TY - JOUR A1 - Ruszkiewicz, Joanna A1 - Papatheodorou, Ylea A1 - Jäck, Nathalie A1 - Melzig, Jasmin A1 - Eble, Franziska A1 - Pirker, Annika A1 - Thomann, Marius A1 - Haberer, Andreas A1 - Rothmiller, Simone A1 - Bürkle, Alexander A1 - Mangerich, Aswin T1 - NAD+ Acts as a protective factor in cellular stress response to DNA alkylating agents JF - Cells : open access journal N2 - Sulfur mustard (SM) and its derivatives are potent genotoxic agents, which have been shown to trigger the activation of poly (ADP-ribose) polymerases (PARPs) and the depletion of their substrate, nicotinamide adenine dinucleotide (NAD+). NAD+ is an essential molecule involved in numerous cellular pathways, including genome integrity and DNA repair, and thus, NAD+ supplementation might be beneficial for mitigating mustard-induced (geno)toxicity. In this study, the role of NAD+ depletion and elevation in the genotoxic stress response to SM derivatives, i.e., the monofunctional agent 2-chloroethyl-ethyl sulfide (CEES) and the crosslinking agent mechlorethamine (HN2), was investigated with the use of NAD+ booster nicotinamide riboside (NR) and NAD+ synthesis inhibitor FK866. The effects were analyzed in immortalized human keratinocytes (HaCaT) or monocyte-like cell line THP-1. In HaCaT cells, NR supplementation, increased NAD+ levels, and elevated PAR response, however, did not affect ATP levels or DNA damage repair, nor did it attenuate long- and short-term cytotoxicities. On the other hand, the depletion of cellular NAD+ via FK866 sensitized HaCaT cells to genotoxic stress, particularly CEES exposure, whereas NR supplementation, by increasing cellular NAD+ levels, rescued the sensitizing FK866 effect. Intriguingly, in THP-1 cells, the NR-induced elevation of cellular NAD+ levels did attenuate toxicity of the mustard compounds, especially upon CEES exposure. Together, our results reveal that NAD+ is an important molecule in the pathomechanism of SM derivatives, exhibiting compound-specificity. Moreover, the cell line-dependent protective effects of NR are indicative of system-specificity of the application of this NAD+ booster. KW - nicotinamide adenine dinucleotide KW - NAD booster; KW - mustard agents KW - nicotinamide riboside KW - DNA damage KW - sulfur mustard KW - poly(ADP-ribosylation) KW - PARP Y1 - 2023 U6 - https://doi.org/10.3390/cells12192396 SN - 2073-4409 VL - 12 IS - 19 PB - MDPI CY - Basel ER - TY - JOUR A1 - Sagu Tchewonpi, Sorel A1 - Rawel, Harshadrai M. A1 - Rohn, Sascha T1 - Targeted bottom-up mass spectrometry approach for the relative quantification of post-translational modification of bovine κ-casein during milk fermentation JF - Molecules N2 - kappa-casein (kappa-CN) is one of the key components in bovine milk, playing a unique role in the structuration of casein micelles. It contains in its chemical structure up to sixteen amino acid residues (mainly serine and threonine) susceptible to modifications, including glycosylation and phosphorylation, which may further be formed during milk processing. In this study, changes in post-translational modification (PTM) of kappa-CN during bovine milk fermentation were investigated. One-to-five-day fermented milk samples were produced. A traditional bottom-up proteomics approach was used to establish a multiple-reaction monitoring (MRM) method for relative quantification of kappa-CN PTM. Endoproteinase Glu-C was found to efficiently digest the kappa-CN molecule. The developed LC-MS method was validated by performing assessments of linearity, precision, repeatability, reproducibility, limit of detection (LOD), and limit of quantification (LOQ). Among the yielded peptides, four of them containing serine and threonine residues were identified and the unmodified as well as the modified variants of each of them were relatively quantified. These peptides were (1) IPTINTIASGEPTSTTE ([140, 158]), (2) STVATLE ([162, 168]), (3) DSPE ([169, 172]), and (4) INTVQVTSTAV ([180, 190]). Distribution analysis between unmodified and modified peptides revealed that over 50% of kappa-CN was found in one of its modified forms in milk. The fermentation process further significantly altered the composition between unmodified/modified kappa-CN, with glycoslaytion being predominant compared to phosphorylation (p < 0.01). Further method development towards alpha and beta-CN fractions and their PTM behavior would be an asset to better understand the changes undergone by milk proteins and the micellar structure during fermentation. KW - bovine milk KW - fermentation KW - kappa-casein KW - post-translational modifications KW - glycosylation KW - phosphorylation