TY  - JOUR
A1  - Christakoudi, Sofa
A1  - Tsilidis, Konstantinos K.
A1  - Muller, David C.
A1  - Freisling, Heinz
A1  - Weiderpass, Elisabete
A1  - Overvad, Kim
A1  - Söderberg, Stefan
A1  - Häggström, Christel
A1  - Pischon, Tobias
A1  - Dahm, Christina C.
A1  - Zhang, Jie
A1  - Tjønneland, Anne
A1  - Schulze, Matthias Bernd
T1  - A Body Shape Index (ABSI) achieves better mortality risk stratification than alternative indices of abdominal obesity: results from a large European cohort
JF  - Scientific Reports
N2  - Abdominal and general adiposity are independently associated with mortality, but there is no consensus on how best to assess abdominal adiposity. We compared the ability of alternative waist indices to complement body mass index (BMI) when assessing all-cause mortality. We used data from 352,985 participants in the European Prospective Investigation into Cancer and Nutrition (EPIC) and Cox proportional hazards models adjusted for other risk factors. During a mean follow-up of 16.1 years, 38,178 participants died. Combining in one model BMI and a strongly correlated waist index altered the association patterns with mortality, to a predominantly negative association for BMI and a stronger positive association for the waist index, while combining BMI with the uncorrelated A Body Shape Index (ABSI) preserved the association patterns. Sex-specific cohort-wide quartiles of waist indices correlated with BMI could not separate high-risk from low-risk individuals within underweight (BMI<18.5 kg/m(2)) or obese (BMI30 kg/m(2)) categories, while the highest quartile of ABSI separated 18-39% of the individuals within each BMI category, which had 22-55% higher risk of death. In conclusion, only a waist index independent of BMI by design, such as ABSI, complements BMI and enables efficient risk stratification, which could facilitate personalisation of screening, treatment and monitoring.
KW  - all-cause mortality
KW  - anthropometric measures
KW  - mass index
KW  - overweight
KW  - cancer
KW  - prediction
KW  - adiposity
KW  - size
Y1  - 2020
VL  - 10
IS  - 1
PB  - Springer Nature
CY  - Berlin
ER  - 
TY  - GEN
A1  - Christakoudi, Sofa
A1  - Tsilidis, Konstantinos K.
A1  - Muller, David C.
A1  - Freisling, Heinz
A1  - Weiderpass, Elisabete
A1  - Overvad, Kim
A1  - Söderberg, Stefan
A1  - Häggström, Christel
A1  - Pischon, Tobias
A1  - Dahm, Christina C.
A1  - Zhang, Jie
A1  - Tjønneland, Anne
A1  - Schulze, Matthias Bernd
T1  - A Body Shape Index (ABSI) achieves better mortality risk stratification than alternative indices of abdominal obesity: results from a large European cohort
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Abdominal and general adiposity are independently associated with mortality, but there is no consensus on how best to assess abdominal adiposity. We compared the ability of alternative waist indices to complement body mass index (BMI) when assessing all-cause mortality. We used data from 352,985 participants in the European Prospective Investigation into Cancer and Nutrition (EPIC) and Cox proportional hazards models adjusted for other risk factors. During a mean follow-up of 16.1 years, 38,178 participants died. Combining in one model BMI and a strongly correlated waist index altered the association patterns with mortality, to a predominantly negative association for BMI and a stronger positive association for the waist index, while combining BMI with the uncorrelated A Body Shape Index (ABSI) preserved the association patterns. Sex-specific cohort-wide quartiles of waist indices correlated with BMI could not separate high-risk from low-risk individuals within underweight (BMI<18.5 kg/m(2)) or obese (BMI30 kg/m(2)) categories, while the highest quartile of ABSI separated 18-39% of the individuals within each BMI category, which had 22-55% higher risk of death. In conclusion, only a waist index independent of BMI by design, such as ABSI, complements BMI and enables efficient risk stratification, which could facilitate personalisation of screening, treatment and monitoring.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1200 
KW  - all-cause mortality
KW  - anthropometric measures
KW  - mass index
KW  - overweight
KW  - cancer
KW  - prediction
KW  - adiposity
KW  - size
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-525827
SN  - 1866-8372
IS  - 1
ER  - 
TY  - JOUR
A1  - Gross, M.
