TY - GEN A1 - Rodríguez Sillke, Yasmina A1 - Schumann, Michael A1 - Lissner, Donata A1 - Branchi, Frederica A1 - Glauben, Rainer A1 - Siegmund, Britta T1 - Small intestinal inflammation but not colitis drives pro-inflammatory nutritional antigen-specific T-cell response T2 - Journal of Crohn's and Colitis N2 - Background: Inflammatory bowel disease (IBD) represents a dysregulation of the mucosal immune system. The pathogenesis of Crohn’s disease (CD) and ulcerative colitis (UC) is linked to the loss of intestinal tolerance and barrier function. The healthy mucosal immune system has previously been shown to be inert against food antigens. Since the small intestine is the main contact surface for antigens and therefore the immunological response, the present study served to analyse food-antigen-specific T cells in the peripheral blood of IBD patients. Methods: Peripheral blood mononuclear cells of CD, with an affected small intestine, and UC (colitis) patients, either active or in remission, were stimulated with the following food antigens: gluten, soybean, peanut and ovalbumin. Healthy controls and celiac disease patients were included as controls. Antigen-activated CD4+ T cells in the peripheral blood were analysed by a magnetic enrichment of CD154+ effector T cells and a cytometric antigen-reactive T-cell analysis (‘ARTE’ technology) followed by characterisation of the ef- fector response. Results: The effector T-cell response of antigen-specific T cells were compared between CD with small intestinal inflammation and UC where inflammation was restricted to the colon. Among all tested food antigens, the highest frequency of antigen-specific T cells (CD4+CD154+) was found for gluten. Celiac disease patients were included as control, since gluten has been identified as the disease- causing antigen. The highest frequency of gluten antigen-specific T cells was revealed in active CD when compared with UC, celiac disease on a gluten-free diet (GFD) and healthy controls. Ovalbuminspecific T cells were almost undetectable, whereas the reaction to soybean and peanut was slightly higher. But again, the strong- est reaction was observed in CD with small intestinal involvement compared with UC. Remarkably, in celiac disease on a GFD only antigen-specific cells for gluten were detected. These gluten-specific T cells were characterised by up-regulation of the pro-inflammatory cytokines IFN-γ, IL-17A and TNF-α. IFN-g was exclusively elevated in CD patients with active disease. Gluten-specific T-cells expressing IL-17A were increased in all IBD patients. Furthermore, T cells of CD patients, independent of disease activity, revealed a high expression of the pro-inflammatory cytokine TNF-α. Conclusion: The ‘ARTE’-technique allows to analyse and quantify food antigen specific T cells in the peripheral blood of IBD patients indicating a potential therapeutic insight. These data provide evidence that small intestinal inflammation in CD is key for the development of a systemic pro-inflammatory effector T-cell response driven by food antigens. Y1 - 2020 U6 - https://doi.org/10.1093/ecco-jcc/jjz203.172 SN - 1873-9946 SN - 1876-4479 VL - 14 SP - S154 EP - S155 PB - Oxford Univ. Press CY - Oxford ER - TY - GEN A1 - Döge, Nadine A1 - Schumacher, Fabian A1 - Balzus, Benjamin A1 - Colombo, Miriam A1 - Hadam, Sabrina A1 - Rancan, Fiorenza A1 - Blume-Peytavi, Ulrike A1 - Kleuser, Burkhard A1 - Bodmeier, Roland A1 - Vogt, Annika T1 - Particle- based formulations and controlled skin barrier disruption have a signifi cant impact on the delivery and penetration kinetics of dexamethasone as assessed in an ex vivo microdialysis T2 - Journal der Deutschen Dermatologischen Gesellschaft N2 - Preclinical assessment of penetration not only in intact, but also in barrier‐disrupted skin is important to explore the surplus value of novel drug delivery systems, which can be specifically designed for diseased skin. Here, we characterized physical and chemical barrier disruption protocols for short‐term ex vivo skin cultures with regard to structural integrity, physiological and biological parameters. Further, we compared the penetration of dexamethasone (Dex) in different nanoparticle‐based formulations in stratum corneum, epidermis and dermis extracts of intact vs. barrier‐disrupted skin as well as by dermal microdialysis at 6, 12 and 24 hours after topical application. Dex was quantified by liquid‐chromatography ‐ tandem‐mass spectrometry (LC‐MS/MS). Simultaneously, we investigated the Dex efficacy by interleukin (IL) analysis. Tape‐stripping (TS) and 4 hours sodium lauryl sulfate 5 % (SLS) exposure were identified as highly effective barrier disruption methods assessed by reproducible transepidermal water loss (TEWL) changes and IL‐6/8 increase which was more pronounced in SLS‐treated skin. The barrier state has also a significant impact on the Dex penetration kinetics: for all formulations, TS highly increased dermal Dex concentration despite the fact that nanocrystals quickly and effectively penetrated both, intact and barrier‐disrupted skin reaching significantly higher dermal Dex concentration after 6 hours compared to Dex cream. The surplus value of encapsulation in ethyl cellulose nanocarriers could mostly be observed when applied on intact skin, in general showing a delayed Dex penetration. Estimation of cytokines was limited due to the trauma caused by probe insertion. In summary, ex vivo human skin is a highly interesting short‐term preclinical model for the analysis of penetration and efficacy of novel drug delivery systems. Y1 - 2017 SN - 1610-0379 SN - 1610-0387 VL - 15 SP - 182 EP - 182 PB - Wiley CY - Berlin ER - TY - GEN A1 - Halibasic, Emina A1 - Fuerst, Elisabeth A1 - Heiden, Denis A1 - Japtok, Lukasz A1 - Diesner, Susanne C. A1 - Hillebrand, P. A1 - Trauner, Michael A1 - Kleuser, Burkhard A1 - Kazemi-Shirazi, Lili A1 - Untersmayr, Eva T1 - Significantly reduced plasma levels of the bioactive sphingolipid S1P in lung transplanted cystic fibrosis patients are associated with gastrointestinal symptoms T2 - Allergy Y1 - 2017 SN - 0105-4538 SN - 1398-9995 VL - 72 IS - S103 SP - 195 EP - 195 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Chen, Pan A1 - Bornhorst, Julia A1 - Neely, M. Diana A1 - Avila, Daiana Silva T1 - Mechanisms and Disease Pathogenesis Underlying Metal-Induced Oxidative Stress T2 - Oxidative Medicine and Cellular Longevity Y1 - 2018 U6 - https://doi.org/10.1155/2018/7612172 SN - 1942-0900 SN - 1942-0994 PB - Hindawi CY - London ER - TY - GEN A1 - Steinberg, Pablo T1 - Only one Component of a holistic Nutrition Policy T1 - Nur ein Baustein einer ganzheitlichen Ernährungspolitik T2 - Fleischwirtschaft Y1 - 2018 SN - 0015-363X VL - 98 IS - 11 SP - 8 EP - 9 PB - Deutscher Fachverlag GmbH CY - Frankfurt am Main ER - TY - GEN A1 - Hocher, Berthold A1 - Zeng, Shufei T1 - Need for better PTH assays for clinical research and patient treatment T2 - Clinical chemistry and laboratory medicine : journal of the Forum of the European Societies of Clinical Chemistry - the European Branch of the International Federation of Clinical Chemistry and Laboratory Medicine Y1 - 2017 U6 - https://doi.org/10.1515/cclm-2017-0617 SN - 1434-6621 SN - 1437-4331 VL - 56 IS - 2 SP - 183 EP - 185 PB - De Gruyter CY - Berlin ER - TY - GEN A1 - Pischon, Hannah A1 - Radbruch, Moritz A1 - Ostrowski, Anja A1 - Schumacher, Fabian A1 - Hoenzke, Stefan A1 - Kleuser, Burkhard A1 - Hedtrich, Sarah A1 - Fluhr, Joachim W. A1 - Gruber, Achim D. A1 - Mundhenk, Lars T1 - How Effective Is Tacrolimus in the Imiquimod BT - Induced Mouse Model of Psoriasis? T2 - The journal of investigative dermatology Y1 - 2017 U6 - https://doi.org/10.1016/j.jid.2017.09.019 SN - 0022-202X SN - 1523-1747 VL - 138 IS - 2 SP - 455 EP - 458 PB - Elsevier CY - New York ER - TY - GEN A1 - Honnen, S. A1 - Wellenberg, Anna A1 - Weides, L. A1 - Bornhorst, Julia A1 - Crone, B. A1 - Karst, U. A1 - Fritz, G. T1 - Identification of potent drug candidates for the prevention of cisplatin-induced neurotoxicity in the model organism C. elegans T2 - Naunyn-Schmiedeberg's archives of pharmacology Y1 - 2018 UR - https://link.springer.com/content/pdf/10.1007/s00210-018-1477-5.pdf U6 - https://doi.org/10.1007/s00210-018-1477-5 SN - 0028-1298 SN - 1432-1912 VL - 391 SP - S4 EP - S4 PB - Springer CY - New York