TY  - JOUR
A1  - Gronau, Norbert
A1  - Korf, Roman
A1  - Müller, C.
T1  - KMDL - Capturing, analysing and improving knowledge-intensive business processes
N2  - Existing approaches in the area of knowledge-intensive processes focus on integrated knowledge and process management systems, the support of processes with KM systems, or the analysis of knowledge-intensive activities. For capturing knowledge-intensive business processes well known and established methods do not meet the requirements of a comprehensive and integrated approach of process-oriented knowledge management. These approaches are not able to visualise the decisions, actions and measures which are causing the sequence of the processes in an adequate manner. Parallel to conventional processes knowledge-intensive processes exist. These processes are based on conversions of knowledge within these processes. To fill these gaps in modelling knowledge-intensive business processes the Knowledge Modelling and Description Language (KMDL) got developed. The KMDL is able to represent the development, use, offer and demand of knowledge along business processes. Further it is possible to show the existing knowledge conversions which take place additionally to the normal business processes. The KMDL can be used to formalise knowledgeintensive processes with a focus on certain knowledge-specific characteristics and to identify process improvements in these processes. The KMDL modelling tool K-Modeler is introduced for a computer-aided modelling and analysing. The technical framework and the most important functionalities to support the analysis of the captured processes are introduced in the following contribution.
Y1  - 2005
UR  - http://wi.uni-potsdam.de
ER  - 
TY  - JOUR
A1  - Gronau, Norbert
A1  - Lämmer, Anne
A1  - Müller, C.
T1  - Selbstorganisierte Dienstleistungsnetzwerke im Maschinen- und Anlagenbau
Y1  - 2008
ER  - 
TY  - BOOK
A1  - Hailbronner, Kay
A1  - Magiera, S.
A1  - Klein, Eckart
A1  - Müller-Graf, C.
T1  - Handkommentar zum Vertrag über die Europäische Union : EUV, EGV
Y1  - 1996
SN  - 3-452-22171-7
PB  - Heymanns
CY  - Köln
ER  - 
TY  - JOUR
A1  - Harmeling, Stefan
A1  - Dornhege, Guido
A1  - Tax, David
A1  - Meinecke, Frank C.
A1  - Müller, Klaus-Robert
T1  - From outliers to prototypes : Ordering data
N2  - We propose simple and fast methods based on nearest neighbors that order objects from high-dimensional data sets from typical points to untypical points. On the one hand, we show that these easy-to-compute orderings allow us to detect outliers (i.e. very untypical points) with a performance comparable to or better than other often much more sophisticated methods. On the other hand, we show how to use these orderings to detect prototypes (very typical points) which facilitate exploratory data analysis algorithms such as noisy nonlinear dimensionality reduction and clustering. Comprehensive experiments demonstrate the validity of our approach.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/09252312
U6  - https://doi.org/10.1016/j.neucom.2005.05.015
SN  - 0925-2312
ER  - 
TY  - JOUR
A1  - Klein, Julia
A1  - Darvin, Maxim E.
A1  - Meinke, Martina C.
A1  - Schweigert, Florian J.
A1  - Müller, Kerstin E.
A1  - Lademann, Jürgen
T1  - Analyses of the correlation between dermal and blood carotenoids in female cattle by optical methods
JF  - Journal of biomedical optics
N2  - Herd health programs for the maintenance of welfare and productivity in cattle need efficient tools for monitoring the health of individual animals. Recent reports demonstrate that the oxidative status is related to various stress conditions in dairy cows. Biomarkers, among other carotenoids, could serve as indicators of stress originating from the environment (e.g., heat stress or sun radiation) or from the animal itself (e.g., disease). To date, only invasive in vitro tests are available to assess the oxidative status in cattle. The present study compares the results of optical noninvasive in vivo measurements of dermal carotenoids in cattle udder skin using an LED-based miniaturized spectroscopic system (MSS) with those obtained by photometric analysis of beta carotene in whole blood samples using a portable device. Correlations between the concentrations of dermal and blood carotenoids were calculated under consideration of the nutritional status of the animals. Significant correlation (R = 0.86) was found for cattle with a moderate to obese body condition. Thus, the blood and skin concentrations of the marker substance beta carotene are comparable under stable stress conditions of the cattle. This demonstrates that the MSS is suitable for noninvasive assessment of dermal carotenoid concentrations in cattle.