A1  - Müller, David C.
A1  - Nothofer, Heinz-Georg
A1  - Scherf, Ullrich
A1  - Neher, Dieter
A1  - Bräuchler, C.
A1  - Meerholz, Klaus
T1  - Improving the performance of doped p-conjugated polymers for use in organic light-emitting diodes
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Gossner, Martin M.
A1  - Lewinsohn, Thomas M.
A1  - Kahl, Tiemo
A1  - Grassein, Fabrice
A1  - Boch, Steffen
A1  - Prati, Daniel
A1  - Birkhofer, Klaus
A1  - Renner, Swen C.
A1  - Sikorski, Johannes
A1  - Wubet, Tesfaye
A1  - Arndt, Hartmut
A1  - Baumgartner, Vanessa
A1  - Blaser, Stefan
A1  - Blüthgen, Nico
A1  - Börschig, Carmen
A1  - Buscot, Francois
A1  - Diekötter, Tim
A1  - Jorge, Leonardo Re
A1  - Jung, Kirsten
A1  - Keyel, Alexander C.
A1  - Klein, Alexandra-Maria
A1  - Klemmer, Sandra
A1  - Krauss, Jochen
A1  - Lange, Markus
A1  - Müller, Jörg
A1  - Overmann, Jörg
A1  - Pasalic, Esther
A1  - Penone, Caterina
A1  - Perovic, David J.
A1  - Purschke, Oliver
A1  - Schall, Peter
A1  - Socher, Stephanie A.
A1  - Sonnemann, Ilja
A1  - Tschapka, Marco
A1  - Tscharntke, Teja
A1  - Türke, Manfred
A1  - Venter, Paul Christiaan
A1  - Weiner, Christiane N.
A1  - Werner, Michael
A1  - Wolters, Volkmar
A1  - Wurst, Susanne
A1  - Westphal, Catrin
A1  - Fischer, Markus
A1  - Weisser, Wolfgang W.
A1  - Allan, Eric
T1  - Land-use intensification causes multitrophic homogenization of grassland communities
JF  - Nature : the international weekly journal of science
N2  - Land-use intensification is a major driver of biodiversity loss(1,2). Alongside reductions in local species diversity, biotic homogenization at larger spatial scales is of great concern for conservation. Biotic homogenization means a decrease in beta-diversity (the compositional dissimilarity between sites). Most studies have investigated losses in local (alpha)-diversity(1,3) and neglected biodiversity loss at larger spatial scales. Studies addressing beta-diversity have focused on single or a few organism groups (for example, ref. 4), and it is thus unknown whether land-use intensification homogenizes communities at different trophic levels, above-and belowground. Here we show that even moderate increases in local land-use intensity (LUI) cause biotic homogenization across microbial, plant and animal groups, both above- and belowground, and that this is largely independent of changes in alpha-diversity. We analysed a unique grassland biodiversity dataset, with abundances of more than 4,000 species belonging to 12 trophic groups. LUI, and, in particular, high mowing intensity, had consistent effects on beta-diversity across groups, causing a homogenization of soil microbial, fungal pathogen, plant and arthropod communities. These effects were nonlinear and the strongest declines in beta-diversity occurred in the transition from extensively managed to intermediate intensity grassland. LUI tended to reduce local alpha-diversity in aboveground groups, whereas the alpha-diversity increased in belowground groups. Correlations between the alpha-diversity of different groups, particularly between plants and their consumers, became weaker at high LUI. This suggests a loss of specialist species and is further evidence for biotic homogenization. The consistently negative effects of LUI on landscape-scale biodiversity underscore the high value of extensively managed grasslands for conserving multitrophic biodiversity and ecosystem service provision. Indeed, biotic homogenization rather than local diversity loss could prove to be the most substantial consequence of land-use intensification.
