TY  - JOUR
A1  - Kedracki, Dawid
A1  - Chekini, Mahshid
A1  - Maroni, Plinio
A1  - Schlaad, Helmut
A1  - Nardin, Corinne
T1  - Synthesis and Self-Assembly of a DNA Molecular Brush
JF  - Biomacromolecules : an interdisciplinary journal focused at the interface of polymer science and the biological sciences
N2  - We report herein on the polymer-crystallization-assisted thiol-ene photosynthesis of an amphiphilic comb/graft DNA copolymer, or molecular brush, composed of a hydrophobic poly(2-oxazoline) backbone and hydrophilic short single-stranded nucleic acid grafts. Coupling efficiencies are above 60% and thus higher as compared with the straight solid-phase-supported synthesis of amphiphilic DNA block copolymers. The DNA molecular brushes self-assemble into sub-micron-sized spherical structures in water as evidenced by light scattering as well as atomic force and electron microscopy imaging. The nucleotide sequences remain functional, as assessed by UV and fluorescence spectroscopy subsequent to isoindol synthesis at the surface of the structures. The determination of a vesicular morphology is supported by encapsulation and subsequent spectroscopy monitoring of the release of a water-soluble dye and spectroscopic quantification of the hybridization efficiency (30% in average) of the functional nucleic acid strands engaged in structure formation: about one-half of the nucleotide sequences are available for hybridization, whereas the other half are hindered within the self-assembled structure. Because speciation between complementary and non complementary sequences in the medium could be ascertained by confocal laser scanning microscopy, the stable self-assembled molecular brushes demonstrate the potential for sensing applications.
Y1  - 2014
U6  - https://doi.org/10.1021/bm5008713
SN  - 1525-7797
SN  - 1526-4602
VL  - 15
IS  - 9
SP  - 3375
EP  - 3382
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Kedracki, Dawid
A1  - Filippov, Sergey K.
A1  - Gour, Nidhi
A1  - Schlaad, Helmut
A1  - Nardin, Corinne
T1  - Formation of DNA-Copolymer Fibrils Through an Amyloid-Like Nucleation Polymerization Mechanism
JF  - Macromolecular rapid communications
N2  - Conjugation of a hydrophobic poly(2-oxazoline) bearing tertiary amide groups along its backbone with a short single stranded nucleotide sequence results in an amphiphilic comb/graft copolymer, which organizes in fibrils upon direct dissolution in water. Supported by circular dichroism, atomic force microscopy, transmission electron microscopy, and scattering data, fibrils are formed through inter- and intramolecular hydrogen bonding between hydrogen accepting amide groups along the polymer backbone and hydrogen donating nucleic acid grafts leading to the formation of hollow tubes.
KW  - DNA copolymers
KW  - fibers
KW  - hydrogen bonding
KW  - nucleation polymerization
KW  - self-assembly
Y1  - 2015
U6  - https://doi.org/10.1002/marc.201400728
SN  - 1022-1336
SN  - 1521-3927
VL  - 36
IS  - 8
SP  - 768
EP  - 773
PB  - Wiley-VCH
CY  - Weinheim
ER  -