KW  - Raman spectroscopy
KW  - reflection spectroscopy
KW  - skin
KW  - antioxidants
KW  - free radicals
Y1  - 2013
U6  - https://doi.org/10.1117/1.JBO.18.6.061219
SN  - 1083-3668
VL  - 18
IS  - 6
PB  - SPIE
CY  - Bellingham
ER  - 
TY  - JOUR
A1  - Klaus, Valentin H.
A1  - Kleinebecker, Till
A1  - Prati, Daniel
A1  - Gossner, Martin M.
A1  - Alt, Fabian
A1  - Boch, Steffen
A1  - Gockel, Sonja
A1  - Hemp, Andreas
A1  - Lange, Markus
A1  - Müller, Jörg
A1  - Oelmann, Yvonne
A1  - Pasalic, Esther
A1  - Renner, Swen C.
A1  - Socher, Stephanie A.
A1  - Türke, Manfred
A1  - Weisser, Wolfgang W.
A1  - Fischer, Markus
A1  - Hölzel, Norbert
T1  - Does organic grassland farming benefit plant and arthropod diversity at the expense of yield and soil fertility?
JF  - Agriculture, ecosystems & environment : an international journal for scientific research on the relationship of agriculture and food production to the biosphere
N2  - Organic management is one of the most popular strategies to reduce negative environmental impacts of intensive agriculture. However, little is known about benefits for biodiversity and potential worsening of yield under organic grasslands management across different grassland types, i.e. meadow, pasture and mown pasture. Therefore, we studied the diversity of vascular plants and foliage-living arthropods (Coleoptera, Araneae, Heteroptera, Auchenorrhyncha), yield, fodder quality, soil phosphorus concentrations and land-use intensity of organic and conventional grasslands across three study regions in Germany. Furthermore, all variables were related to the time since conversion to organic management in order to assess temporal developments reaching up to 18 years. Arthropod diversity was significantly higher under organic than conventional management, although this was not the case for Araneae, Heteroptera and Auchenorrhyncha when analyzed separately. On the contrary, arthropod abundance, vascular plant diversity and also yield and fodder quality did not considerably differ between organic and conventional grasslands. Analyses did not reveal differences in the effect of organic management among grassland types. None of the recorded abiotic and biotic parameters showed a significant trend with time since transition to organic management, except soil organic phosphorus concentrations which decreased with time. This implies that permanent grasslands respond slower and probably weaker to organic management than crop fields do. However, as land-use intensity and inorganic soil phosphorus concentrations were significantly lower in organic grasslands, overcoming seed and dispersal limitation by re-introducing plant species might be needed to exploit the full ecological potential of organic grassland management. We conclude that although organic management did not automatically increase the diversity of all studied taxa, it is a reasonable and useful way to support agro-biodiversity.
KW  - Agri-environmental schemes
KW  - Fertilization
KW  - Fodder quality
KW  - Land-use intensity
KW  - Nitrogen
KW  - Biomass nutrient concentrations
KW  - Organic farming
KW  - Phosphorus
KW  - Species richness
KW  - Nutrient availability
Y1  - 2013
U6  - https://doi.org/10.1016/j.agee.2013.05.019
SN  - 0167-8809
VL  - 177
IS  - 3
SP  - 1
EP  - 9
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Blüthgen, Nico
A1  - Dormann, Carsten F.
A1  - Prati, Daniel
A1  - Klaus, Valentin H.
A1  - Kleinebecker, Till
A1  - Hoelzel, Norbert
A1  - Alt, Fabian
A1  - Boch, Steffen
A1  - Gockel, Sonja
A1  - Hemp, Andreas
A1  - Müller, Jörg
A1  - Nieschulze, Jens
A1  - Renner, Swen C.
A1  - Schöning, Ingo
A1  - Schumacher, Uta
A1  - Socher, Stephanie A.
