TY  - JOUR
A1  - Nicolas, Philippe
A1  - Lecourieux, David
A1  - Kappel, Christian
A1  - Cluzet, Stephanie
A1  - Cramer, Grant
A1  - Delrot, Serge
A1  - Lecourieux, Fatma
T1  - The basic leucine zipper transcription factor abscisic acid responseelement-binding factor 2 is an important transcriptional regulator ofabscisic acid-dependent grape berry ripening processes
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - In grape (Vitis vinifera), abscisic acid (ABA) accumulates during fruit ripening and is thought to play a pivotal role in this process, but the molecular basis of this control is poorly understood. This work characterizes ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), a grape basic leucine zipper transcription factor belonging to a phylogenetic subgroup previously shown to be involved in ABA and abiotic stress signaling in other plant species. VvABF2 transcripts mainly accumulated in the berry, from the onset of ripening to the harvesting stage, and were up-regulated by ABA. Microarray analysis of transgenic grape cells overexpressing VvABF2 showed that this transcription factor up-regulates and/or modifies existing networks related to ABA responses. In addition, grape cells overexpressing VvABF2 exhibited enhanced responses to ABA treatment compared with control cells. Among the VvABF2-mediated responses highlighted in this study, the synthesis of phenolic compounds and cell wall softening were the most strongly affected. VvABF2 overexpression strongly increased the accumulation of stilbenes that play a role in plant defense and human health (resveratrol and piceid). In addition, the firmness of fruits from tomato (Solanum lycopersicum) plants overexpressing VvABF2 was strongly reduced. These data indicate that VvABF2 is an important transcriptional regulator of ABA-dependent grape berry ripening.
Y1  - 2014
U6  - https://doi.org/10.1104/pp.113.231977
SN  - 0032-0889
SN  - 1532-2548
VL  - 164
IS  - 1
SP  - 365
EP  - 383
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Lecourieux, Fatma
A1  - Kappel, Christian
A1  - Lecourieux, David
A1  - Serrano, Alejandra
A1  - Torres, Elizabeth
A1  - Arce-Johnson, Patricio
A1  - Delrot, Serge
T1  - An update on sugar transport and signalling in grapevine
JF  - Journal of experimental botany
N2  - In addition to their role as a source of reduced carbon, sugars may directly or indirectly control a wide range of activities in plant cells, through transcriptional and post-translational regulation. This control has been studied in detail using Arabidopsis thaliana, where genetic analysis offers many possibilities. Much less is known about perennial woody species. For several years, various aspects of sugar sensing and signalling have been investigated in the grape (Vitis vinifera L.) berry, an organ that accumulates high concentrations of hexoses in the vacuoles of flesh cells. Here we review various aspects of this topic: the molecular basis of sugar transport and its regulation by sugars in grapevine; the functional analysis of several sugar-induced genes; the effects of some biotic and abiotic stresses on the sugar content of the berry; and finally the effects of exogenous sugar supply on the ripening process in field conditions. A picture of complex feedback and multiprocess regulation emerges from these data.
KW  - Fruit biology
KW  - grapevine
KW  - signalling
KW  - stress
KW  - sugar
KW  - transport
Y1  - 2014
U6  - https://doi.org/10.1093/jxb/ert394
SN  - 0022-0957
SN  - 1460-2431
VL  - 65
IS  - 3
SP  - 821
EP  - 832
PB  - Oxford Univ. Press
CY  - Oxford
ER  -