TY - JOUR A1 - González-Fortes, Gloria M. A1 - Jones, Eppie R. A1 - Lightfoot, Emma A1 - Bonsall, Clive A1 - Lazar, Catalin A1 - Dolores Garralda, Maria A1 - Drak, Labib A1 - Siska, Veronika A1 - Simalcsik, Angela A1 - Boroneant, Adina A1 - Vidal Romani, Juan Ramon A1 - Vaqueiro Rodriguez, Marcos A1 - Arias, Pablo A1 - Pinhasi, Ron A1 - Manica, Andrea A1 - Hofreiter, Michael T1 - Paleogenomic Evidence for Multi-generational Mixing between Neolithic Farmers and Mesolithic Hunter-Gatherers in the Lower Danube Basin JF - Current biology N2 - The transition from hunting and gathering to farming involved profound cultural and technological changes. In Western and Central Europe, these changes occurred rapidly and synchronously after the arrival of early farmers of Anatolian origin [1-3], who largely replaced the local Mesolithic hunter-gatherers [1, 4-6]. Further east, in the Baltic region, the transition was gradual, with little or no genetic input from incoming farmers [7]. Here we use ancient DNA to investigate the relationship between hunter-gatherers and farmers in the Lower Danube basin, a geographically intermediate area that is characterized by a rapid Neolithic transition but also by the presence of archaeological evidence that points to cultural exchange, and thus possible admixture, between hunter-gatherers and farmers. We recovered four human paleogenomes (1.13 to 4.13 coverage) from Romania spanning a time transect between 8.8 thousand years ago (kya) and 5.4 kya and supplemented them with two Mesolithic genomes (1.73- and 5.33) from Spain to provide further context on the genetic background of Mesolithic Europe. Our results show major Western hunter-gatherer (WHG) ancestry in a Romanian Eneolithic sample with a minor, but sizeable, contribution from Anatolian farmers, suggesting multiple admixture events between hunter-gatherers and farmers. Dietary stableisotope analysis of this sample suggests a mixed terrestrial/ aquatic diet. Our results provide support for complex interactions among hunter-gatherers and farmers in the Danube basin, demonstrating that in some regions, demic and cultural diffusion were not mutually exclusive, but merely the ends of a continuum for the process of Neolithization. Y1 - 2017 U6 - https://doi.org/10.1016/j.cub.2017.05.023 SN - 0960-9822 SN - 1879-0445 VL - 27 SP - 1801 EP - + PB - Cell Press CY - Cambridge ER -