A1  - Wells, Konstans
A1  - Birkhofer, Klaus
A1  - Buscot, Francois
A1  - Oelmann, Yvonne
A1  - Rothenwöhrer, Christoph
A1  - Scherber, Christoph
A1  - Tscharntke, Teja
A1  - Weiner, Christiane N.
A1  - Fischer, Markus
A1  - Kalko, Elisabeth K. V.
A1  - Linsenmair, Karl Eduard
A1  - Schulze, Ernst-Detlef
A1  - Weisser, Wolfgang W.
T1  - A quantitative index of land-use intensity in grasslands integrating mowing, grazing and fertilization
JF  - Basic and applied ecology : Journal of the Gesellschaft für Ökologie
N2  - Land use is increasingly recognized as a major driver of biodiversity and ecosystem functioning in many current research projects. In grasslands, land use is often classified by categorical descriptors such as pastures versus meadows or fertilized versus unfertilized sites. However, to account for the quantitative variation of multiple land-use types in heterogeneous landscapes, a quantitative, continuous index of land-use intensity (LUI) is desirable. Here we define such a compound, additive LUI index for managed grasslands including meadows and pastures. The LUI index summarizes the standardized intensity of three components of land use, namely fertilization, mowing, and livestock grazing at each site. We examined the performance of the LUI index to predict selected response variables on up to 150 grassland sites in the Biodiversity Exploratories in three regions in Germany(Alb, Hainich, Schorlheide). We tested the average Ellenberg nitrogen indicator values of the plant community, nitrogen and phosphorus concentration in the aboveground plant biomass, plant-available phosphorus concentration in the top soil, and soil C/N ratio, and the first principle component of these five response variables.
 The LUI index significantly predicted the principal component of all five response variables, as well as some of the individual responses. Moreover, vascular plant diversity decreased significantly with LUI in two regions (Alb and Hainich).
 Inter-annual changes in management practice were pronounced from 2006 to 2008, particularly due to variation in grazing intensity. This rendered the selection of the appropriate reference year(s) an important decision for analyses of land-use effects, whereas details in the standardization of the index were of minor importance. We also tested several alternative calculations of a LUI index, but all are strongly linearly correlated to the proposed index.
 The proposed LUI index reduces the complexity of agricultural practices to a single dimension and may serve as a baseline to test how different groups of organisms and processes respond to land use. In combination with more detailed analyses, this index may help to unravel whether and how land-use intensities, associated disturbance levels or other local or regional influences drive ecological processes.
KW  - Agro-ecosystems
KW  - Biodiversity exploratories
KW  - Grassland management
KW  - Land-use impacts
KW  - Livestock density
KW  - Meadows
KW  - Nitrogen
KW  - Pastures
Y1  - 2012
U6  - https://doi.org/10.1016/j.baae.2012.04.001
SN  - 1439-1791
VL  - 13
IS  - 3
SP  - 207
EP  - 220
PB  - Elsevier
CY  - Jena
ER  - 
TY  - JOUR
A1  - Thulke, Hans-Hermann
A1  - Tischendorf, L.
A1  - Staubach, C.
A1  - Selhorst, T.
A1  - Jeltsch, Florian
A1  - Müller, T.
A1  - Schlüter, H.
A1  - Wissel, Christian
T1  - The spatio-temporal dynamics of a post-vaccination recovery of rabies in foxes and emergency vaccination planning
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Parra, L.
A1  - Spence, C.
A1  - Sajda, P.
A1  - Ziehe, Andreas
A1  - Müller, Klaus-Robert
T1  - Unmixing hyperspectral data
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Gomez-Merino, Fernando Carlos
A1  - Arana-Ceballos, Fernando Alberto
A1  - Trejo-Tellez, L. I.
A1  - Skirycz, Aleksandra
A1  - Brearley, C. A.
A1  - Dormann, P.
