TY  - JOUR
A1  - Haase, A.
A1  - Mantion, A.
A1  - Graf, P.
A1  - Plendl, J.
A1  - Thünemann, Andreas F.
A1  - Meier, Wolfgang P.
A1  - Taubert, Andreas
A1  - Luch, A.
T1  - A novel type of silver nanoparticles and their advantages in toxicity testing in cell culture systems
JF  - Archives of toxicology : official journal of EUROTOX
N2  - Silver nanoparticles (SNPs) are among the most commercialized nanoparticles worldwide. Often SNP are used because of their antibacterial properties. Besides that they possess unique optic and catalytic features, making them highly interesting for the creation of novel and advanced functional materials. Despite its widespread use only little data exist in terms of possible adverse effects of SNP on human health. Conventional synthesis routes usually yield products of varying quality and property. It thus may become puzzling to compare biological data from different studies due to the great variety in sizes, coatings or shapes of the particles applied. Here, we applied a novel synthesis approach to obtain SNP of well-defined colloidal and structural properties. Being stabilized by a covalently linked small peptide, these particles are nicely homogenous, with narrow size distribution, and form monodisperse suspensions in aqueous solutions. We applied these peptide-coated SNP in two different sizes of 20 or 40 nm (Ag20Pep and Ag40Pep) and analyzed responses of THP-1-derived human macrophages while being exposed against these particles. Gold nanoparticles of similar size and coating (Au20Pep) were used for comparison. The cytotoxicity of particles was assessed by WST-1 and LDH assays, and the uptake into the cells was confirmed via transmission electron microscopy. In summary, our data demonstrate that this novel type of SNP is well suited to serve as model system for nanoparticles to be tested in toxicological studies in vitro.
KW  - Silver nanoparticles
KW  - Peptide coating
KW  - Nanotoxicity
Y1  - 2012
U6  - https://doi.org/10.1007/s00204-012-0836-0
SN  - 0340-5761
VL  - 86
IS  - 7
SP  - 1089
EP  - 1098
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Tentschert, J.
A1  - Draude, F.
A1  - Jungnickel, H.
A1  - Haase, A.
A1  - Mantion, Alexandre
A1  - Galla, S.
A1  - Thuenemann, Andreas F.
A1  - Taubert, Andreas
A1  - Luch, A.
A1  - Arlinghaus, H. F.
T1  - TOF-SIMS analysis of cell membrane changes in functional impaired human macrophages upon nanosilver treatment
JF  - Surface and interface analysis : an international journal devoted to the development and application of techniques for the analysis surfaces, interfaces and thin films
N2  - Silver nanoparticles (SNP) are among the most commercialized nanoparticles. Here, we show that peptide-coated SNP cause functional impairment of human macrophages. A dose-dependent inhibition of phagocytosis is observed after nanoparticle treatment, and pretreatment of cells with N-acetyl cysteine (NAC) can counteract the phagocytosis disturbances caused by SNP.
 Using the surface-sensitive mode of time-of-flight secondary ion mass spectrometry, in combination with multivariate statistical methods, we studied the composition of cell membranes in human macrophages upon exposure to SNP with and without NAC preconditioning. This method revealed characteristic changes in the lipid pattern of the cellular membrane outer leaflet in those cells challenged by SNP. Statistical analyses resulted in 19 characteristic ions, which can be used to distinguish between NAC pretreated and untreated macrophages. The present study discusses the assignments of surface cell membrane phospholipids for the identified ions and the resulting changes in the phospholipid pattern of treated cells. We conclude that the adverse effects in human macrophages caused by SNP can be partially reversed through NAC administration. Some alterations, however, remained.
KW  - silver nanoparticles
KW  - lipidomics
KW  - N-acetyl cysteine
KW  - phagocytosis
KW  - oxidative stress
Y1  - 2013
U6  - https://doi.org/10.1002/sia.5155
SN  - 0142-2421
VL  - 45
IS  - 1
SP  - 483
EP  - 485
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Krohn, Arne
A1  - Materne, Stefanie
A1  - Schlutow, A.
A1  - Wilhelm, R.
A1  - Metzdorf, R.
A1  - Wilk, B.
A1  - Förster, B.
