  - Weißbach, Anna
A1  - Kulgemeyer, Christoph
A1  - Oetken, Mandy
A1  - Gorski, Sebastian
A1  - Kubsch, Marcus
A1  - Sorge, Stefan
A1  - Wulff, Peter
A1  - Fellenz, Carolin D.
A1  - Schnell, Susanne
A1  - Larisch, Cathleen
A1  - Kaiser, Franz
A1  - Knott, Christina
A1  - Reimer, Stefanie
A1  - Stegmüller, Nathalie
A1  - Boukrayâa Trabelsi, Kathrin
A1  - Schißlbauer, Franziska
A1  - Lemberger, Lukas
A1  - Barth, Ulrike
A1  - Wiehl, Angelika
A1  - Rogge, Tim
A1  - Böhnke, Anja
A1  - Dietz, Dennis
A1  - Großmann, Leroy
A1  - Wienmeister, Annett
A1  - Zoppke, Till
A1  - Jiang, Lisa
A1  - Grünbauer, Stephanie
A1  - Ostersehlt, Dörte
A1  - Peukert, Sophia
A1  - Schäfer, Christoph
A1  - Löbig, Anna
A1  - Bröll, Leena
A1  - Brandt, Birgit
A1  - Breuer, Meike
A1  - Dausend, Henriette
A1  - Krelle, Michael
A1  - Andersen, Gesine
A1  - Falke, Sascha
A1  - Kindermann-Güzel, Kristin
A1  - Körner, Katrina
A1  - Lottermoser, Lisa-Marie
A1  - Pügner, Kati
A1  - Sonnenburg, Nadine
A1  - Akarsu, Selim
A1  - Rechl, Friederike
A1  - Gadinger, Laureen
A1  - Heinze, Lena
A1  - Wittmann, Eveline
A1  - Franke, Manuela
A1  - Lachmund, Anne-Marie
A1  - Böttger, Julia
A1  - Hannover, Bettina
A1  - Behrendt, Renata
A1  - Conty, Valentina
A1  - Grundmann, Stephanie
A1  - Ghassemi, Novid
A1  - Opitz, Ben
A1  - Brämer, Martin
A1  - Gasparjan, David
A1  - Sambanis, Michaela
A1  - Köster, Hilde
A1  - Lücke, Martin
A1  - Nordmeier, Volkhard
A1  - Schaal, Sonja
A1  - Haberbosch, Maximilian
A1  - Meissner, Maren
A1  - Schaal, Steffen
A1  - Brüchner, Melanie
A1  - Riehle, Tamara
A1  - Leopold, Bengta Marie
A1  - Gerlach, Susanne
A1  - Rau-Patschke, Sarah
A1  - Skorsetz, Nina
A1  - Weber, Nadine
A1  - Damköhler, Jens
A1  - Elsholz, Markus
A1  - Trefzger, Thomas
A1  - Lewek, Tobias
A1  - Borowski, Andreas
ED  - Mientus, Lukas
ED  - Klempin, Christiane
ED  - Nowak, Anna
T1  - Reflexion in der Lehrkräftebildung
BT  - Empirisch – Phasenübergreifend – Interdisziplinär
T3  - Potsdamer Beiträge für Lehrkräftebildung und Bildungsforschung
N2  - Reflexion ist eine Schlüsselkategorie für die professionelle Entwicklung von Lehrkräften, welche als Ausbildungsziel in den Bildungsstandards für die Lehrkräftebildung verankert ist. Eine Verstetigung universitär geprägter Forschung und Modellierung in der praxisnahen Anwendung im schulischen Kontext bietet Potentiale nachhaltiger Professionalisierung. Die Stärkung reflexionsbezogener Kompetenzen durch Empirie und Anwendung scheint eine phasenübergreifende Herausforderung der Lehrkräftebildung zu sein, die es zu bewältigen gilt. Ziele des Tagungsbandes Reflexion in der Lehrkräftebildung sind eine theoretische Schärfung des Konzeptes „Reflexive Professionalisierung“ und der Austausch über Fragen der Einbettung wirksamer reflexionsbezogener Lerngelegenheiten in die Lehrkräftebildung. Forschende und Lehrende der‚ drei Phasen (Studium, Referendariat sowie Fort- und Weiterbildung) der Lehrkräftebildung stellen Lehrkonzepte und Forschungsprojekte zum Thema Reflexion in der Lehrkräftebildung vor und diskutieren diese. Gemeinsam mit Teilnehmenden aller Phasen und von verschiedenen Standorten der Lehrkräftebildung werden zukünftige Herausforderungen identifiziert und Lösungsansätze herausgearbeitet.
T3  - Potsdamer Beiträge zur Lehrkräftebildung und Bildungsforschung - 4 
KW  - Reflexion
KW  - Lehrkräftebildung
KW  - Reflexionskompetenz
KW  - Reflexivität
KW  - Feedback
KW  - Reflection
KW  - Teacher Education
KW  - Reflection Skills
KW  - Reflexivity
KW  - Feedback
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-591717
SN  - 978-3-86956-566-8
SN  - 2626-3556
SN  - 2626-4722
IS  - 4
PB  - Universitätsverlag Potsdam
CY  - Potsdam
ER  -