A1  - Müller-Röber, Bernd
T1  - Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration : the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development
N2  - Diacylglycerol kinase (DGK) regulates the level of the second messenger diacylglycerol and produces phosphatidic acid (PA), another signaling molecule. The Arabidopsis thaliana genome encodes seven putative diacylglycerol kinase isozymes (named AtDGK1 to -7), structurally falling into three major clusters. So far, enzymatic activity has not been reported for any plant Cluster II DGK. Here, we demonstrate that a representative of this cluster, AtDGK7, is biochemically active when expressed as a recombinant protein in Escherichia coli. AtDGK7, encoded by gene locus At4g30340, contains 374 amino acids with an apparent molecular mass of 41.2 kDa. AtDGK7 harbors an N-terminal catalytic domain, but in contrast to various characterized DGKs (including AtDGK2), it lacks a cysteine-rich domain at its N terminus, and, importantly, its C-terminal DGK accessory domain is incomplete. Recombinant AtDGK7 expressed in E. coli exhibits Michaelis-Menten type kinetics with 1,2-dioleoyl-sn-glycerol as substrate. AtDGK7 activity was affected by pH, detergents, and the DGK inhibitor R59022. We demonstrate that both AtDGK2 and AtDGK7 phosphorylate diacylglycerol molecular species that are typically found in plants, indicating that both enzymes convert physiologically relevant substrates. AtDGK7 is expressed throughout the Arabidopsis plant, but expression is strongest in flowers and young seedlings. Expression of AtDGK2 is transiently induced by wounding. R59022 at similar to 80 mu M inhibits root elongation and lateral root formation and reduces plant growth, indicating that DGKs play an important role in plant development
Y1  - 2005
SN  - 0021-9258
ER  - 
TY  - JOUR
A1  - Lin, W. H.
A1  - Wang, Y.
A1  - Müller-Röber, Bernd
A1  - Brearley, C. A.
A1  - Xu, Z. H.
A1  - Xue, H. W.
T1  - At5PTase13 modulates cotyledon vein development through regulating auxin homeostasis
N2  - Phosphatidylinositol signaling pathway and the relevant metabolites are known to be critical to the modulation of different aspects of plant growth, development, and stress responses. Inositol polyphosphate 5-phosphatase is a key enzyme involved in phosphatidylinositol metabolism and is encoded by an At5PTase gene family in Arabidopsis thaliana. A previous study shows that At5PTase11 mediates cotyledon vascular development probably through the regulation of intracellular calcium levels. In this study, we provide evidence that At5PTase13 modulates the development of cotyledon veins through its regulation of auxin homeostasis. A T-DNA insertional knockout mutant, At5pt13-1, showed a defect in development of the cotyledon vein, which was rescued completely by exogenous auxin and in part by brassinolide, a steroid hormone. Furthermore, the mutant had reduced auxin content and altered auxin accumulation in seedlings revealed by the DR5:beta-glucuronidase fusion construct in seedlings. In addition, microarray analysis shows that the transcription of key genes responsible for auxin biosynthesis and transport was altered in At5pt13-1. The At5pt13-1 mutant was also less sensitive to auxin inhibition of root elongation. These results suggest that At5PTase13 regulates the homeostasis of auxin, a key hormone controlling vascular development in plants
Y1  - 2005
SN  - 0032-0889
ER  - 
TY  - JOUR
A1  - Gomez-Merino, Fernando Carlos
A1  - Brearley, C. A.
A1  - Ornatowska, Magdalena
A1  - Abdel-Haliem, Mahmoud E. F.
