TY  - JOUR
A1  - Finke, Hannah
A1  - Wandt, Viktoria Klara Veronika
A1  - Ebert, Franziska
A1  - Guttenberger, Nikolaus
A1  - Glabonjat, Ronald A.
A1  - Stiboller, Michael
A1  - Francesconi, Kevin A.
A1  - Raber, Georg
A1  - Schwerdtle, Tanja
T1  - Toxicological assessment of arsenic-containing phosphatidylcholines in HepG2 cells
N2  - Arsenolipids include a wide range of organic arsenic species that occur naturally in seafood and thereby contribute to human arsenic exposure. Recently arsenic-containing phosphatidylcholines (AsPCs) were identified in caviar, fish, and algae. In this first toxicological assessment of AsPCs, we investigated the stability of both the oxo- and thioxo-form of an AsPC under experimental conditions, and analyzed cell viability, indicators of genotoxicity and biotransformation in human liver cancer cells (HepG2). Precise toxicity data could not be obtained owing to the low solubility in the cell culture medium of the thioxo-form, and the ease of hydrolysis of the oxo-form, and to a lesser degree the thioxo-form. Hydrolysis resulted amongst others in the respective constituent arsenic-containing fatty acid (AsFA). Incubation of the cells with oxo-AsPC resulted in a toxicity similar to that determined for the hydrolysis product oxo-AsFA alone, and there were no indices for genotoxicity. Furthermore, the oxo-AsPC was readily taken up by the cells resulting in high cellular arsenic concentrations (50 μM incubation: 1112 ± 146 μM As cellular), whereas the thioxo-AsPC was substantially less bioavailable (50 μM incubation: 293 ± 115 μM As cellular). Speciation analysis revealed biotransformation of the AsPCs to a series of AsFAs in the culture medium, and, in the case of the oxo-AsPC, to as yet unidentified arsenic species in cell pellets. The results reveal the difficulty of toxicity studies of AsPCs in vitro, indicate that their toxicity might be largely governed by their arsenic fatty acid content and suggest a multifaceted human metabolism of food derived complex arsenolipids.
KW  - Biochemistry
KW  - Biological Sciences
KW  - Science and Mathematics
KW  - Books
KW  - Journals
Y1  - 2020
U6  - https://doi.org/10.1039/d0mt00073f
VL  - 12
IS  - 7
SP  - 1159
EP  - 1170
PB  - Oxford University
CY  - Cambridge
ER  - 
TY  - GEN
T1  - Annotationen
T1  - Annotations
N2  - Annotationen
KW  - Annotationen
KW  - Bücher
KW  - Internationale Politik
KW  - Annotations
KW  - Books
KW  - International Politics
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-33992
SN  - 0944-8101
ER  - 
TY  - GEN
T1  - Rezensionen
T1  - Reviews
N2  - Rezensionen: - Not der Armen, Egoismus der Reichen - Autoritärer Naher Osten - Schattenreich NVA?
KW  - Rezensionen
KW  - Bücher
KW  - Reviews
KW  - Books
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-34146
SN  - 0944-8101
ER  - 
TY  - GEN
T1  - Annotationen
T1  - Annotations
N2  - Annotationen zu den Büchern: - Die Agnellis. Die heimlichen Herrscher Italiens - Die Außen- und Friedenspolitik des Heiligen Stuhls - Moralkommunikation der Macht, politische Konstruktion sozialer Kohäsion im Wohlfahrtsstaat
KW  - Annotationen
KW  - Bücher
KW  - Internationale Politik
KW  - Annotations
KW  - Books
KW  - International Politics
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-34210
SN  - 0944-8101
ER  - 
TY  - GEN
T1  - Rezensionen
T1  - Reviews
N2  - Inhalt: - Mehr Strategie wagen! - Von UN- und US-Willigen - Die Zähmung der Exekutive
KW  - Rezensionen
KW  - Bücher
KW  - Reviews
KW  - Books
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-34266
SN  - 0944-8101
ER  -