TY  - JOUR
A1  - Schröder, Christiane
A1  - Pokorny, Ina
A1  - Dolgener, Nicola
A1  - Herden, Christoph
A1  - Drews, Hauke
A1  - Tiedemann, Ralph
T1  - Allochthonous individuals in managed populations of the fire-bellied toad Bombina bombina genetic detection and conservation implications
JF  - Limnologica : ecology and management of inland waters
N2  - The ongoing global amphibian decline calls for an increase of habitat and population management efforts. Pond restoration and construction is more and more accompanied by breeding and translocation programs. However, the appropriateness of translocations as a tool for conservation has been widely debated, as it can cause biodiversity loss through genetic homogenization and can disrupt local adaptation, eventually leading to outbreeding depression. In this study, we investigated the genetic structure of two translocated populations of the critically endangered fire-bellied toad Bombina bombina at its north western distribution edge using supposedly neutral genetic markers (variation in the mitochondrial control region and microsatellites) as well as a marker under selection (major histocompatibility complex (MHC) genes). While one of the newly established populations showed the typical genetic composition of surrounding populations, the other was extremely diverged without clear affinity to its putative source. In this population we detected a profound impact of allochthonous individuals: 100% of the analyzed individuals exhibited a highly divergent mitochondrial haplotype which was otherwise found in Austria. 83% of them were also assigned to Austria by the analysis of microsatellites. Interestingly, for the adaptive marker (MHC) local alleles were predominant in this population, while only very few alleles were shared with the Austrian population. Probably Mendelian inheritance has reshuffled genotypes such that adaptive local alleles are maintained (here, MHC), while presumably neutral allochthonous alleles dominate at other loci. The release of allochthonous individuals generally increased the genetic variability of the affected population without wiping out locally adaptive genotypes. Thus, outbreeding depression might be less apparent than sometimes thought and natural selection appears strong enough to maintain locally adaptive alleles, at least in functionally important immune system genes.
KW  - Bombina bombina
KW  - Microsatellites
KW  - MHC
KW  - Mitochondrial DNA
KW  - Population management
KW  - Translocation
Y1  - 2012
U6  - https://doi.org/10.1016/j.limno.2012.08.008
SN  - 0075-9511
VL  - 42
IS  - 4
SP  - 291
EP  - 298
PB  - Elsevier
CY  - Jena
ER  - 
TY  - JOUR
A1  - Schmidt, Sabrina
A1  - Saxenhofer, Moritz
A1  - Drewes, Stephan
A1  - Schlegel, Mathias
A1  - Wanka, Konrad M.
A1  - Frank, Raphael
A1  - Klimpel, Sven
A1  - von Blanckenhagen, Felix
A1  - Maaz, Denny
A1  - Herden, Christiane
A1  - Freise, Jona
A1  - Wolf, Ronny
A1  - Stubbe, Michael
A1  - Borkenhagen, Peter
A1  - Ansorge, Hermann
A1  - Eccard, Jana
A1  - Lang, Johannes
A1  - Jourdain, Elsa
A1  - Jacob, Jens
A1  - Marianneau, Philippe
A1  - Heckel, Gerald
A1  - Ulrich, Rainer Günter
T1  - High genetic structuring of Tula hantavirus
JF  - Archives of virology
N2  - Tula virus (TULV) is a vole-associated hantavirus with low or no pathogenicity to humans. In the present study, 686 common voles (Microtus arvalis), 249 field voles (Microtus agrestis) and 30 water voles (Arvicola spec.) were collected at 79 sites in Germany, Luxembourg and France and screened by RT-PCR and TULV-IgG ELISA. TULV-specific RNA and/or antibodies were detected at 43 of the sites, demonstrating a geographically widespread distribution of the virus in the studied area. The TULV prevalence in common voles (16.7 %) was higher than that in field voles (9.2 %) and water voles (10.0 %). Time series data at ten trapping sites showed evidence of a lasting presence of TULV RNA within common vole populations for up to 34 months, although usually at low prevalence. Phylogenetic analysis demonstrated a strong genetic structuring of TULV sequences according to geography and independent of the rodent species, confirming the common vole as the preferential host, with spillover infections to co-occurring field and water voles. TULV phylogenetic clades showed a general association with evolutionary lineages in the common vole as assessed by mitochondrial DNA sequences on a large geographical scale, but with local-scale discrepancies in the contact areas.
Y1  - 2016
U6  - https://doi.org/10.1007/s00705-016-2762-6
SN  - 0304-8608
SN  - 1432-8798
VL  - 161
SP  - 1135
EP  - 1149
PB  - Springer
CY  - Wien
ER  -