A1  - Zanor, Maria Ines
A1  - Müller-Röber, Bernd
T1  - AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn- glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression
N2  - Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Both DAG and PA are implicated in signal transduction pathways. DGKs have been widely studied in animals, but their analysis in plants is fragmentary. Here, we report the cloning and biochemical characterization of AtDGK2, encoding DGK from Arabidopsis thaliana. AtDGK2 has a predicted molecular mass of 79.4 kDa and, like AtDGK1 previously reported, harbors two copies of a phorbol ester/DAG-binding domain in its N-terminal region. AtDGK2 belongs to a family of seven DGK genes in A. thaliana. AtDGK3 to AtDGK7 encode similar to55-kDa DGKs that lack a typical phorbol ester/DAG-binding domain. Phylogenetically, plant DGKs fall into three clusters. Members of all three clusters are widely expressed in vascular plants. Recombinant AtDGK2 was expressed in Escherichia coli and biochemically characterized. The enzyme phosphorylated 1,2-dioleoyl-sn-glycerol to yield PA, exhibiting Michaelis-Menten type kinetics. Estimated K-m and V-max values were 125 muM for DAG and 0.25 pmol of PA min(-1) mug(-1), respectively. The enzyme was maximally active at pH 7.2. Its activity was Mg2+-dependent and affected by the presence of detergents, salts, and the DGK inhibitor R59022, but not by Ca2+. AtDGK2 exhibited substrate preference for unsaturated DAG analogues (i.e. 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2- dioleoyl-sn-glycerol). The AtDGK2 gene is expressed in various tissues of the Arabidopsis plant, including leaves, roots, and flowers, as shown by Northern blot analysis and promoter-reporter gene fusions. We found that AtDGK2 is induced by exposure to low temperature (4degreesC), pointing to a role in cold signal transduction
Y1  - 2004
SN  - 0021-9258
ER  - 
TY  - JOUR
A1  - Xu, J.
A1  - Brearley, C. A.
A1  - Lin, W. H.
A1  - Wang, Y.
A1  - Ye, R.
A1  - Müller-Röber, Bernd
A1  - Xu, Z. H.
A1  - Xue, H. W.
T1  - A role of Arabidopsis inositol polyphosphate kinase, AtIPK2 alpha, in pollen germination and root growth
N2  - Inositol polyphosphates, such as inositol trisphosphate, are pivotal intracellular signaling molecules in eukaryotic cells. In higher plants the mechanism for the regulation of the type and the level of these signaling molecules is poorly understood. In this study we investigate the physiological function of an Arabidopsis (Arabidopsis thaliana) gene encoding inositol polyphosphate kinase (AtIPK2alpha), which phosphorylates inositol 1,4,5-trisphosphate successively at the D-6 and D-3 positions, and inositol 1,3,4,5-tetrakisphosphate at D-6, resulting in the generation of inositol 1,3,4,5,6-pentakisphosphate. Semiquantitative reverse transcription-PCR and promoter-beta-glucuronidase reporter gene analyses showed that AtIPK2alpha is expressed in various tissues, including roots and root hairs, stem, leaf, pollen grains, pollen tubes, the flower stigma, and siliques. Transgenic Arabidopsis plants expressing the AtIPK2alpha antisense gene under its own promoter were generated. Analysis of several independent transformants exhibiting strong reduction in AtIPK2alpha transcript levels showed that both pollen germination and pollen tube growth were enhanced in the antisense lines compared to wild-type plants, especially in the presence of nonoptimal low Ca2+ concentrations in the culture medium. Furthermore, root growth and root hair development were also stimulated in the antisense lines, in the presence of elevated external Ca2+ concentration or upon the addition of EGTA. In addition, seed germination and early seedling growth was stimulated in the antisense lines. These observations suggest a general and important role of AtIPK2alpha, and hence inositol polyphosphate metabolism, in the regulation of plant growth most likely through the regulation of calcium signaling, consistent with the well-known function of inositol trisphosphate in the mobilization of intracellular calcium stores
Y1  - 2005
SN  - 0032-0889
ER  - 
TY  - JOUR
A1  - Diluiso, Francesca
A1  - Walk, Paula
A1  - Manych, Niccolo
A1  - Cerutti, Nicola
A1  - Chipiga, Vladislav
A1  - Workman, Annabelle
A1  - Ayas, Ceren
A1  - Cui, Ryna Yiyun
A1  - Cui, Diyang
A1  - Song, Kaihui
A1  - Banisch, Lucy A.
A1  - Moretti, Nikolaj
A1  - Callaghan, Max W.
A1  - Clarke, Leon
A1  - Creutzig, Felix
A1  - Hilaire, Jerome
A1  - Jotzo, Frank
A1  - Kalkuhl, Matthias
A1  - Lamb, William F.
A1  - Löschel, Andreas
A1  - Müller-Hansen, Finn
A1  - Nemet, Gregory F.
A1  - Oei, Pao-Yu
A1  - Sovacool, Benjamin K.
