TY  - JOUR
A1  - Meyer, Rhonda Christiane
A1  - Kusterer, Barbara
A1  - Lisec, Jan
A1  - Steinfath, Matthias
A1  - Becher, Martina
A1  - Scharr, Hanno
A1  - Melchinger, Albrecht E.
A1  - Selbig, Joachim
A1  - Schurr, Ulrich
A1  - Willmitzer, Lothar
A1  - Altmann, Thomas
T1  - QTL analysis of early stage heterosis for biomass in Arabidopsis
JF  - Theoretical and applied genetics
N2  - The main objective of this study was to identify genomic regions involved in biomass heterosis using QTL, generation means, and mode-of-inheritance classification analyses. In a modified North Carolina Design III we backcrossed 429 recombinant inbred line and 140 introgression line populations to the two parental accessions, C24 and Col-0, whose F 1 hybrid exhibited 44% heterosis for biomass. Mid-parent heterosis in the RILs ranged from −31 to 99% for dry weight and from −58 to 143% for leaf area. We detected ten genomic positions involved in biomass heterosis at an early developmental stage, individually explaining between 2.4 and 15.7% of the phenotypic variation. While overdominant gene action was prevalent in heterotic QTL, our results suggest that a combination of dominance, overdominance and epistasis is involved in biomass heterosis in this Arabidopsis cross.
KW  - Quantitative Trait Locus
KW  - recombinant inbred line
KW  - Quantitative Trait Locus analysis
KW  - dominance effect
KW  - recombinant inbred line population
Y1  - 2009
U6  - https://doi.org/10.1007/s00122-009-1074-6
SN  - 1432-2242
SN  - 0040-5752
VL  - 129
IS  - 2
SP  - 227
EP  - 237
PB  - Springer Nature
CY  - Berlin
ER  - 
TY  - GEN
A1  - Meyer, Rhonda Christiane
A1  - Kusterer, Barbara
A1  - Lisec, Jan
A1  - Steinfath, Matthias
A1  - Becher, Martina
A1  - Scharr, Hanno
A1  - Melchinger, Albrecht E.
A1  - Selbig, Joachim
A1  - Schurr, Ulrich
A1  - Willmitzer, Lothar
A1  - Altmann, Thomas
T1  - QTL analysis of early stage heterosis for biomass in Arabidopsis
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The main objective of this study was to identify genomic regions involved in biomass heterosis using QTL, generation means, and mode-of-inheritance classification analyses. In a modified North Carolina Design III we backcrossed 429 recombinant inbred line and 140 introgression line populations to the two parental accessions, C24 and Col-0, whose F 1 hybrid exhibited 44% heterosis for biomass. Mid-parent heterosis in the RILs ranged from −31 to 99% for dry weight and from −58 to 143% for leaf area. We detected ten genomic positions involved in biomass heterosis at an early developmental stage, individually explaining between 2.4 and 15.7% of the phenotypic variation. While overdominant gene action was prevalent in heterotic QTL, our results suggest that a combination of dominance, overdominance and epistasis is involved in biomass heterosis in this Arabidopsis cross.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1330 
KW  - Quantitative Trait Locus
KW  - recombinant inbred line
KW  - Quantitative Trait Locus analysis
KW  - dominance effect
KW  - recombinant inbred line population
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-431272
SN  - 1866-8372
IS  - 1330
ER  - 
TY  - JOUR
A1  - Lisec, Jan
A1  - Steinfath, Matthias
A1  - Meyer, Rhonda C.
A1  - Selbig, Joachim
A1  - Melchinger, Albrecht E.
A1  - Willmitzer, Lothar
A1  - Altmann, Thomas
T1  - Identification of heterotic metabolite QTL in Arabidopsis thaliana RIL and IL populations
N2  - Two mapping populations of a cross between the Arabidopsis thaliana accessions Col-0 and C24 were cultivated and analyzed with respect to the levels of 181 metabolites to elucidate the biological phenomenon of heterosis at the metabolic level. The relative mid-parent heterosis in the F-1 hybrids was <20% for most metabolic traits. The first mapping population consisting of 369 recombinant inbred lines (RILs) and their test cross progeny with both parents allowed us to determine the position and effect of 147 quantitative trait loci (QTL) for metabolite absolute mid-parent heterosis (aMPH). Furthermore, we identified 153 and 83 QTL for augmented additive (Z(1)) and dominance effects (Z(2)), respectively. We identified putative candidate genes for these QTL using the ARACYC database (http://www.arabidopsis.org/ biocyc), and calculated the average degree of dominance, which was within the dominance and over-dominance range for most metabolites. Analyzing a second population of 41 introgression lines (ILs) and their test crosses with the recurrent parent, we identified 634 significant differences in metabolite levels. Nine per cent of these effects were classified as over-dominant, according to the mode of inheritance. A comparison of both approaches suggested epistasis as a major contributor to metabolite heterosis in Arabidopsis. A linear combination of metabolite levels was shown to significantly correlate with biomass heterosis (r = 0.62).
Y1  - 2009
UR  - http://www3.interscience.wiley.com/cgi-bin/issn?DESCRIPTOR=PRINTISSN&VALUE=0960-7412
U6  - https://doi.org/10.1111/j.1365-313X.2009.03910.x
SN  - 0960-7412
ER  -