TY - JOUR A1 - Baral, Hans Otto A1 - Rönsch, Peter A1 - Richter, Udo A1 - Urban, Alexander A1 - Kruse, Julia A1 - Bemmann, Martin A1 - Kummer, Volker A1 - Javier Valencia, Francisco A1 - Huth, Wolfgang T1 - Schroeteria decaisneana, S. poeltii, and Ciboria ploettneriana (Sclerotiniaceae, Helotiales, Ascomycota), three parasites on Veronica seeds BT - first report of teleomorphs in Schroeteria JF - Mycological progress : international journal of the German Mycological Society N2 - Ciboria ploettneriana, Schroeteria decaisneana, and S. poeltii produce morphologically very similar apothecia emerging from fallen stromatized seeds of Veronica spp., the former two on V. hederifolia agg. in temperate central Europe and S. poeltii on V. cymbalaria in mediterranean southern Europe. They are described and illustrated in detail based on fresh collections or moist chamber cultures of infected seeds. A key is provided to differentiate the three species from their teleomorphs. For the first time, connections between two teleomorphs and two Schroeteria anamorphs are reported. Members of the anamorph-typified genus Schroeteria are known as host-specific plant parasites that infect seeds of different Veronica spp. In earlier times, they were classified in the Ustilaginales (Basidiomycota), but since more than 30 years, they are referred to as false smut fungi producing smut-like chlamydospores, based on light microscopic and ultrastructural studies which referred them to the Sclerotiniaceae (Helotiales). During the present study, rDNA sequences were obtained for the first time from chlamydospores of Schroeteria bornmuelleri (on V. rubrifolia), S. decaisneana (on V. hederifolia), S. delastrina (generic type, on V. arvensis), and S. poeltii (on V. cymbalaria) and from apothecia of C. ploettneriana, S. decaisneana, and S. poeltii. As a result, the anamorph-teleomorph connection could be established for S. decaisneana and S. poeltii by a 100% ITS similarity, whereas C. ploettneriana could not be connected to a smut-like anamorph. Ciboria ploettneriana in the here-redefined sense clustered in our combined phylogenetic analyses of ITS and LSU in relationship of Sclerotinia s.l., Botrytis, and Myriosclerotinia rather than Ciboria, but its placement was not supported. Its affiliation in Ciboria was retained until a better solution is found. Also Schroeteria poeltii clustered unresolved in this relationship but with a much higher molecular distance. The remaining three Schroeteria spp. formed a strongly supported monophyletic group, here referred to as "Schroeteria core clade", which clustered with medium to high support as a sister clade of Monilinia jezoensis, a member of the Monilinia alpina group of section Disjunctoriae. We observed ITS distances of 5-6.3% among members of the Schroeteria core clade, but 13.8-14.7% between this clade and S. poeltii, which appears to be correlated with the deviating chlamydospore morphology of S. poeltii. Despite its apparent paraphyly, Schroeteria is accepted here in a wide sense as a genus distinct from Monilinia, particularly because of its very special anamorphs. A comparable heterogeneity in rDNA analyses was observed in Monilinia and other genera of Sclerotiniaceae. Such apparent heterogeneity should be met with skepticism, however, because the inclusion of protein-coding genes in phylogenetic analyses resulted in a monophyletic genus Monilinia. More sclerotiniaceous taxa should be analysed for protein-coding genes in the future, including Schroeteria. Four syntype specimens of Ciboria ploettneriana in B were reexamined in the present study, revealing a mixture of the two species growing on V. hederifolia agg. Based on its larger ascospores in comparison with S. decaisneana, a lectotype is proposed for C. ploettneriana. KW - anamorph-teleomorph connection KW - plant parasite KW - false smut fungi KW - seed KW - infection KW - Veronica hederifolia KW - Veronica cymbalaria Y1 - 2022 U6 - https://doi.org/10.1007/s11557-021-01742-4 SN - 1617-416X SN - 1861-8952 VL - 21 IS - 1 SP - 359 EP - 407 PB - Springer CY - Berlin ; Heidelberg ER - TY - JOUR A1 - Kruse, Julia A1 - Kummer, Volker A1 - Shivas, Roger G. A1 - Thines, Marco T1 - The first smut fungus, Thecaphora anthemidis sp nov (Glomosporiaceae), described from Anthemis (Asteraceae) JF - MycoKeys N2 - There are 63 known species of Thecaphora (Glomosporiaceae, Ustilaginomycotina), a third of which occur on Asteraceae. These smut fungi produce yellowish-brown to reddish-brown masses of spore balls