KW - mass spectrometry Y1 - 2022 U6 - https://doi.org/10.3390/molecules27185834 SN - 1420-3049 VL - 27 IS - 18 PB - MDPI CY - Basel ER - TY - JOUR A1 - Horvat, Anja Kranjc A1 - Wiener, Jeff A1 - Schmeling, Sascha Marc A1 - Borowski, Andreas T1 - What does the curriculum say? Review of the particle physics content in 27 high-school physics curricula JF - Physics N2 - This international curricular review provides a structured overview of the particle physics content in 27 state, national, and international high-school physics curricula. The review was based on a coding manual that included 60 concepts that were identified as relevant for high-school particle physics education. Two types of curricula were reviewed, namely curricula with a dedicated particle physics chapter and curricula without a dedicated particle physics chapter. The results of the curricular review show that particle physics concepts are explicitly or implicitly present in all reviewed curricula. However, the number of particle physics concepts that are featured in a curriculum varies greatly across the reviewed curricula. We identified core particle physics concepts that can be found in most curricula. Here, elementary particles, fundamental interactions, and charges were identified as explicit particle physics concepts that are featured in more than half of the reviewed curricula either as content or context. Indeed, theoretical particle physics concepts are more prominent in high-school physics curricula than experimental particle physics concepts. Overall, this international curricular review provides the basis for future curricular development with respect to particle physics and suggests an increased inclusion of experimental particle physics concepts in high-school physics curricula. KW - curricular review KW - particle physics KW - high-school education Y1 - 2022 U6 - https://doi.org/10.3390/physics4040082 SN - 2624-8174 VL - 4 IS - 4 SP - 1278 EP - 1298 PB - MDPI CY - Basel ER - TY - CHAP A1 - Grum, Marcus ED - Shishkov, Boris T1 - Managing multi-site artificial neural networks’ activation rates and activation cycles T2 - Business modeling and software design : 14th International Symposium, BMSD 2024, Luxembourg City, Luxembourg, July 1–3, 2024, proceedings N2 - Traditionally, business models and software designs used to model the usage of artificial intelligence (AI) at a very specific point in the process or rather fix implemented application. Since applications can be based on AI, such as networked artificial neural networks (ANN) on top of which applications are installed, these on-top applications can be instructed directly from their underlying ANN compartments [1]. However, with the integration of several AI-based systems, their coordination is a highly relevant target factor for the operation and improvement of networked processes, such as they can be found in cross-organizational production contexts spanning multiple distributed locations. This work aims to extend prior research on managing artificial knowledge transfers among interlinked AIs as coordination instrument by examining effects of different activation types (respective activation rates and cycles) on by ANN-instructed production machines. In a design-science-oriented way, this paper conceptualizes rhythmic state descriptions for dynamic systems and associated 14 experiment designs. Two experiments have been realized, analyzed and evaluated thereafter in regard with their activities and processes induced. Findings show that the simulator [2] used and experiments designed and realized, here, (I) enable research on ANN activation types, (II) illustrate ANN-based production networks disrupted by activation types and clarify the need for harmonizing them. Further, (III) management interventions are derived for harmonizing interlinked ANNs. This study establishes the importance of site-specific coordination mechanisms and novel forms of management interventions as drivers of efficient artificial knowledge transfer. Y1 - 2024 SN - 978-3-031-64072-8 SN - 978-3-031-64073-5 U6 - https://doi.org/10.1007/978-3-031-64073-5_17 SP - 258 EP - 269 PB - Springer CY - Cham ER - TY - JOUR A1 - Shlapunov, Alexander A. A1 - Tarchanov, Nikolaj Nikolaevič T1 - Inverse image of precompact sets and regular solutions to the Navier-Stokes equations JF - Vestnik Udmurtskogo Universiteta. Matematika, mechanika, kompʹjuternye nauki N2 - We consider the initial value problem for the Navier-Stokes equations over R-3 x [0, T] with time T > 0 in the spatially periodic setting. We prove that it induces open injective mappings A(s): B-1(s) -> B-2(s-1) where