TY  - JOUR
A1  - Wehe, Christoph A.
A1  - Pieper, Imke
A1  - Holtkamp, Michael
A1  - Thyssen, Georgina M.
A1  - Sperling, Michael
A1  - Schwerdtle, Tanja
A1  - Karst, Uwe
T1  - On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes
JF  - Analytical & bioanalytical chemistry
N2  - In order to reveal the time-depending mercury species uptake by human astrocytes, a novel approach for total mercury analysis is presented, which uses an accelerated sample introduction system combined on-line with an inductively coupled plasma mass spectrometer equipped with a collision/reaction cell. Human astrocyte samples were incubated with inorganic mercury (HgCl2), methylmercury chloride (MeHgCl), and thimerosal. After 1-h incubation with Hg2+, cellular concentrations of 3 mu M were obtained, whereas for organic species, concentrations of 14-18 mu M could be found. After 24 h, a cellular accumulation factor of 0.3 was observed for the cells incubated with Hg2+, whereas the organic species both showed values of about 5. Due to the obtained steady-state signals, reliable results with relative standard deviations of well below 5 % and limits of detection in the concentration range of 1 ng L-1 were obtained using external calibration and species-unspecific isotope dilution analysis approaches. The results were further validated using atomic fluorescence spectrometry.
KW  - Mercury
KW  - Thimerosal
KW  - Astrocytes
KW  - ICP-MS
KW  - Isotope dilution analysis
KW  - Automation
Y1  - 2014
U6  - https://doi.org/10.1007/s00216-013-7608-4
SN  - 1618-2642
SN  - 1618-2650
VL  - 406
IS  - 7
SP  - 1909
EP  - 1916
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Banerji, Amitabh
A1  - Thyssen, Christoph
A1  - Pampel, Barbara
A1  - Huwer, Johannes
T1  - Naturwissenschaftsunterricht und Informatik
BT  - bringt zusammen, was zusammen gehört?!
BT  - bringing together, what belongs together?!
JF  - Chemie konkret : CHEMKON ; Forum für Unterricht und Didaktik
N2  - Computer literacy plays an increasingly important role in the education of 21st-century society. For chemistry' education. this results in two aspects: On the one hand. informatics education concepts can help to promote chemistry- and science-specific ways of thinking and working. On the other hand, chemistry education can contribute to information education. This paper addresses both aspects and tries to point out the Mutual benefits of informatics education and science education with regards to chemistry lessons.
N2  - Informatische Bildung spielt eine immer zentralere Rolle in der Bildung einer Gesellschaft des 21. Jahrhunderts. Für den Chemieunterricht ergeben sich daraus zwei Aspekte: Einerseits können Konzepte der informatischen Bildung dabei helfen, chemie- und naturwissenschaftsspezifische Denk- und Arbeitsweisen zu fördern. Andererseits kann der Chemieunterricht einen Beitrag für die informatische Bildung leisten. Dieser Artikel geht auf beide Aspekte ein und versucht die gegenseitigen Vorteile der informatischen Bildung und der naturwissenschaftlichen Bildung im Chemieunterricht darzustellen.
T2  - Science education and computer literacy
KW  - computer literacy
KW  - scientific literacy
KW  - informatische Bildung
KW  - naturwissenschaftliche Bildung
Y1  - 2021
U6  - https://doi.org/10.1002/ckon.202100008
SN  - 0944-5846
SN  - 1521-3730
VL  - 28
IS  - 6
SP  - 263
EP  - 265
PB  - Wiley-VCH
CY  - Weinheim
ER  -