A1  - Steckel, Jan Christoph
A1  - Thomas, Sebastian
A1  - Wiseman, John
A1  - Minx, Jan C.
T1  - Coal transitions - part 1
BT  - a systematic map and review of case study learnings from regional, national, and local coal phase-out experiences
JF  - Environmental research letters
N2  - A rapid coal phase-out is needed to meet the goals of the Paris Agreement, but is hindered by serious challenges ranging from vested interests to the risks of social disruption. To understand how to organize a global coal phase-out, it is crucial to go beyond cost-effective climate mitigation scenarios and learn from the experience of previous coal transitions. Despite the relevance of the topic, evidence remains fragmented throughout different research fields, and not easily accessible. To address this gap, this paper provides a systematic map and comprehensive review of the literature on historical coal transitions. We use computer-assisted systematic mapping and review methods to chart and evaluate the available evidence on historical declines in coal production and consumption. We extracted a dataset of 278 case studies from 194 publications, covering coal transitions in 44 countries and ranging from the end of the 19th century until 2021. We find a relatively recent and rapidly expanding body of literature reflecting the growing importance of an early coal phase-out in scientific and political debates. Previous evidence has primarily focused on the United Kingdom, the United States, and Germany, while other countries that experienced large coal declines, like those in Eastern Europe, are strongly underrepresented. An increasing number of studies, mostly published in the last 5 years, has been focusing on China. Most of the countries successfully reducing coal dependency have undergone both demand-side and supply-side transitions. This supports the use of policy approaches targeting both demand and supply to achieve a complete coal phase-out. From a political economy perspective, our dataset highlights that most transitions are driven by rising production costs for coal, falling prices for alternative energies, or local environmental concerns, especially regarding air pollution. The main challenges for coal-dependent regions are structural change transformations, in particular for industry and labor. Rising unemployment is the most largely documented outcome in the sample. Policymakers at multiple levels are instrumental in facilitating coal transitions. They rely mainly on regulatory instruments to foster the transitions and compensation schemes or investment plans to deal with their transformative processes. Even though many models suggest that coal phase-outs are among the low-hanging fruits on the way to climate neutrality and meeting the international climate goals, our case studies analysis highlights the intricate political economy at work that needs to be addressed through well-designed and just policies.
KW  - climate change mitigation
KW  - coal transitions
KW  - evidence synthesis
KW  - political economy
KW  - systematic map
Y1  - 2021
U6  - https://doi.org/10.1088/1748-9326/ac1b58
SN  - 1748-9326
VL  - 16
IS  - 11
PB  - Institute of Physics Publishing (IOP)
CY  - Bristol
ER  - 
TY  - JOUR
A1  - Becker, Dirk
A1  - Geiger, D.
A1  - Dunkel, M.
A1  - Roller, A.
A1  - Bertl, Adam
A1  - Latz, A.
A1  - Carpaneto, Armando
A1  - Dietrich, Peter
A1  - Roelfsema, M. R. G.
A1  - Voelker, C.
A1  - Schmidt, D.
A1  - Müller-Röber, Bernd
A1  - Czempinski, Katrin
A1  - Hedrich, R.
T1  - AtTPK4, an Arabidopsis tandem-pore K+ channel, poised to control the pollen membrane voltage in a pH- and Ca2+- dependent manner
N2  - The Arabidopsis tandem-pore K+ (TPK) channels displaying four transmembrane domains and two pore regions share structural homologies with their animal counterparts of the KCNK family. In contrast to the Shaker-like Arabidopsis channels (six transmembrane domains/one pore region), the functional properties and the biological role of plant TPK channels have not been elucidated yet. Here, we show that AtTPK4 (KCO4) localizes to the plasma membrane and is predominantly expressed in pollen. AtTPK4 (KCO4) resembles the electrical properties of a voltage-independent K+ channel after expression in Xenopus oocytes and yeast. Hyperpolarizing as well as depolarizing membrane voltages elicited instantaneous K+ currents, which were blocked by extracellular calcium and cytoplasmic protons. Functional complementation assays using a K+ transport-deficient yeast confirmed the biophysical and pharmacological properties of the AtTPK4 channel. The features of AtTPK4 point toward a role in potassium homeostasis and membrane voltage control of the growing pollen tube. Thus, AtTPK4 represents a member of plant tandem-pore-K+ channels, resembling the characteristics of its animal counterparts as well as plant-specific features with respect to modulation of channel activity by acidosis and calcium
Y1  - 2004
SN  - 0027-8424
ER  - 
TY  - JOUR
A1  - Mehrabi, Pedram
A1  - Schulz, Eike C.
