TY  - JOUR
A1  - Jonscher, Ernst
A1  - Flemming, Sven
A1  - Schmitt, Marius
A1  - Sabitzki, Ricarda
A1  - Reichard, Nick
A1  - Birnbaum, Jakob
A1  - Bergmann, Bärbel
A1  - Höhn, Katharina
A1  - Spielmann, Tobias
T1  - PfVPS45 Is Required for Host Cell Cytosol Uptake by Malaria Blood Stage Parasites
JF  - Cell host & microbe
N2  - During development in human erythrocytes, the malaria parasite Plasmodium falciparum internalizes a large part of the cellular content of the host cell. The internalized cytosol, consisting largely of hemoglobin, is transported to the parasite’s food vacuole where it is degraded, providing nutrients and space for growth. This host cell cytosol uptake (HCCU) is crucial for parasite survival but the parasite proteins mediating this process remain obscure. Here, we identify P. falciparum VPS45 as an essential factor in HCCU. Conditional inactivation of PfVPS45 led to an accumulation of host cell cytosol-filled vesicles within the parasite and inhibited the delivery of hemoglobin to the parasite's digestive vacuole, resulting in arrested parasite growth. A proportion of these HCCU vesicle intermediates was positive for phosphatidylinositol 3-phosphate, suggesting endosomal characteristics. Thus PfVPS45 provides insight into the elusive machinery of the ingestion pathway in a parasite that contains an endolysosomal system heavily repurposed for protein secretion.
Y1  - 2018
U6  - https://doi.org/10.1016/j.chom.2018.11.010
SN  - 1931-3128
SN  - 1934-6069
VL  - 25
IS  - 1
SP  - 166
EP  - 173
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Schwingshackl, Lukas
A1  - Ruzanska, Ulrike Alexandra
A1  - Anton, Verena
A1  - Wallroth, Raphael
A1  - Ohla, Kathrin
A1  - Knueppel, Sven
A1  - Schulze, Matthias Bernd
A1  - Pischon, Tobias
A1  - Deutschbein, Johannes
A1  - Schenk, Liane
A1  - Warschburger, Petra
A1  - Harttig, Ulrich
A1  - Boeing, Heiner
A1  - Bergmann, Manuela M.
T1  - The NutriAct Family Study: a web-based prospective study on the epidemiological, psychological and sociological basis of food choice
JF  - BMC public health
N2  - Background: Most studies on food choice have been focussing on the individual level but familial aspects may also play an important role. This paper reports of a novel study that will focus on the familial aspects of the formation of food choice among men and women aged 50-70 years by recruiting spouses and siblings (NutriAct Family Study; NFS). Discussion: Until August 4th 2017, 4783 EPIC-Participants were contacted by mail of which 446 persons recruited 2 to 5 family members (including themselves) resulting in 1032 participants, of whom 82% had started answering or already completed the questionnaires. Of the 4337 remaining EPIC-participants who had been contacted, 1040 (24%) did not respond at all, and 3297 (76%) responded but declined, in 51% of the cases because of the request to recruit at least 2 family members in the respective age range. The developed recruitment procedures and web-based methods of data collection are capable to generate the required study population including the data on individual and inter-personal determinants which will be linkable to food choice. The information on familial links among the study participants will show the role of familial traits in midlife for the adoption of food choices supporting healthy aging.
KW  - NutriAct family study
KW  - Study protocol
KW  - Food choice
KW  - Determinants
Y1  - 2018
U6  - https://doi.org/10.1186/s12889-018-5814-x
SN  - 1471-2458
VL  - 18
PB  - BMC
CY  - London
ER  -