B-1(s), B-2(s-1) are elements from scales of specially constructed function spaces of Bochner-Sobolev typeparametrized with the smoothness index s is an element of N. Finally, we prove that a map Asis surjective if and only if the inverse image A(s)(- 1) (K) of any pre compact set K from the range of the map Asis bounded in the Bochner space L-s([0, T], L-r(T-3))with the Ladyzhenskaya-Prodi-Serrin numbers s, r. KW - Navier-Stokes equations KW - regular solutions Y1 - 2022 U6 - https://doi.org/10.35634/vm220208 SN - 1994-9197 SN - 2076-5959 VL - 32 IS - 2 SP - 278 EP - 297 PB - Udmurtskij gosudarstvennyj universitet CY - Iževsk ER - TY - CHAP A1 - Grum, Marcus ED - Shishkov, Boris T1 - Researching multi-site artificial neural networks’ activation rates and activation cycles T2 - Business modeling and software design : 14th International Symposium, BMSD 2024, Luxembourg City, Luxembourg, July 1–3, 2024, proceedings N2 - With the further development of more and more production machines into cyber-physical systems, and their greater integration with artificial intelligence (AI) techniques, the coordination of intelligent systems is a highly relevant target factor for the operation and improvement of networked processes, such as they can be found in cross-organizational production contexts spanning multiple distributed locations. This work aims to extend prior research on managing their artificial knowledge transfers as coordination instrument by examining effects of different activation types (respective activation rates and cycles) on by Artificial Neural Network (ANN)-instructed production machines. For this, it provides a new integration type of ANN-based cyber-physical production system as a tool to research artificial knowledge transfers: In a design-science-oriented way, a prototype of a simulation system is constructed as Open Source information system which will be used in on-building research to (I) enable research on ANN activation types in production networks, (II) illustrate ANN-based production networks disrupted by activation types and clarify the need for harmonizing them, and (III) demonstrate conceptual management interventions. This simulator shall establish the importance of site-specific coordination mechanisms and novel forms of management interventions as drivers of efficient artificial knowledge transfer. Y1 - 2024 SN - 978-3-031-64072-8 SN - 978-3-031-64073-5 U6 - https://doi.org/10.1007/978-3-031-64073-5_12 SP - 186 EP - 206 PB - Springer CY - Cham ER - TY - JOUR A1 - Lück, Erika A1 - Guillemoteau, Julien A1 - Tronicke, Jens A1 - Rummel, Udo A1 - Hierold, Wilfried T1 - From point to field scale-indirect monitoring of soil moisture variations at the DWD test site in Falkenberg JF - Geoderma : an international journal of soil science N2 - Information regarding the spatial distribution of soil water content is key in many disciplines and applications including soil and atmospheric sciences, hydrology, and agricultural engineering. Thus, within the past decades various experimental methods and strategies have been developed to map spatial variations in soil moisture distribution and to monitor temporal changes. Our study examines the combination of electrical resistivity mapping and point observations of soil moisture to infer the spatial and the temporal variability of soil moisture. Over a period of around two years, we performed field measurements on six days to collect repeated electrical resistivity mapping data for a nine-hectare test site south-east of Berlin, Germany. Permanently installed TDR probes, temporary TDR measurements within permanently installed tubes, and gravimetric measurements using soil samples provided soil moisture data at various selected points. In addition, soil analysis and classification results are available for 132 regularly distributed positions up to depths of 1.2 m. We compare and link three-dimensional resistivity models obtained via data inversion to soil composition and soil moisture as provided by our point data. Both the soil samples and the resistivity models indicate a two-layer medium characterized by a sandy top layer with varying thickness and a loamy bottom soil. For all six field campaigns, we observe similar resistivity patterns reflecting the temporally stable influence of soil texture. While the overall patterns are stable, the range of resistivity values changes with soil moisture. Finally, to estimate spatial models of soil moisture, we link our soil moisture and resistivity data using empirical petrophysical models relying on a second order polynomial function. We observe a mean prediction