A1  - Dsouza, Raison
A1  - Müller-Werkmeister, Henrike
A1  - Tellkamp, Friedjof
A1  - Miller, R. J. Dwayne
A1  - Pai, Emil F.
T1  - Time-resolved crystallography reveals allosteric communication aligned with molecular breathing
JF  - Science
N2  - A comprehensive understanding of protein function demands correlating structure and dynamic changes. Using time-resolved serial synchrotron crystallography, we visualized half-of-the-sites reactivity and correlated molecular-breathing motions in the enzyme fluoroacetate dehalogenase. Eighteen time points from 30 milliseconds to 30 seconds cover four turnover cycles of the irreversible reaction. They reveal sequential substrate binding, covalent-intermediate formation, setup of a hydrolytic water molecule, and product release. Small structural changes of the protein mold and variations in the number and placement of water molecules accompany the various chemical steps of catalysis. Triggered by enzyme-ligand interactions, these repetitive changes in the protein framework’s dynamics and entropy constitute crucial components of the catalytic machinery.
Y1  - 2019
U6  - https://doi.org/10.1126/science.aaw9904
SN  - 0036-8075
SN  - 1095-9203
VL  - 365
IS  - 6458
SP  - 1167
EP  - 1170
PB  - American Assoc. for the Advancement of Science
CY  - Washington
ER  - 
TY  - JOUR
A1  - Schulz, Eike C.
A1  - Mehrabi, Pedram
A1  - Müller-Werkmeister, Henrike
A1  - Tellkamp, Friedjof
A1  - Jha, Ajay
A1  - Stuart, William
A1  - Persch, Elke
A1  - De Gasparo, Raoul
A1  - Diederich, François
A1  - Pai, Emil F.
A1  - Miller, R. J. Dwayne
T1  - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
JF  - Nature methods : techniques for life scientists and chemists
N2  - We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
KW  - Biophysical chemistry
KW  - Enzymes
KW  - Molecular biophysics
KW  - X-ray crystallography
Y1  - 2018
U6  - https://doi.org/10.1038/s41592-018-0180-2
SN  - 1548-7091
SN  - 1548-7105
VL  - 15
IS  - 11
SP  - 901
EP  - 904
PB  - Nature Publishing Group (London)
CY  - London
ER  - 
TY  - JOUR
A1  - Wienhold, Sandra-Maria
A1  - Macri, Mario
A1  - Nouailles, Geraldine
A1  - Dietert, Kristina
A1  - Gurtner, Corinne
A1  - Gruber, Achim D.
A1  - Heimesaat, Markus M.
A1  - Lienau, Jasmin
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Opitz, Bastian
A1  - Suttorp, Norbert
A1  - Witzenrath, Martin
A1  - Müller-Redetzky, Holger C.
T1  - Ventilator-induced lung injury is aggravated by antibiotic mediated microbiota depletion in mice
JF  - Critical Care
N2  - BackgroundAntibiotic exposure alters the microbiota, which can impact the inflammatory immune responses. Critically ill patients frequently receive antibiotic treatment and are often subjected to mechanical ventilation, which may induce local and systemic inflammatory responses and development of ventilator-induced lung injury (VILI). The aim of this study was to investigate whether disruption of the microbiota by antibiotic therapy prior to mechanical ventilation affects pulmonary inflammatory responses and thereby the development of VILI.MethodsMice underwent 6-8weeks of enteral antibiotic combination treatment until absence of cultivable bacteria in fecal samples was confirmed. Control mice were housed equally throughout this period. VILI was induced 3 days after completing the antibiotic treatment protocol, by high tidal volume (HTV) ventilation (34ml/kg; positive end-expiratory pressure=2 cmH(2)O) for 4h. Differences in lung function, oxygenation index, pulmonary vascular leakage, macroscopic assessment of lung injury, and leukocyte and lymphocyte differentiation were assessed. Control groups of mice ventilated with low tidal volume and non-ventilated mice were analyzed accordingly.ResultsAntibiotic-induced microbiota depletion prior to HTV ventilation led to aggravation of VILI, as shown by increased pulmonary permeability, increased oxygenation index, decreased pulmonary compliance, enhanced macroscopic lung injury, and increased cytokine/chemokine levels in lung homogenates.ConclusionsDepletion of the microbiota by broad-spectrum antibiotics prior to HTV ventilation renders mice more susceptible to developing VILI, which could be clinically relevant for critically ill patients frequently receiving broad-spectrum antibiotics.