in specific, mostly regenerative, plant organs. A species of Thecaphora was collected in the flower heads of Anthemis chia (Anthemideae, Asteraceae) on Rhodes Island, Greece, in 2015 and 2017, which represents the first smut record of a smut fungus on a host plant species in this tribe. Based on its distinctive morphology, host species and genetic divergence, this species is described as Thecaphora anthemidis sp. nov. Molecular barcodes of the ITS region are provided for this and several other species of Thecaphora. A phylogenetic and morphological comparison to closely related species showed that Th. anthemidis differed from other species of Thecaphora. Thecaphora anthemidis produced loose spore balls in the flower heads and peduncles of Anthemis chia unlike other flower-infecting species. KW - Glomosporiaceae KW - host specificity KW - internal transcribed spacer KW - molecular phylogenetics KW - smut fungi Y1 - 2018 U6 - https://doi.org/10.3897/mycokeys.41.28454 SN - 1314-4057 SN - 1314-4049 IS - 41 SP - 39 EP - 50 PB - Pensoft Publ. CY - Sofia ER - TY - JOUR A1 - Bubner, Ben A1 - Buchheit, Ramona A1 - Friedrich, Frank A1 - Kummer, Volker A1 - Scholler, Markus T1 - Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov. JF - MycoKeys N2 - Species of rust fungi of the genus Milesina (Pucciiastraceae, Pucciniales) are distributed mainly in northern temperate regions. They host-alternate between needles of fir (Abies spp.) and fronds of ferns (species of Polypodiales). Milesina species are distinguished based on host taxonomy and urediniospore morphology. In this study, 12 species of Milesina from Europe were revised. Specimens were examined by light and scanning electron microscopy for urediniospore morphology with a focus on visualising germ pores (number, size and position) and echinulation. In addition, barcode loci (ITS, nad6, 28S) were used for species delimitation and for molecular phylogenetic analyses. Barcodes of 72 Milesina specimens were provided, including 11 of the 12 species. Whereas urediniospore morphology features were sufficient to distinguish all 12 Milesina species except for 2 (M. blechni and M. kriegeriana), ITS sequences separated only 4 of 11 species. Sequencing with 28S and nad6 did not improve species resolution. Phylogenetic analysis, however, revealed four phylogenetic groups within Milesina that also correlate with specific urediniospore characters (germ pore number and position and echinulation). These groups are proposed as new sections within Milesina (sections Milesina, Vogesiacae M. Scholler & Bubner, sect. nov., Scolopendriorum M. Scholler & Bubner, sect. nov. and Carpaticae M. Scholler & Bubner, sect. nov.). In addition, Milesina woodwardiana Buchheit & M. Scholler, sp. nov. on Woodwardia radicans, a member of the type section Milesina, is newly described. An identification key for European Milesina species, based on urediniospore features, is provided. KW - Abies alba KW - Polypodiales KW - GBOL KW - germ pores KW - host alternation KW - Uredinopsis KW - Europe Y1 - 2019 U6 - https://doi.org/10.3897/mycokeys.48.30350 SN - 1314-4057 SN - 1314-4049 IS - 48 SP - 1 EP - 40 PB - Pensoft Publishers CY - Sofia ER - TY - GEN A1 - Kruse, Julia A1 - Kummer, Volker A1 - Shivas, Roger G. A1 - Thines, Marco T1 - The first smut fungus, Thecaphora anthemidis sp. nov. (Glomosporiaceae), described from Anthemis (Asteraceae) T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - There are 63 known species of Thecaphora (Glomosporiaceae, Ustilaginomycotina), a third of which occur on Asteraceae. These smut fungi produce yellowish-brown to reddish-brown masses of spore balls in specific, mostly regenerative, plant organs. A species of Thecaphora was collected in the flower heads of Anthemis chia (Anthemideae, Asteraceae) on Rhodes Island, Greece, in 2015 and 2017, which represents the first smut record of a smut fungus on a host plant species in this tribe. Based on its distinctive morphology, host species and genetic divergence, this species is described as Thecaphora anthemidis sp. nov. Molecular barcodes of the ITS region are provided for this and several other species of Thecaphora. A phylogenetic and morphological comparison to closely related species showed that Th. anthemidis differed from other species of Thecaphora. Thecaphora anthemidis produced loose spore balls in the flower heads and peduncles of Anthemis chia unlike other flower-infecting species. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 995 KW - Glomosporiaceae KW - host specificity KW - internal transcribed spacer KW - molecular phylogenetics KW - smut fungi Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-446377 SN - 1866-8372 SP - 39 EP - 50 ER - TY - JOUR A1 - Rottstock, Tanja A1 - Kummer, Volker A1 - Fischer, Markus A1 - Joshi, Jasmin Radha T1 - Rapid transgenerational effects in Knautia arvensis in response to plant community diversity JF - The journal of ecology N2 - 1. Plant species persistence in natural communities requires coping with biotic and abiotic challenges. These challenges also depend on plant community composition and diversity. Over time, biodiversity effects have been shown to be strengthened via increasing species complementarity in mixtures. Little is known, however, whether differences in community diversity and composition induce rapid transgenerational phenotypic adaptive differentiation during community assembly. We expect altered plant-plant and other biotic interactions (mutualists or antagonists) in high vs. low diverse communities to affect immediate within-and between-species trait differentiations due to competition for light and nutrients. 2. Three years after the initiation of a large-scale, long-term biodiversity experiment in Jena, Germany, we tested for effects of varying experimental plant community diversity (1-60 plant species; one to four plant functional groups) and composition (with or without legumes and/or grasses) on phenotypic differentiation and variation of the tall herb Knautia arvensis. We measured reproduction at different diversity levels in the Jena Experiment (residents hereafter) and, in an additional common garden experiment without competition, recorded subsequent offspring performance (i.e. growth, reproductive success and susceptibility to powdery mildew) to test for differentiation in phenotypic expression and variability. 3. We observed phenotypic differences among diversity levels with reduced fecundity of K. arvensis residents in more diverse communities. In the next generation grown under common garden conditions, offspring from high-diversity plots showed reduced growth (i.e. height) and lower reproduction (i.e. fewer infructescences), but increased phenotypic trait variability (e.g. in leaf width and powdery mildew presence) and also tended to be less susceptible to powdery mildew infection. 4. Community composition also affected Knautia parents and offspring. In the presence of legumes, resident plants produced more seeds (increased fecundity); however, germination rate of those seeds was reduced at an early seedling stage (reduced fertility). 5. Synthesis. We conclude that rapid transgenerational effects of community diversity and composition on both mean and variation of phenotypic traits among offspring exist. In addition to heritable variation, environmentally induced epigenetic and/or maternal processes matter for early plant community assembly and may also determine future species coexistence and community stability. KW - biodiversity effects KW - environmental conditions KW - fungal pathogen susceptibility KW - grassland communities KW - phenotypic variability KW - plant development and life-history traits KW - plant species diversity KW - plasticity KW - selection KW - transgenerational effects Y1 - 2017 U6 - https://doi.org/10.1111/1365-2745.12689 SN - 0022-0477 SN - 1365-2745 VL - 105 SP - 714 EP - 725 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Goerg, Marlena A1 - Ploch, Sebastian A1 - Kruse, Julia A1 - Kummer, Volker A1 - Runge, Fabian A1 - Choi, Young-Joon A1 - Thines, Marco T1 - Revision of Plasmopara (Oomycota, Peronosporales) parasitic to Impatiens JF - Mycological progress : international journal of the German Mycological Society N2 - The oomycete Plasmopara obducens was first described on wild Impatiens noli-tangere in Germany in 1877. About 125 years later the first occurrence of P. obducens on cultivated I. walleriana in the United Kingdom was reported, and a worldwide epidemic followed. Although this pathogen is a major threat for ornamental busy lizzy, the identity of the pathogen remained unconfirmed and the high host specificity observed for the genus Plasmopara cast doubts regarding its determination as P. obducens. In this study, using multigene phylogenies and morphological investigation, it is revealed that P. obducens on I. noli-tangere is not the conspecific with the pathogen affecting I. walleriana and another ornamental balsam, I. balsamina. As a consequence, the new names P. destructor and P. velutina are