TY  - JOUR
A1  - Böttger, Volker
A1  - Peters, L.-E.
A1  - Micheel, Burkhard
T1  - Identification of peptide mimotopes for the fluorescein hapten binding of monoclonal antibody B13-DE1
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Sellrie, Frank
A1  - Schenk, Jörg A.
A1  - Behrsing, Olaf
A1  - Bottger, Volker
A1  - Micheel, Burkhard
T1  - A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer
N2  - Mimotope peptides-peptides which mimic the binding of a hapten to its corresponding monoclonal antibody-were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope.
Y1  - 2002
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2Y-44MGNDF- 1&_coverDate=03%2F01%2F2002&_alid=268992656&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4931&_sort=d&view=c&_acct=C000053886&_v e
ER  - 
TY  - JOUR
A1  - Schenk, Jörg A.
A1  - Sellrie, Frank
A1  - Böttger, Volker
A1  - Micheel, Burkhard
A1  - Stöcklein, Walter F. M.
T1  - Generation and application of a fluorescein-specific single chain antibody
N2  - A recombinant single chain antibody fragment (designated scDE1) of the murine monoclonal anti-fluorescein antibody B13-DE1 was generated using the original hybridoma cells as source for the variable antibody heavy and light chain (VH and VL) genes. After cloning the variable genes into a phage vector a functional antibody fragment was selected by phage display panning. Recombinant antibody could be expressed as phage antibody and as soluble single chain antibody in Escherichia coli. High yield of scDE1 could also be detected in bacterial culture supernatant. The scDE1 showed the same binding specificity as the parental monoclonal antibody, i.e. it bound fluorescein, fluorescein derivatives and a fluorescein peptide mimotope. Surface plasmon resonance revealed a K(D) of 19 nM for the scDE1 compared to 0.7 nM for the monoclonal antibody. The isolated soluble scDE1 could easily be conjugated to horseradish peroxidase which allowed the use of the conjugate as universal indicator for the detection of fluorescein-labelled proteins in different immunoassays. Detection of hCG in urine was performed as a model system using scDE1. In addition to E. coli the scFv genes could also be transferred and expressed in eukaryotic cells. Finally, we generated HEK293 cells expressing the scDE1 at the cell surface.
Y1  - 2007
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRJ-4P3DY33- 1&_user=1584062&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053886&_version=1&_urlVersion=0&_userid=1584062&md5=e 4
ER  -