error for soil moisture of +/-0.034 m3 & BULL; m? 3 using all observation points while we notice that point-specific models further reduce the error. Thus, we conclude that our experimental and data analysis strategies represent a reliable approach to establish site-specific models and to estimate three-dimensional moisture distribution including temporal variations. KW - earth boundary layer KW - electrical conductivity KW - electrical resistivity KW - near-surface geophysics KW - soil moisture at field scale Y1 - 2022 U6 - https://doi.org/10.1016/j.geoderma.2022.116134 SN - 0016-7061 SN - 1872-6259 VL - 427 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Stein, Lina A1 - Mukkavilli, Surya Karthik A1 - Wagener, Thorsten T1 - Lifelines for a drowning science - improving findability and synthesis of hydrologic publications JF - Hydrological processes N2 - Increasing publication numbers make it difficult to keep up with knowledge evolution in a science like hydrology. Here we give recommendations to authors and journals for writing future-proof articles that contribute to knowledge accumulation and synthesis. KW - findability KW - hydrology KW - knowledge synthesis KW - natural language processing KW - scholarly publishing KW - text mining Y1 - 2022 U6 - https://doi.org/10.1002/hyp.14742 SN - 0885-6087 SN - 1099-1085 VL - 36 IS - 11 PB - Wiley CY - New York, NY ER - TY - CHAP A1 - Liese, Andrea ED - Orchard, Phil ED - Wiener, Antje T1 - Norm research on conflictive norm relations how norms collide in times of complexity, contestation, and crisis T2 - Contesting the world Y1 - 2024 SN - 978-1-00-947917-2 SN - 978-1-009-47916-5 SN - 978-1-009-47914-1 SP - 64 EP - 84 PB - Cambridge University Press CY - Cambridge ER - TY - JOUR A1 - Pasechnik, Sergey V. A1 - Shmeleva, Dina A1 - Saidgaziev, Ayvr Sh. A1 - Kharlamov, Semen A1 - Vasilieva, Aleksandra A. A1 - Santer, Svetlana T1 - Shear flows induced by electro-osmotic pumps in optofluidic liquid crystal cell for modulation of visible light and THz irradiation JF - Liquid Crystals and their Application : Russian Journal N2 - The work is devoted to the use of electrokinetic phenomena in liquid crystals to create a new class of microfluidics devices - optofluidics, designed to control electromagnetic radiation, including the THz frequency range. To achieve the goal, an optical method is used to study changes in the orientational structure in LC layers caused by a shear flow generated by electroosmotic pumps. Simula-tion of LC behaviour in an experimental cell containing electroosmotic pumps and flat layers of a nematic liquid crystal is fulfilled. The experimental depend-ences of the intensity of polarized radiation passing through flat LC layers on the control voltage applied to the electroosmotic pump and the results of calcu-lations of the hydrodynamic and mechano-optical characteristics of the experi-mental LC cell are presented. The propagation of THz irradiation across the multilayer structure of the optofluidic cell is considered taking into account the minimum number of re-reflections of waves from different layers and the ab-sorption of THz irradiation in a propylene and a liquid crystal. N2 - Работа посвящена использованию электрокинетических явлений в жидких кристаллах для создания нового класса микрофлюидных устройств – опто- флюидики, предназначенных для управления электромагнитным излуче- нием, в том числе терагерцового диапазона частот. Для достижения цели оптическим методом исследуются изменения ориентационной структуры в слоях ЖК, вызванные сдвиговым течением, генерируемым электроосмоти- ческими насосами. Проведено моделирование поведения ЖК в эксперимен- тальной ячейке, содержащей электроосмотические насосы и плоские слои нематического жидкого кристалла. Представлены экспериментальные за- висимости интенсивности поляризованного излучения, проходящего через плоские слои ЖК, от управляющего напряжения, подаваемого на электро- осмотический насос, и результаты расчетов гидродинамических и механо- оптических характеристик экспериментальной ЖК-ячейки. Рассмотрено распространение ТГц излучения по многослойной структуре оптофлюид- ной ячейки с учетом минимального числа переотражений волн от разных слоев и поглощения ТГц излучения в пропилене и жидком кристалле. KW - nematic liquid crystal KW - terahertz range KW - electrokinetic phenomena KW - osmotic flow KW - director configuration KW - optical irradiation intensity KW - E7 KW - polypropylene Y1 - 2022 U6 - https://doi.org/10.18083/LCAppl.2022.3.49 SN - 1991-3966 SN - 2499–9644 VL - 22 IS - 3 SP - 49 EP - 57 PB - Nanomaterials Research Institute CY - Ivanovo ER -