Y1  - 2016
U6  - https://doi.org/10.1038/nature20575
SN  - 0028-0836
SN  - 1476-4687
VL  - 540
SP  - 266
EP  - +
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Allan, Eric
A1  - Bossdorf, Oliver
A1  - Dormann, Carsten F.
A1  - Prati, Daniel
A1  - Gossner, Martin M.
A1  - Tscharntke, Teja
A1  - Blüthgen, Nico
A1  - Bellach, Michaela
A1  - Birkhofer, Klaus
A1  - Boch, Steffen
A1  - Böhm, Stefan
A1  - Börschig, Carmen
A1  - Chatzinotas, Antonis
A1  - Christ, Sabina
A1  - Daniel, Rolf
A1  - Diekötter, Tim
A1  - Fischer, Christiane
A1  - Friedl, Thomas
A1  - Glaser, Karin
A1  - Hallmann, Christine
A1  - Hodac, Ladislav
A1  - Hölzel, Norbert
A1  - Jung, Kirsten
A1  - Klein, Alexandra-Maria
A1  - Klaus, Valentin H.
A1  - Kleinebecker, Till
A1  - Krauss, Jochen
A1  - Lange, Markus
A1  - Morris, E. Kathryn
A1  - Müller, Jörg
A1  - Nacke, Heiko
A1  - Pasalic, Esther
A1  - Rillig, Matthias C.
A1  - Rothenwoehrer, Christoph
A1  - Schally, Peter
A1  - Scherber, Christoph
A1  - Schulze, Waltraud X.
A1  - Socher, Stephanie A.
A1  - Steckel, Juliane
A1  - Steffan-Dewenter, Ingolf
A1  - Türke, Manfred
A1  - Weiner, Christiane N.
A1  - Werner, Michael
A1  - Westphal, Catrin
A1  - Wolters, Volkmar
A1  - Wubet, Tesfaye
A1  - Gockel, Sonja
A1  - Gorke, Martin
A1  - Hemp, Andreas
A1  - Renner, Swen C.
A1  - Schöning, Ingo
A1  - Pfeiffer, Simone
A1  - König-Ries, Birgitta
A1  - Buscot, Francois
A1  - Linsenmair, Karl Eduard
A1  - Schulze, Ernst-Detlef
A1  - Weisser, Wolfgang W.
A1  - Fischer, Markus
T1  - Interannual variation in land-use intensity enhances grassland multidiversity
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - Although temporal heterogeneity is a well-accepted driver of biodiversity, effects of interannual variation in land-use intensity (LUI) have not been addressed yet. Additionally, responses to land use can differ greatly among different organisms; therefore, overall effects of land-use on total local biodiversity are hardly known. To test for effects of LUI (quantified as the combined intensity of fertilization, grazing, and mowing) and interannual variation in LUI (SD in LUI across time), we introduce a unique measure of whole-ecosystem biodiversity, multidiversity. This synthesizes individual diversity measures across up to 49 taxonomic groups of plants, animals, fungi, and bacteria from 150 grasslands. Multidiversity declined with increasing LUI among grasslands, particularly for rarer species and aboveground organisms, whereas common species and belowground groups were less sensitive. However, a high level of interannual variation in LUI increased overall multidiversity at low LUI and was even more beneficial for rarer species because it slowed the rate at which the multidiversity of rare species declined with increasing LUI. In more intensively managed grasslands, the diversity of rarer species was, on average, 18% of the maximum diversity across all grasslands when LUI was static over time but increased to 31% of the maximum when LUI changed maximally over time. In addition to decreasing overall LUI, we suggest varying LUI across years as a complementary strategy to promote biodiversity conservation.
KW  - biodiversity loss
KW  - agricultural grasslands
KW  - Biodiversity Exploratories
Y1  - 2014
U6  - https://doi.org/10.1073/pnas.1312213111
SN  - 0027-8424
VL  - 111
IS  - 1
SP  - 308
EP  - 313
PB  - National Acad. of Sciences
CY  - Washington
ER  - 
TY  - JOUR
A1  - Müller, C.