KW  - Broad-spectrum antibiotic therapy
KW  - Ventilator-induced lung injury
KW  - Microbiota
Y1  - 2018
U6  - https://doi.org/10.1186/s13054-018-2213-8
SN  - 1466-609X
SN  - 1364-8535
VL  - 22
IS  - 282
PB  - BMC
CY  - London
ER  - 
TY  - JOUR
A1  - Gulbins, Anne
A1  - Schumacher, Fabian
A1  - Becker, Katrin Anne
A1  - Wilker, Barbara
A1  - Soddemann, Matthias
A1  - Boldrin, Francesco
A1  - Müller, Christian P.
A1  - Edwards, Michael J.
A1  - Goodman, Michael
A1  - Caldwell, Charles C.
A1  - Kleuser, Burkhard
A1  - Kornhuber, Johannes
A1  - Szabo, Ildiko
A1  - Gulbins, Erich
T1  - Antidepressants act by inducing autophagy controlled by sphingomyelin-ceramide
JF  - Molecular psychiatry
N2  - Major depressive disorder (MDD) is a common and severe disease characterized by mood changes, somatic alterations, and often suicide. MDD is treated with antidepressants, but the molecular mechanism of their action is unknown. We found that widely used antidepressants such as amitriptyline and fluoxetine induce autophagy in hippocampal neurons via the slow accumulation of sphingomyelin in lysosomes and Golgi membranes and of ceramide in the endoplasmic reticulum (ER). ER ceramide stimulates phosphatase 2A and thereby the autophagy proteins Ulk, Beclin, Vps34/Phosphatidylinositol 3-kinase, p62, and Lc3B. Although treatment with amitriptyline or fluoxetine requires at least 12 days to achieve sphingomyelin accumulation and the subsequent biochemical and cellular changes, direct inhibition of sphingomyelin synthases with tricyclodecan-9-yl-xanthogenate (D609) results in rapid (within 3 days) accumulation of ceramide in the ER, activation of autophagy, and reversal of biochemical and behavioral signs of stress-induced MDD. Inhibition of Beclin blocks the antidepressive effects of amitriptyline and D609 and induces cellular and behavioral changes typical of MDD. These findings identify sphingolipid-controlled autophagy as an important target for antidepressive treatment methods and provide a rationale for the development of novel antidepressants that act within a few days.
Y1  - 2018
U6  - https://doi.org/10.1038/s41380-018-0090-9
SN  - 1359-4184
SN  - 1476-5578
VL  - 23
IS  - 12
SP  - 2324
EP  - 2346
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - GEN
A1  - Troll, K.
A1  - Kulkarni, Amit
A1  - Wang, W.
A1  - Darko, C.
A1  - Koumba, A. M. Bivigou
A1  - Laschewsky, André
A1  - Müller-Buschbaum, Peter
A1  - Papadakis, Christine M.
T1  - The collapse transition of poly(styrene-b-(N-isopropyl acrylamide)) diblock copolymers in aqueous solution and in thin films
T2  - Colloid and polymer science : official journal of the Kolloid-Gesellschaft
Y1  - 2011
U6  - https://doi.org/10.1007/s00396-010-2344-1
SN  - 0303-402X
VL  - 289
IS  - 2
SP  - 227
EP  - 227
PB  - Springer
CY  - New York
ER  -