TY  - JOUR
A1  - Brechun, Katherine Emily
A1  - Arndt, Katja Maren
A1  - Woolley, G. Andrew
T1  - Selection of protein-protein interactions of desired affinities with a bandpass circuit
JF  - Journal of molecular biology : JMB
N2  - We have developed a genetic circuit in Escherichia coli that can be used to select for protein-protein interactions of different strengths by changing antibiotic concentrations in the media. The genetic circuit links protein-protein interaction strength to beta-lactamase activity while simultaneously imposing tuneable positive and negative selection pressure for beta-lactamase activity. Cells only survive if they express interacting proteins with affinities that fall within set high- and low-pass thresholds; i.e. the circuit therefore acts as a bandpass filter for protein-protein interactions. We show that the circuit can be used to recover protein-protein interactions of desired affinity from a mixed population with a range of affinities. The circuit can also be used to select for inhibitors of protein-protein interactions of defined strength. (C) 2018 Elsevier Ltd. All rights reserved.
KW  - synthetic biology
KW  - genetic circuit
KW  - biological engineering
KW  - protein-protein interactions
KW  - twin-arginine translocation
KW  - selection system
Y1  - 2018
U6  - https://doi.org/10.1016/j.jmb.2018.11.011
SN  - 0022-2836
SN  - 1089-8638
VL  - 431
IS  - 2
SP  - 391
EP  - 400
PB  - Elsevier
CY  - London
ER  - 
TY  - JOUR
A1  - Brechun, Katherine E.
A1  - Arndt, Katja Maren
A1  - Woolley, G. Andrew
T1  - Strategies for the photo-control of endogenous protein activity
JF  - Current opinion in structural biology : review of all advances ; evaluation of key references ; comprehensive listing of papers
Y1  - 2017
U6  - https://doi.org/10.1016/j.sbi.2016.11.014
SN  - 0959-440X
SN  - 1879-033X
VL  - 45
SP  - 53
EP  - 58
PB  - Elsevier
CY  - London
ER  - 
TY  - JOUR
A1  - Zhang, Fuzhong Z.
A1  - Timm, Katharina A.
A1  - Arndt, Katja Maren
A1  - Woolley, G. Andrew
T1  - Photocontrol of Coiled-Coil Proteins in Living Cells
N2  - Light switching of the activity of a coiled-coil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzene-cross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/26737/
U6  - https://doi.org/10.1002/anie.201000909
SN  - 1433-7851
ER  - 
TY  - JOUR
A1  - Mazumder, Mostafizur
A1  - Brechun, Katherine E.
A1  - Kim, Yongjoo B.
A1  - Hoffmann, Stefan A.
A1  - Chen, Yih Yang
A1  - Keiski, Carrie-Lynn
A1  - Arndt, Katja Maren
A1  - McMillen, David R.
A1  - Woolley, G. Andrew
T1  - An Escherichia coli system for evolving improved light-controlled DNA-binding proteins
JF  - Protein engineering design & selection
N2  - Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used in other areas of protein engineering. Here we report an approach for screening light-switchable DNA-binding proteins that relies on light-dependent repression of the transcription of a fluorescent reporter. We demonstrate that the method can be used to recover a known light-switchable DNA-binding protein from a random library.
KW  - directed evolution
KW  - fluorescent reporter
KW  - optogenetics
Y1  - 2015
U6  - https://doi.org/10.1093/protein/gzv033
SN  - 1741-0126
SN  - 1741-0134
VL  - 28
IS  - 9
SP  - 293
EP  - 302
PB  - Oxford Univ. Press
CY  - Oxford
ER  -