ER - TY - GEN A1 - Reichetzeder, Christoph A1 - Hocher, Berthold T1 - DPP4 inhibition prevents AKI T2 - Oncotarget KW - acute kidney injury KW - DPP-4 inhibitors KW - ischemia reperfusion injury KW - gliptins KW - Dipeptidyl peptidase IV Y1 - 2017 U6 - https://doi.org/10.18632/oncotarget.20212 SN - 1949-2553 VL - 8 SP - 64655 EP - 64656 PB - Impact Journals LLC CY - Orchard Park ER - TY - GEN A1 - Hocher, Berthold A1 - Zeng, Shufei T1 - Clear the fog around parathyroid hormone assays BT - what do iPTH assays really measure? T2 - Clinical journal of the American Society of Nephrology KW - Assays KW - Biological Assay KW - CKD KW - oxidative stress KW - PTH Y1 - 2018 U6 - https://doi.org/10.2215/CJN.01730218 SN - 1555-9041 SN - 1555-905X VL - 13 IS - 4 SP - 524 EP - 526 PB - American Society of Nephrology CY - Washington ER - TY - GEN A1 - Fernando, Raquel A1 - Drescher, Cathleen A1 - Deubel, Stefanie A1 - Grune, Tilman A1 - Castro, Jose Pedro T1 - Distinct proteasomal activity for fast and slow twitch skeletal muscle during aging T2 - Free radical biology and medicine : the official journal of the Oxygen Society, a constituent member of the International Society for Free Radical Research N2 - Skeletal muscle alterations during aging lead to dysfunctional metabolism, correlating with frailty and early mortality. The loss of proteostasis is a hallmark of aging. Whether proteostasis loss plays a role in muscle aging remains elusive. To address this question we collected muscles, Soleus (SOL, type I) and Extensor digitorum longus (EDL, type II), from young (4 months) and old (25 months) C57BL/6 mice and evaluated the proteasomal system. Initial work showed decreased 26 S activity in old SOL. EDL displayed lower proteasomal activity in both ages compared to any of the SOL ages. Moreover, in order to understand if during aging there is the so-called “fiber switch from fast-to-slow”, we performed western blots against sMHC and fMHC (slow and fast myosin heavy chain, respectively). Preliminary results suggest that young SOL is composed by slow twitch fibers but also contains fast twitch fibers, while young EDL seems to be mostly composed by fast twitch fibers that level down during aging, suggesting the switch. As a conclusion, EDL seems to have less proteasomal activity, however, if this is a contributor or a consequence to the muscle fiber switch during aging still needs further investigation. Y1 - 2018 U6 - https://doi.org/10.1016/j.freeradbiomed.2018.04.393 SN - 0891-5849 SN - 1873-4596 VL - 120 SP - S119 EP - S119 PB - Elsevier CY - New York ER - TY - GEN A1 - Schulze, Matthias Bernd A1 - Martinez-Gonzalez, Miguel A. A1 - Fung, Teresa T. A1 - Lichtenstein, Alice H. A1 - Forouhi, Nita G. T1 - Food based dietary patterns and chronic disease prevention T2 - BMJ-British medical journal N2 - Matthias B Schulze and colleagues discuss current knowledge on the associations between dietary patterns and cancer, coronary heart disease, stroke, and type 2 diabetes, focusing on areas of uncertainty and future research directions. Y1 - 2018 U6 - https://doi.org/10.1136/bmj.k2396 SN - 1756-1833 VL - 361 PB - BMJ Publishing Group CY - London ER - TY - GEN A1 - Jamnok, Jutatip A1 - Sanchaisuriya, Kanokwan A1 - Yamsri, Supawadee A1 - Fucharoen, Goonnapa A1 - Fucharoen, Supan A1 - Schweigert, Florian J. A1 - Sanchaisuriya, Pattara T1 - Application of a new portable nephelometer for screening thalassemia in countries with limited resources T2 - International Journal of Laboratory Hematology Y1 - 2018 SN - 1751-5521 SN - 1751-553X VL - 40 SP - 62 EP - 62 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Jamnok, Jutatip A1 - Sanchaisuriya, Kanokwan A1 - Yamsri, Supawadee A1 - Fucharoen, Goonnapa A1 - Fucharoen, Supan A1 - Schweigert, Florian J. A1 - Sanchaisuriya, Pattara T1 - Application of a new portable nephelometer for screening thalassemia in countries with limited resources T2 - International journal of laboratory hematology N2 - One-tube osmotic fragility (OF) test is a rapid test used widely for screening thalassemia in countries with limited resources. The test has important limitation in that its accuracy relies on observers’ experience. The iCheck Turbidity is a prototype of portable nephelometer developed by BioAnalyt (Bioanalyt GmbH, Germany). In this study, we assessed the applicability of the iCheck Turbidity, for checking turbidity of the OF-test Y1 - 2018 SN - 1751-5521 SN - 1751-553X VL - 40 SP - 62 EP - 62 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Kleuser, Burkhard T1 - The enigma of sphingolipids in health and disease T2 - International journal of molecular sciences Y1 - 2018 U6 - https://doi.org/10.3390/ijms19103126 SN - 1422-0067 VL - 19 IS - 10 PB - MDPI CY - Basel ER - TY - GEN A1 - Henze, Andrea T1 - Proteinoxidation als Indikator des Alterungsphänotyps und Target einer individualisierten Ernährungsintervention (ProAID) T1 - Protein Oxidation as an Indicator of the Aging Phenotype and Target of an individualized Nutritional Intervention (ProAID) T2 - Ernährungs-Umschau : Forschung & Praxis N2 - Oxidative posttranslationale Modifikationen endogener Proteine werden v. a. durch reaktive Sauerstoff- und Stickstoffspezies (engl:. Reactive Oxygen Species, ROS, reactive nitrogen species, RNS) hervorgerufen und können sowohl reversibel (z. B. Disulfidbindungen) als auch irreversibel (z. B. Proteincarbonyle) erfolgen [1–3]. Lange wurde angenommen, dass oxidative posttranslationale Proteinmodifikationen (oxPTPM) nur von untergeordneter Bedeutung für den Metabolismus sind. Tatsächlich handelt es sich jedoch um einen physiologischen Prozess, der über die Modulation der Proteinstruktur auch die Proteinfunktion (z. B. Enzymaktivität, Stabilität) und somit zahlreiche Stoffwechselwege wie den Energiestoffwechsel, die Immunfunktion, die vaskuläre Funktion sowie Apoptose und Genexpression beeinflussen kann. Die Bildung von oxPTPM ist dabei hochreguliert und hängt u. a. von der Proteinstruktur, der Verfügbarkeit von ROS und RNS sowie dem lokalen Mikromilieu der Zelle ab [2, 4]. Y1 - 2018 SN - 0174-0008 VL - 65 IS - 10 SP - M566 EP - M567 PB - Umschau-Zeitschriftenverl. CY - Frankfurt, Main ER - TY - GEN A1 - Uhlig, Katja A1 - Gehre, Christian P. A1 - Kammerer, Sarah A1 - Küpper, Jan-Heiner A1 - Coleman, Charles Dominic A1 - Püschel, Gerhard Paul A1 - Duschl, Claus T1 - Real-time monitoring of oxygen consumption of hepatocytes in a microbioreactor T2 - Toxicology letters Y1 - 2018 U6 - https://doi.org/10.1016/j.toxlet.2018.06.652 SN - 0378-4274 SN - 1879-3169 VL - 295 SP - S115 EP - S115 PB - Elsevier CY - Clare ER - TY - GEN A1 - Ponce, Carol Barahona A1 - Scherer, Dominique A1 - Boekstegers, Felix A1 - Garate-Calderon, Valentina A1 - Jenab, Mazda A1 - Aleksandrova, Krasimira A1 - Katzke, Verena A1 - Weiderpass, Elisabete A1 - Bonet, Catalina A1 - Moradi, Tahereh A1 - Fischer, Krista A1 - Bossers, Willem A1 - Brenner, Hermann A1 - Schöttker, Ben A1 - Holleczek, Bernd A1 - Hveem, Kristian A1 - Eklund, Niina A1 - Voelker, Uwe A1 - Waldenberger, Melanie A1 - Bermejo, Justo Lorenzo T1 - Arsenic and gallbladder cancer risk BT - Mendelian randomization analysis of European prospective data T2 - International journal of cancer KW - arsenic KW - gallbladder cancer KW - Mendelian randomization Y1 - 2019 U6 - https://doi.org/10.1002/ijc.32837 SN - 0020-7136 SN - 1097-0215 VL - 146 IS - 9 SP - 2648 EP - 2650 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Wellenberg, Anna A1 - Weides, L. A1 - Bornhorst, Julia A1 - Crone, Barbara A1 - Karst, U. A1 - Fritz, G. A1 - Honnen, S. T1 - Molecular and electrophysiological analysis of platinum-induced neurotoxicity using the model organism C. elegans T2 - Naunyn-Schmiedeberg's archives of pharmacology Y1 - 2019 UR - https://link.springer.com/content/pdf/10.1007%2Fs00210-019-01621-6.pdf U6 - https://doi.org/10.1007/s00210-019-01621-6 SN - 0028-1298 SN - 1432-1912 VL - 392 SP - S63 EP - S63 PB - Springer CY - New York ER - TY - GEN A1 - Kleuser, Burkhard T1 - Medikamentennebenwirkungen auf Haut und Schleimhaut – allergische oder pharmakologisch erklärbare Reaktionen T2 - Allergologie T2 - Side Effects on Skin and Mucous Membrane - allergic or pharmacologically explainable Reactions Y1 - 2017 SN - 0344-5062 VL - 40 IS - 10 SP - 420 EP - 421 PB - Dustri-Verlag CY - Deisenhofen-München ER -