A1  - Dibbern, P
T1  - Selbstorganisiertes Wissensmanagement in Unternehmen auf Basis der Wiki-Technologie : ein Anwendungsfall
Y1  - 2006
SN  - 978-3- 89864-384-9
ER  - 
TY  - JOUR
A1  - Müller, C.
A1  - Birn, Lukas
T1  - Wikis for collaborative software documentation
Y1  - 2006
ER  - 
TY  - JOUR
A1  - Meinecke, Frank C.
A1  - Ziehe, Andreas
A1  - Kurths, Jürgen
A1  - Müller, Klaus-Robert
T1  - Measuring phase synchronization of superimposed signals
N2  - Phase synchronization is an important phenomenon that occurs in a wide variety of complex oscillatory processes. Measuring phase synchronization can therefore help to gain fundamental insight into nature. In this Letter we point out that synchronization analysis techniques can detect spurious synchronization, if they are fed with a superposition of signals such as in electroencephalography or magnetoencephalography data. We show how techniques from blind source separation can help to nevertheless measure the true synchronization and avoid such pitfalls
Y1  - 2005
SN  - 0031-9007
ER  - 
TY  - JOUR
A1  - Nolte, Guido
A1  - Meinecke, Frank C.
A1  - Ziehe, Andreas
A1  - Müller, Klaus-Robert
T1  - Identifying interactions in mixed and noisy complex systems
N2  - We present a technique that identifies truly interacting subsystems of a complex system from multichannel data if the recordings are an unknown linear and instantaneous mixture of the true sources. The method is valid for arbitrary noise structure. For this, a blind source separation technique is proposed that diagonalizes antisymmetrized cross- correlation or cross-spectral matrices. The resulting decomposition finds truly interacting subsystems blindly and suppresses any spurious interaction stemming from the mixture. The usefulness of this interacting source analysis is demonstrated in simulations and for real electroencephalography data
Y1  - 2006
UR  - http://pre.aps.org/
U6  - https://doi.org/10.1103/Physreve.73.051913
ER  - 
TY  - JOUR
A1  - Bahrs, Julian
A1  - Müller, C.
T1  - Wissensnachfrage-orientierte Gestaltung von Softwareentwicklungsprozessen
N2  - Die zunehmend dynamische Umwelt erfordert steigende Adaptivität von Softwareentwicklungsprozessen. Dem kann durch eine Prozessgestaltung nach Methoden des geschäftsprozessorientierten Wissensmanagements und dem damit vollzogenen Paradigmenwechsel zur nachfrageorientierten Wissensverteilung begegnet werden. In diesem Beitrag wird die Analyse einer ausgewählten Instanz eines mit der KMDL-SE beschriebenen Softwareentwicklungsprozesses vorgestellt
Y1  - 2005
UR  - http://wi.uni-potsdam.de
ER  - 
TY  - JOUR
A1  - Meinecke, Frank C.
A1  - Harmeling, Stefan
A1  - Müller, Klaus-Robert
T1  - Inlier-based ICA with an application to superimposed images
N2  - This paper proposes a new independent component analysis (ICA) method which is able to unmix overcomplete mixtures of sparce or structured signals like speech, music or images. Furthermore, the method is designed to be robust against outliers, which is a favorable feature for ICA algorithms since most of them are extremely sensitive to outliers. Our approach is based on a simple outlier index. However, instead of robustifying an existing algorithm by some outlier rejection technique we show how this index can be used directly to solve the ICA problem for super-Gaussian sources. The resulting inlier-based ICA (IBICA) is outlier-robust by construction and can be used for standard ICA as well as for overcomplete ICA (i.e. more source signals than observed signals). (c) 2005 Wiley Periodicals, Inc
Y1  - 2005
SN  - 0899-9457
ER  - 
TY  - JOUR
A1  - Harmeling, Stefan
A1  - Meinecke, Frank C.
