TY  - JOUR
A1  - Mak, Wing Cheung
A1  - Cheung, Kwan Yee
A1  - Trau, Dieter
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
A1  - Renneberg, Reinhard
T1  - Electrochemical bioassay utilizing encapsulated electrochemical active microcrystal biolabels
N2  - A new approach to perform electrochemical immunoassay based on the utilization of encapsulated microcrystal was developed. The microcrystal labels create a "supernova effect" upon exposure to a desired releasing agent. The microcrystal cores dissolve, and large amounts of signal-generating molecules diffuse across the capsule wall into the outer environment. Layer-by-Layer (LbL) technology was employed for the encapsulation of electrochemical signal- generating microcrystals (ferrocene microcrystals). The encapsulated microcrystals were conjugated with antibody molecules through the adsorption process. The biofunctionalized microcrystals were utilized as a probe for immunoassays. The microcrystal-based label system provided a high-signal molecule to antibody (SIP) ratio of 10(4)-10(5). Microcrystal biolabels with different antibody surface coverage (1.60-5.05 mg m(-2)) were subjected to a solid-phase immunoassay for the detection of mouse immunoglobulin G (M-IgG) molecules. The microcrystal-based immunoassay for the detection of M-IgG performed with microcrystals having antibody surface coverage of 5.05 mg m(-2) showed a sensitivity of 3.93 nA  g(- 1) L-1 with a detection limit of 2.82  g L-1
Y1  - 2005
SN  - 0003-2700
ER  - 
TY  - JOUR
A1  - Brennecke, Johannes
A1  - Ochs, Christopher J.
A1  - Boudhar, Aicha
A1  - Reux, Bastien
A1  - Subramanian, Gomathy Sandhya
A1  - Lear, Martin J.
A1  - Trau, Dieter
A1  - Hobley, Jonathan
T1  - Design, preparation and assessment of surface-immobilised tetraphenylethenes for biosensing applications
JF  - Applied surface science : a journal devoted to applied physics and chemistry of surfaces and interfaces
N2  - Tetraphenylethene (TPE) shows a significant increase of fluorescence intensity when the rotational freedom of its phenyl groups is restricted. This special property allows the use of TPE in sensor applications, which have been previously described for the liquid phase only. However, some applications utilising arrays require the immobilisation of TPE dyes on solid surfaces. In this work, we synthesised and investigated the fluorescence behaviour of TPE derivatives on silica particles and quartz slides and suggest ways to employ the dye's properties in solid phase biosensor applications. 2014 Published by Elsevier B.V.
KW  - Tetraphenylethene Bioassay Fluorescent dye
KW  - Microparticles Reagentless assay
Y1  - 2014
U6  - https://doi.org/10.1016/j.apsusc.2014.04.061
SN  - 0169-4332
SN  - 1873-5584
VL  - 307
SP  - 475
EP  - 481
PB  - Elsevier
CY  - Amsterdam
ER  -