A1  - Schade, Bernd
A1  - Kitzig, Angelika
A1  - Müller, Jörg
A1  - Haase, Walter
A1  - Rückert-John, Jana
T1  - Agenda 21-Prozesse für zukunftsfähige Kommunen in Brandenburg
N2  - Agenda 21-Prozesse für zukunftsfähige Kommunen in Brandenburg KROHN, A.: Stadtentwicklung und Lokale Agenda 21 – Zwei Seiten einer Medaille ; MATERNE, S.: Agenda 21 in Oranienburg – die Entwicklung eines Leitbildes ; SCHLUTOW, A.; WILHELM, B.; METZDORF, R.; WILK, B., FÖRSTER, B.: Interessengemeinschaft "Ökologie 2000 - Unternehmer für die Umwelt" – Anstoß der Wirtschaft für eine lokale Agenda 21 in Strausberg ; SCHADE, B.: Agenda 21 im Landkreis Potsdam-Mittelmark – Rahmen für lokale Aktivitäten ; KITZIG, A.: Potsdam, Stadt der Toleranz – unterwegs mit Geschichts- und Verantwortungsbewußtsein für die Zukunft. Die Lokale Agenda 21 ; MÜLLER, J.: Umsetzung eines Klimaschutzkonzeptes – Schritte zu einer nachhaltigen Entwicklung der Stadt Eberswalde , HAASE, W.: Eine lokale Agenda 21 für Kleinmachnow , RÜCKERT-JOHN, J.: Auf dem Weg zur Nachhaltigkeit. Ergebnisse einer Dorfstudie
Y1  - 1999
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-4120
ER  - 
TY  - JOUR
A1  - Draude, F.
A1  - Galla, S.
A1  - Pelster, Axel
A1  - Tentschert, J.
A1  - Jungnickel, H.
A1  - Haase, Alfred
A1  - Mantion, Alexandre
A1  - Thuenemann, Andreas F.
A1  - Taubert, Andreas
A1  - Luch, A.
A1  - Arlinghaus, H. F.
T1  - ToF-SIMS and Laser-SNMS analysis of macrophages after exposure to silver nanoparticles
JF  - Surface and interface analysis : an international journal devoted to the development and application of techniques for the analysis surfaces, interfaces and thin films
N2  - Silver nanoparticles (SNPs) are among the most commercialized nanoparticles because of their antibacterial effects. Besides being employed, e. g. as a coatingmaterial for sterile surfaces in household articles and appliances, the particles are also used in a broad range of medical applications. Their antibacterial properties make SNPs especially useful for wound disinfection or as a coating material for prostheses and surgical instruments. Because of their optical characteristics, the particles are of increasing interest in biodetection as well. Despite the widespread use of SNPs, there is little knowledge of their toxicity. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and laser post-ionization secondary neutral mass spectrometry (Laser-SNMS) were used to investigate the effects of SNPs on human macrophages derived from THP-1 cells in vitro. For this purpose, macrophages were exposed to SNPs. The SNP concentration ranges were chosen with regard to functional impairments of the macrophages. To optimize the analysis of the macrophages, a special silicon wafer sandwich preparation technique was employed; ToF-SIMS was employed to characterize fragments originating from macrophage cell membranes. With the use of this optimized sample preparation method, the SNP-exposed macrophages were analyzed with ToF-SIMS and with Laser-SNMS. With Laser-SNMS, the three-dimensional distribution of SNPs in cells could be readily detected with very high efficiency, sensitivity, and submicron lateral resolution. We found an accumulation of SNPs directly beneath the cell membrane in a nanoparticular state as well as agglomerations of SNPs inside the cells.
KW  - Laser-SNMS
KW  - ToF-SIMS
KW  - life sciences
KW  - imaging
KW  - nanoparticles
KW  - three-dimensional depth profiling
Y1  - 2013
U6  - https://doi.org/10.1002/sia.4902
SN  - 0142-2421
VL  - 45
IS  - 1
SP  - 286
EP  - 289
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Lutze, Jana
A1  - Bañares, Miguel A.
A1  - Pita, Marcos
A1  - Haase, Andrea
A1  - Luch, Andreas
A1  - Taubert, Andreas
T1  - alpha-((4-Cyanobenzoyl)oxy)-omega-methyl poly(ethylene glycol)
BT  - a new stabilizer for silver nanoparticles
JF  - Beilstein journal of nanotechnology
N2  - The article describes the synthesis and properties of alpha-((4-cyanobenzoyl)oxy)-omega-methyl poly(ethylene glycol), the first poly(ethylene glycol) stabilizer for metal nanoparticles that is based on a cyano rather than a thiol or thiolate anchor group. The silver particles used to evaluate the effectiveness of the new stabilizer typically have a bimodal size distribution with hydrodynamic diameters of ca. 13 and ca. 79 nm. Polymer stability was evaluated as a function of the pH value both for the free stabilizer and for the polymers bound to the surface of the silver nanoparticles using H-1 NMR spectroscopy and zeta potential measurements. The polymer shows a high stability between pH 3 and 9. At pH 12 and higher the polymer coating is degraded over time suggesting that alpha-((4-cyanobenzoyl) oxy)-omega-methyl poly(ethylene glycol) is a good stabilizer for metal nanoparticles in aqueous media unless very high pH conditions are present in the system. The study thus demonstrates that cyano groups can be viable alternatives to the more conventional thiol/thiolate anchors.
KW  - cyano anchor group
KW  - poly(ethylene glycol)
KW  - polymer coating
KW  - silver nanoparticles
Y1  - 2017
U6  - https://doi.org/10.3762/bjnano.8.67
SN  - 2190-4286
VL  - 8
SP  - 627
EP  - 635
PB  - Beilstein-Institut zur Förderung der Chemischen Wissenschaften
CY  - Frankfurt, Main
ER  -