A1  - Müller, Klaus-Robert
T1  - Injecting noise for analysing the stability of ICA components
N2  - Usually, noise is considered to be destructive. We present a new method that constructively injects noise to assess the reliability and the grouping structure of empirical ICA component estimates. Our method can be viewed as a Monte-Carlo-style approximation of the curvature of some performance measure at the solution. Simulations show that the true root-mean-squared angle distances between the real sources and the source estimates can be approximated well by our method. In a toy experiment, we see that we are also able to reveal the underlying grouping structure of the extracted ICA components. Furthermore, an experiment with fetal ECG data demonstrates that our approach is useful for exploratory data analysis of real-world data. (C) 2003 Elsevier B.V. All rights reserved
Y1  - 2004
SN  - 0165-1684
ER  - 
TY  - JOUR
A1  - Müller, Klaus-Robert
A1  - Vigario, R.
A1  - Meinecke, Frank C.
A1  - Ziehe, Andreas
T1  - Blind source separation techniques for decomposing event-related brain signals
N2  - Recently blind source separation (BSS) methods have been highly successful when applied to biomedical data. This paper reviews the concept of BSS and demonstrates its usefulness in the context of event-related MEG measurements. In a first experiment we apply BSS to artifact identification of raw MEG data and discuss how the quality of the resulting independent component projections can be evaluated. The second part of our study considers averaged data of event-related magnetic fields. Here, it is particularly important to monitor and thus avoid possible overfitting due to limited sample size. A stability assessment of the BSS decomposition allows to solve this task and an additional grouping of the BSS components reveals interesting structure, that could ultimately be used for gaining a better physiological modeling of the data
Y1  - 2004
SN  - 0218-1274
ER  - 
TY  - JOUR
A1  - Simons, Nadja K.
A1  - Lewinsohn, Thomas
A1  - Bluethgen, Nico
A1  - Buscot, Francois
A1  - Boch, Steffen
A1  - Daniel, Rolf
A1  - Gossner, Martin M.
A1  - Jung, Kirsten
A1  - Kaiser, Kristin
A1  - Müller, Jörg
A1  - Prati, Daniel
A1  - Renner, Swen C.
A1  - Socher, Stephanie A.
A1  - Sonnemann, Ilja
A1  - Weiner, Christiane N.
A1  - Werner, Michael
A1  - Wubet, Tesfaye
A1  - Wurst, Susanne
A1  - Weisser, Wolfgang W.
T1  - Contrasting effects of grassland management modes on species-abundance distributions of multiple groups
JF  - Agriculture, ecosystems & environment : an international journal for scientific research on the relationship of agriculture and food production to the biosphere
N2  - Intensive land use is a major cause of biodiversity loss, but most studies comparing the response of multiple taxa rely on simple diversity measures while analyses of other community attributes are only recently gaining attention. Species-abundance distributions (SADs) are a community attribute that can be used to study changes in the overall abundance structure of species groups, and whether these changes are driven by abundant or rare species. We evaluated the effect of grassland management intensity for three land-use modes (fertilization, mowing, grazing) and their combination on species richness and SADs for three belowground (arbuscular mycorrhizal fungi, prokaryotes and insect larvae) and seven aboveground groups (vascular plants, bryophytes and lichens; arthropod herbivores; arthropod pollinators; bats and birds). Three descriptors of SADs were evaluated: general shape (abundance decay rate), proportion of rare species (rarity) and proportional abundance of the commonest species (dominance). Across groups, taxonomic richness was largely unaffected by land-use intensity and only decreased with increasing mowing intensity. Of the three SAD descriptors, abundance decay rate became steeper with increasing combined land-use intensity across groups. This reflected a decrease in rarity among plants, herbivores and vertebrates. Effects of fertilization on the three descriptors were similar to the combined land-use intensity effects. Mowing intensity only affected the SAD descriptors of insect larvae and vertebrates, while grazing intensity produced a range of effects on different descriptors in distinct groups. Overall, belowground groups had more even abundance distribtitions than aboveground groups. Strong differences among aboveground groups and between above- and belowground groups indicate that no single taxonomic group can serve as an indicator for effects in other groups. In the past, the use of SADs has been hampered by concerns over theoretical models underlying specific forms of SADs. Our study shows that SAD descriptors that are not connected to a particular model are suitable to assess the effect of land use on community structure.
