TY  - JOUR
A1  - Mehterov, Nikolay
A1  - Balazadeh, Salma
A1  - Hille, Jacques
A1  - Toneva, Valentina
A1  - Müller-Röber, Bernd
A1  - Gechev, Tsanko S.
T1  - Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes
JF  - Plant physiology and biochemistry : an official journal of the Federation of European Societies of Plant Physiology
N2  - The Arabidopsis thaliana atr7 mutant is tolerant to oxidative stress induced by paraquat (PQ) or the catalase inhibitor aminotriazole (AT), while its original background loh2 and wild-type plants are sensitive. Both, AT and PQ which stimulate the intracellular formation of H2O2 or superoxide anions, respectively, trigger cell death in loh2 but do not lead to visible damage in atr7. To study gene expression during oxidative stress and ROS-induced programmed cell death, two platforms for multi-parallel quantitative real-time PCR (qRT-PCR) analysis of 217 antioxidant and 180 ROS marker genes were employed. The qRT-PCR analyses revealed AT- and PQ-induced expression of many ROS-responsive genes mainly in loh2, confirming that an oxidative burst plays a role in the activation of the cell death in this mutant. Some of the genes were specifically regulated by either AT or PQ serving as markers for particular types of ROS. Genes significantly induced by both AT and PQ in loh2 included transcription factors (ANAC042/JUB1, ANAC102, DREB19, HSFA2, RRTF1, ZAT10, ZAT12, ethylene-responsive factors), signaling compounds, ferritins, alternative oxidases, and antioxidant enzymes. Many of these genes were upregulated in atr7 compared to loh2 under non-stress conditions at the first time point, indicating that higher basal levels of ROS and higher antioxidant capacity in atr7 are responsible for the enhanced tolerance to oxidative stress and suggesting a possible tolerance against multiple stresses of this mutant.
KW  - Antioxidant genes
KW  - Reactive oxygen species
KW  - Stress tolerance
KW  - Transcription analysis
Y1  - 2012
U6  - https://doi.org/10.1016/j.plaphy.2012.05.024
SN  - 0981-9428
VL  - 59
SP  - 20
EP  - 29
PB  - Elsevier
CY  - Paris
ER  -