TY  - JOUR
A1  - Sustr, David
A1  - Hlaváček, Antonín
A1  - Duschl, Claus
A1  - Volodkin, Dmitry
T1  - Multi-fractional analysis of molecular diffusion in polymer multilayers by FRAP
BT  - a new simulation-based approach
JF  - The journal of physical chemistry :   B, Condensed matter, materials, surfaces, interfaces & biophysical
N2  - Comprehensive analysis of the multifractional molecular diffusion provides a deeper understanding of the diffusion phenomenon in the fields of material science, molecular and cell biology, advanced biomaterials, etc. Fluorescence recovery after photobleaching (FRAP) is commonly employed to probe the molecular diffusion. Despite FRAP being a very popular method, it is not easy to assess multifractional molecular diffusion due to limited possibilities of approaches for analysis. Here we present a novel simulation-optimization-based approach (S-approach) that significantly broadens possibilities of the analysis. In the S-approach, possible fluorescence recovery scenarios are primarily simulated and afterward compared with a real measurement while optimizing parameters of a model until a sufficient match is achieved. This makes it possible to reveal multifractional molecular diffusion. Fluorescent latex particles of different size and fluorescein isothiocyanate in an aqueous medium were utilized as test systems. Finally, the S-approach has been used to evaluate diffusion of cytochrome c loaded into multilayers made of hyaluronan and polylysine. Software for evaluation of multifractional molecular diffusion by S-approach has been developed aiming to offer maximal versatility and user-friendly way for analysis.
Y1  - 2017
U6  - https://doi.org/10.1021/acs.jpcb.7b11051
SN  - 1520-6106
VL  - 122
IS  - 3
SP  - 1323
EP  - 1333
PB  - American Chemical Society
CY  - Washington
ER  -