KW  - Biodiversity
KW  - Cutting frequency
KW  - Management intensity
KW  - Rank-abundance
KW  - Species loss
KW  - Rarity
Y1  - 2017
U6  - https://doi.org/10.1016/j.agee.2016.12.022
SN  - 0167-8809
SN  - 1873-2305
VL  - 237
SP  - 143
EP  - 153
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schall, Peter
A1  - Gossner, Martin M.
A1  - Heinrichs, Steffi
A1  - Fischer, Markus
A1  - Boch, Steffen
A1  - Prati, Daniel
A1  - Jung, Kirsten
A1  - Baumgartner, Vanessa
A1  - Blaser, Stefan
A1  - Böhm, Stefan
A1  - Buscot, Francois
A1  - Daniel, Rolf
A1  - Goldmann, Kezia
A1  - Kaiser, Kristin
A1  - Kahl, Tiemo
A1  - Lange, Markus
A1  - Müller, Jörg Hans
A1  - Overmann, Jörg
A1  - Renner, Swen C.
A1  - Schulze, Ernst-Detlef
A1  - Sikorski, Johannes
A1  - Tschapka, Marco
A1  - Türke, Manfred
A1  - Weisser, Wolfgang W.
A1  - Wemheuer, Bernd
A1  - Wubet, Tesfaye
A1  - Ammer, Christian
T1  - The impact of even-aged and uneven-aged forest management on regional biodiversity of multiple taxa in European beech forests
JF  - Journal of applied ecology : an official journal of the British Ecological Society
N2  - 1. For managed temperate forests, conservationists and policymakers favour fine-grained uneven-aged (UEA) management over more traditional coarse-grained even-aged (EA) management, based on the assumption that within-stand habitat heterogeneity enhances biodiversity. There is, however, little empirical evidence to support this assumption. We investigated for the first time how differently grained forest management systems affect the biodiversity of multiple above- and below-ground taxa across spatial scales. 2. We sampled 15 taxa of animals, plants, fungi and bacteria within the largest contiguous beech forest landscape of Germany and classified them into functional groups. Selected forest stands have been managed for more than a century at different spatial grains. The EA (coarse-grained management) and UEA (fine-grained) forests are comparable in spatial arrangement, climate and soil conditions. These were compared to forests of a nearby national park that have been unmanaged for at least 20years. We used diversity accumulation curves to compare -diversity for Hill numbers D-0 (species richness), D-1 (Shannon diversity) and D-2 (Simpson diversity) between the management systems. Beta diversity was quantified as multiple-site dissimilarity. 3. Gamma diversity was higher in EA than in UEA forests for at least one of the three Hill numbers for six taxa (up to 77%), while eight showed no difference. Only bacteria showed the opposite pattern. Higher -diversity in EA forests was also found for forest specialists and saproxylic beetles. 4. Between-stand -diversity was higher in EA than in UEA forests for one-third (all species) and half (forest specialists) of all taxa, driven by environmental heterogeneity between age-classes, while -diversity showed no directional response across taxa or for forest specialists. 5. Synthesis and applications. Comparing EA and uneven-aged forest management in Central European beech forests, our results show that a mosaic of different age-classes is more important for regional biodiversity than high within-stand heterogeneity. We suggest reconsidering the current trend of replacing even-aged management in temperate forests. Instead, the variability of stages and stand structures should be increased to promote landscape-scale biodiversity.
KW  - beta diversity
KW  - forest specialists
KW  - gamma diversity
KW  - heterogeneity
KW  - Hill numbers
KW  - saproxylic beetles
KW  - spatial grain
KW  - species accumulation curve
KW  - species richness
KW  - species turnover
Y1  - 2017
U6  - https://doi.org/10.1111/1365-2664.12950
SN  - 0021-8901
SN  - 1365-2664
VL  - 55
IS  - 1
SP  - 267
EP  - 278
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Batsios, Petros
A1  - Ishikawa-Ankerhold, Hellen Christina
A1  - Roth, Heike
A1  - Schleicher, Michael
A1  - Wong, Catherine C. L.