introduced for the pathogens of I. walleriana and I. balsamina, respectively. KW - Busy lizzy KW - Downy mildew KW - Horticulture KW - Lady slipper balsam KW - New species KW - Peronosporaceae KW - Taxonomy KW - Touch-me-not Y1 - 2017 U6 - https://doi.org/10.1007/s11557-017-1316-y SN - 1617-416X SN - 1861-8952 VL - 16 SP - 791 EP - 799 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Ali, Tahir A1 - Runge, Fabian A1 - Dutbayev, Ayan A1 - Schmuker, Angelika A1 - Solovyeva, Irina A1 - Nigrelli, Lisa A1 - Buch, Ann-Katrin A1 - Xia, Xiaojuan A1 - Ploch, Sebastian A1 - Orren, Ouria A1 - Kummer, Volker A1 - Paule, Juraj A1 - Celik, Ali A1 - Vakhrusheva, Ljudmila A1 - Gabrielyan, Ivan A1 - Thines, Marco T1 - Microthlaspi erraticum (Jord.) T. Ali et Thines has a wide distribution, ranging from the Alps to the Tien Shan JF - Flora : morphology, distribution, functional ecology of plants N2 - Microthlaspi is a predominantly Eurasian genus which also occurs in the northernmost parts of Africa (Maghreb). The most widespread species of the genus is M. perfoliatum, which can be found from Sweden to Algeria and from Portugal to China. The other species are thought to have much more confined distribution ranges, often covering only a few hundred kilometres. This is also believed for the diploid M. erraticum, which was recently re-appraised as a taxon independent from the tetra- to hexaploid M. perfoliatum. Previously, M. erraticum was believed to be present only in Central Europe, from the East of France to Slovenia. In order to gain a deeper understanding of the ecology, evolution and migration history of Microthlaspi it was the focus of the current study to investigate, if M. erraticum is present in habitats outside Central Europe, but with microclimates similar to Central Europe. It is demonstrated that M. erraticum is much more widespread than previously thought, while other lineages apart from M. perfoliatum s.str. and M. erraticum seem to have restricted distribution ranges. The latter species was observed from the Alps and their foreland, the Balkans, the mountainous areas around the Black Sea, Southern Siberia, as well as the Altai and Tien Shan mountains. This demonstrates a widespread occurrence of this easily-overlooked species. (C) 2016 Elsevier GmbH. All rights reserved. KW - Biogeography KW - Coluteocarpeae KW - Noccaea KW - Phylogeny KW - Species complex KW - Thlaspi perfoliatum Y1 - 2016 U6 - https://doi.org/10.1016/j.flora.2016.09.008 SN - 0367-2530 SN - 1618-0585 VL - 225 SP - 76 EP - 81 PB - American Chemical Society CY - Jena ER - TY - JOUR A1 - Choi, Young-Joon A1 - Klosterman, Steven J. A1 - Kummer, Volker A1 - Voglmayr, Hermann A1 - Shin, Hyeon-Dong A1 - Thines, Marco T1 - Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach JF - Molecular phylogenetics and evolution N2 - Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The family Peronosporaceae (Straminipila; Oomycota) consists of obligate biotrophic pathogens that cause downy mildew disease on angiosperms, including a large number of cultivated plants. In the largest downy mildew genus Peronospora, a phylogenetically complex clade includes the economically important downy mildew pathogens of spinach and beet, as well as the type species of the genus Peronospora. To resolve this complex clade at the species level and to infer evolutionary relationships among them, we used multi-locus phylogenetic analysis and species tree estimation. Both approaches discriminated all nine currently accepted species and revealed four previously unrecognized lineages, which are specific to a host genus or species. This is in line with a narrow species concept, i.e. that a downy mildew species is associated with only a particular host plant genus or species. Instead of applying the dubious name Peronospora farinosa, which has been proposed for formal rejection, our results provide strong evidence that Peronospora schachtii is an independent species from lineages on Atriplex and apparently occurs exclusively on Beta vulgaris. The members of the clade investigated, the Peronospora rumicis clade, associate with three different host plant families, Amaranthaceae, Caryophyllaceae, and Polygonaceae, suggesting that they may have speciated following at least two recent inter-family host shifts, rather than contemporary cospeciation with the host plants. (C) 2015 Elsevier Inc. All rights reserved. KW - Cospeciation KW - Host shift KW - Multi-locus phylogeny KW - Oomycetes KW - Peronospora farinosa KW - Species tree Y1 - 2015 