TY  - JOUR
A1  - Liu, Qinsong
A1  - Vain, Thomas
A1  - Viotti, Corrado
A1  - Doyle, Siamsa M.
A1  - Tarkowska, Danuse
A1  - Novak, Ondrej
A1  - Zipfel, Cyril
A1  - Sitbon, Folke
A1  - Robert, Stephanie
A1  - Hofius, Daniel
T1  - Vacuole integrity maintained by DUF300 proteins is required for brassinosteroid signaling regulation
JF  - Molecular plant
N2  - Brassinosteroid (BR) hormone signaling controls multiple processes during plant growth and development and is initiated at the plasma membrane through the receptor kinase BRASSINOSTEROID INSENSITIVE1 (BRI1) together with co-receptors such as BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1). BRI1 abundance is regulated by endosomal recycling and vacuolar targeting, but the role of vacuole-related proteins in BR receptor dynamics and BR responses remains elusive. Here, we show that the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 (LAZ1) and LAZ1 HOMOLOG1 (LAZ1H1), causes vacuole morphology defects, growth inhibition, and constitutive activation of BR signaling. Intriguingly, tonoplast accumulation of BAK1 was substantially increased and appeared causally linked to enhanced BRI1 trafficking and degradation in laz1 laz1h1 plants. Since unrelated vacuole mutants exhibited normal BR responses, our findings indicate that DUF300 proteins play distinct roles in the regulation of BR signaling by maintaining vacuole integrity required to balance subcellular BAK1 pools and BR receptor distribution.
KW  - brassinosteroid signaling
KW  - vacuole integrity
KW  - DUF300 proteins
KW  - tonoplast
KW  - Arabidopsis
Y1  - 2018
U6  - https://doi.org/10.1016/j.molp.2017.12.015
SN  - 1674-2052
SN  - 1752-9867
VL  - 11
IS  - 4
SP  - 553
EP  - 567
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Poxson, David J.
A1  - Karady, Michal
A1  - Gabrielsson, Roger
A1  - Alkattan, Aziz Y.
A1  - Gustavsson, Anna
A1  - Doyle, Siamsa M.
A1  - Robert, Stephanie
A1  - Ljung, Karin
A1  - Grebe, Markus
A1  - Simon, Daniel T.
A1  - Berggren, Magnus
T1  - Regulating plant physiology with organic electronics
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - The organic electronic ion pump (OEIP) provides flow-free and accurate delivery of small signaling compounds at high spatio-temporal resolution. To date, the application of OEIPs has been limited to delivery of nonaromatic molecules to mammalian systems, particularly for neuroscience applications. However, many long-standing questions in plant biology remain unanswered due to a lack of technology that precisely delivers plant hormones, based on cyclic alkanes or aromatic structures, to regulate plant physiology. Here, we report the employment of OEIPs for the delivery of the plant hormone auxin to induce differential concentration gradients and modulate plant physiology. We fabricated OEIP devices based on a synthesized dendritic polyelectrolyte that enables electrophoretic transport of aromatic substances. Delivery of auxin to transgenic Arabidopsis thaliana seedlings in vivo was monitored in real time via dynamic fluorescent auxin-response reporters and induced physiological responses in roots. Our results provide a starting point for technologies enabling direct, rapid, and dynamic electronic interaction with the biochemical regulation systems of plants.
KW  - auxin
KW  - Arabidopsis thaliana
KW  - dendritic polymer
KW  - bioelectronics
KW  - polyelectrolyte
Y1  - 2017
U6  - https://doi.org/10.1073/pnas.1617758114
SN  - 0027-8424
VL  - 114
SP  - 4597
EP  - 4602
PB  - National Acad. of Sciences
CY  - Washington
ER  -