A1  - Müller-Taubenberger, Annette
T1  - Ate1-mediated posttranslational arginylation affects substrate adhesion and cell migration in Dictyostelium discoideum
JF  - Molecular biology of the cell : the official publication of the American Society for Cell Biology
N2  - The highly conserved enzyme arginyl-tRNA-protein transferase (Ate1) mediates arginylation, a posttranslational modification that is only incompletely understood at its molecular level. To investigate whether arginylation affects actin-dependent processes in a simple model organism, Dictyostelium discoideum, we knocked out the gene encoding Ate1 and characterized the phenotype of ate1-null cells. Visualization of actin cytoskeleton dynamics by live-cell microscopy indicated significant changes in comparison to wild-type cells. Ate1-null cells were almost completely lacking focal actin adhesion sites at the substrate-attached surface and were only weakly adhesive. In two-dimensional chemotaxis assays toward folate or cAMP, the motility of ate1-null cells was increased. However, in three-dimensional chemotaxis involving more confined conditions, the motility of ate1-null cells was significantly reduced. Live-cell imaging showed that GFP-tagged Ate1 rapidly relocates to sites of newly formed actin-rich protrusions. By mass spectrometric analysis, we identified four arginylation sites in the most abundant actin isoform of Dictyostelium, in addition to arginylation sites in other actin isoforms and several actin-binding proteins. In vitro polymerization assays with actin purified from ate1-null cells revealed a diminished polymerization capacity in comparison to wild-type actin. Our data indicate that arginylation plays a crucial role in the regulation of cytoskeletal activities.
Y1  - 2019
U6  - https://doi.org/10.1091/mbc.E18-02-0132
SN  - 1059-1524
SN  - 1939-4586
VL  - 30
IS  - 4
SP  - 453
EP  - 466
PB  - American Society for Cell Biology
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Müller, Katharina
A1  - Foerstendorf, Harald
A1  - Steudtner, Robin
A1  - Tsushima, Satoru
A1  - Kumke, Michael Uwe
A1  - Lefèvre, Grégory
A1  - Rothe, Jörg
A1  - Mason, Harris
A1  - Szabó, Zoltán
A1  - Yang, Ping
A1  - Adam, Christian K. R.
A1  - André, Rémi
A1  - Brennenstuhl, Katlen
A1  - Chiorescu, Ion
A1  - Cho, Herman M.
A1  - Creff, Gaëlle
A1  - Coppin, Frédéric
A1  - Dardenne, Kathy
A1  - Den Auwer, Christophe
A1  - Drobot, Björn
A1  - Eidner, Sascha
A1  - Hess, Nancy J.
A1  - Kaden, Peter
A1  - Kremleva, Alena
A1  - Kretzschmar, Jerome
A1  - Krüger, Sven
A1  - Platts, James A.
A1  - Panak, Petra
A1  - Polly, Robert
A1  - Powell, Brian A.
A1  - Rabung, Thomas
A1  - Redon, Roland
A1  - Reiller, Pascal E.
A1  - Rösch, Notker
A1  - Rossberg, André
A1  - Scheinost, Andreas C.
A1  - Schimmelpfennig, Bernd
A1  - Schreckenbach, Georg
A1  - Skerencak-Frech, Andrej
A1  - Sladkov, Vladimir
A1  - Solari, Pier Lorenzo
A1  - Wang, Zheming
A1  - Washton, Nancy M.