U6 - https://doi.org/10.1016/j.ympev.2015.03.003 SN - 1055-7903 SN - 1095-9513 VL - 86 SP - 24 EP - 34 PB - Elsevier CY - San Diego ER - TY - JOUR A1 - Rottstock, Tanja A1 - Joshi, Jasmin Radha A1 - Kummer, Volker A1 - Fischer, Markus T1 - Higher plant diversity promotes higher diversity of fungal pathogens, while it decreases pathogen infection per plant JF - Ecology : a publication of the Ecological Society of America N2 - Fungal plant pathogens are common in natural communities where they affect plant physiology, plant survival, and biomass production. Conversely, pathogen transmission and infection may be regulated by plant community characteristics such as plant species diversity and functional composition that favor pathogen diversity through increases in host diversity while simultaneously reducing pathogen infection via increased variability in host density and spatial heterogeneity. Therefore, a comprehensive understanding of multi-host multi-pathogen interactions is of high significance in the context of biodiversity-ecosystem functioning. We investigated the relationship between plant diversity and aboveground obligate parasitic fungal pathogen ("pathogens" hereafter) diversity and infection in grasslands of a long-term, large-scale, biodiversity experiment with varying plant species (1-60 species) and plant functional group diversity (1-4 groups). To estimate pathogen infection of the plant communities, we visually assessed pathogen-group presence (i.e., rusts, powdery mildews, downy mildews, smuts, and leaf-spot diseases) and overall infection levels (combining incidence and severity of each pathogen group) in 82 experimental plots on all aboveground organs of all plant species per plot during four surveys in 2006. Pathogen diversity, assessed as the cumulative number of pathogen groups on all plant species per plot, increased log-linearly with plant species diversity. However, pathogen incidence and severity, and hence overall infection, decreased with increasing plant species diversity. In addition, co-infection of plant individuals by two or more pathogen groups was less likely with increasing plant community diversity. We conclude that plant community diversity promotes pathogen-community diversity while at the same time reducing pathogen infection levels of plant individuals. KW - biodiversity KW - ecosystem processes KW - ecosystem services KW - grasslands KW - multi-host-multi-pathogen interactions KW - obligate parasitic fungal pathogens KW - pathogen diversity KW - pathogen proneness KW - pathogen transmission KW - plant functional types Y1 - 2014 SN - 0012-9658 SN - 1939-9170 VL - 95 IS - 7 SP - 1907 EP - 1917 PB - Wiley CY - Washington ER - TY - JOUR A1 - Thines, Marco A1 - Kummer, Volker T1 - Diversity and species boundaries in floricolous downy mildews JF - Mycological progress : international journal of the German Mycological Society N2 - Floricolous downy mildews are a monophyletic group of members of the genus Peronospora (Oomycota, Peronosporales). These downy mildews can be found on a variety of families of the Asteridae, including Asteraceae, Campanulaceae, Dipsacaceae, Lamiaceae, and Orobanchaceae. With the exception of Peronospora radii, which can also cause economically relevant losses, sporulation usually takes place only on floral parts of their hosts. However, only very few specimens of these mostly inconspicuous downy mildews have so far been included in molecular phylogenies. Focusing on Lamiaceae, we have investigated multiple specimens of floricolous downy mildews for elucidating species boundaries and host specificity in this group. Based on both mitochondrial and nuclear loci, it became apparent that phylogenetic lineages in the Lamiaceae seem to be host genus specific and significant sequence diversity could be found between lineages. Based on distinctiveness in both phylogenetic reconstructions and morphology, the downy mildew on flowers of Stachys palustris is introduced as a new species, Peronospora jagei sp. nov., which can be morphologically distinguished from Peronospora stigmaticola by broader and shorter conidiospores. The diversity of the floricolous down mildews might be higher than previously assumed, although specimens from a much broader set of samples will be needed to confirm this view. KW - cox2 KW - Internal transcribed spacer KW - Peronosporaceae KW - Phylogeny Y1 - 2013 U6 - https://doi.org/10.1007/s11557-012-0837-7 SN - 1617-416X VL - 12 IS - 2 SP - 321 EP - 329 PB - Springer CY - Heidelberg ER -