A1  - Zhang, Xiaobin
T1  - Interdisciplinary Round-Robin Test on molecular spectroscopy of the U(VI) Acetate System
JF  - ACS omega / American Chemical Society
N2  - A comprehensive molecular analysis of a simple aqueous complexing system. U(VI) acetate. selected to be independently investigated by various spectroscopic (vibrational, luminescence, X-ray absorption, and nuclear magnetic resonance spectroscopy) and quantum chemical methods was achieved by an international round-robin test (RRT). Twenty laboratories from six different countries with a focus on actinide or geochemical research participated and contributed to this scientific endeavor. The outcomes of this RRT were considered on two levels of complexity: first, within each technical discipline, conformities as well as discrepancies of the results and their sources were evaluated. The raw data from the different experimental approaches were found to be generally consistent. In particular, for complex setups such as accelerator-based X-ray absorption spectroscopy, the agreement between the raw data was high. By contrast, luminescence spectroscopic data turned out to be strongly related to the chosen acquisition parameters. Second, the potentials and limitations of coupling various spectroscopic and theoretical approaches for the comprehensive study of actinide molecular complexes were assessed. Previous spectroscopic data from the literature were revised and the benchmark data on the U(VI) acetate system provided an unambiguous molecular interpretation based on the correlation of spectroscopic and theoretical results. The multimethodologic approach and the conclusions drawn address not only important aspects of actinide spectroscopy but particularly general aspects of modern molecular analytical chemistry.
Y1  - 2019
U6  - https://doi.org/10.1021/acsomega.9b00164
SN  - 2470-1343
VL  - 4
IS  - 5
SP  - 8167
EP  - 8177
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Müller, B. R.
A1  - Cooper, R. C.
A1  - Lange, A.
A1  - Kupsch, Andreas
A1  - Wheeler, M.
A1  - Hentschel, M. P.
A1  - Staude, A.
A1  - Pandey, A.
A1  - Shyam, A.
A1  - Bruno, Giovanni
T1  - Stress-induced microcrack density evolution in beta-eucryptite ceramics
BT  - experimental observations and possible route to strain hardening
JF  - Acta materialia
N2  - In order to investigate their microcracking behaviour, the microstructures of several beta-eucryptite ceramics, obtained from glass precursor and cerammed to yield different grain sizes and microcrack densities, were characterized by laboratory and synchrotron x-ray refraction and tomography. Results were compared with those obtained from scanning electron microscopy (SEM). In SEM images, the characterized materials appeared fully dense but computed tomography showed the presence of pore clusters. Uniaxial tensile testing was performed on specimens while strain maps were recorded and analyzed by Digital Image Correlation (DIC). X-ray refraction techniques were applied on specimens before and after tensile testing to measure the amount of the internal specific surface (i.e., area per unit volume). X-ray refraction revealed that (a) the small grain size (SGS) material contained a large specific surface, originating from the grain boundaries and the interfaces of TiO2 precipitates; (b) the medium (MGS) and large grain size (LGS) materials possessed higher amounts of specific surface compared to SGS material due to microcracks, which decreased after tensile loading; (c) the precursor glass had negligible internal surface. The unexpected decrease in the internal surface of MGS and LGS after tensile testing is explained by the presence of compressive regions in the DIC strain maps and further by theoretical arguments. It is suggested that while some microcracks merge via propagation, more close mechanically, thereby explaining the observed X-ray refraction results. The mechanisms proposed would allow the development of a strain hardening route in ceramics.
KW  - Beta-eucryptite
KW  - Microcracked ceramics
KW  - X-ray refraction
KW  - Tensile load
KW  - Strain hardening
Y1  - 2017
U6  - https://doi.org/10.1016/j.actamat.2017.10.030
SN  - 1359-6454
SN  - 1873-2453
VL  - 144
SP  - 627
EP  - 641
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Müller, C.
A1  - Weber, Edzard
A1  - Gronau, Norbert
T1  - Requirements for an integration of methods analyzing social issues in knowledge organizations
Y1  - 2006
ER  - 
TY  - JOUR
A1  - Gronau, Norbert
A1  - Müller, C.
T1  - Von der Kommunikationsstrukturanalyse zur Knowledge Modeling and Description Language
Y1  - 2006
SN  - 978-3-540-28906-7
ER  -