TY  - JOUR
A1  - Goetz, Klaus-Peter
A1  - Chmielewski, Frank M.
A1  - Goedeke, Kristin
A1  - Wolf, Kristine
A1  - Jander, Elisabeth
A1  - Sievers, Steven
A1  - Homann, Thomas
A1  - Huschek, Gerd
A1  - Rawel, Harshadrai Manilal
T1  - Assessment of amino acids during winter rest and ontogenetic development in sweet cherry buds (Prunus avium. L.)
JF  - Scientia horticulturae : an international journal sponsored by the International Society for Horticultural Science
N2  - This study examined changes in sweet cherry buds of ‘Summit’ cultivar in four seasons (2011/12–2014/15) with respect to the nitrogen (N) content and the profile of eight free amino acids (asparagine (Asn), aspartic acid (Asp), isoleucine (Ile), glutamine (Gln), glutamic acid (Glu), arginine (Arg), alanine (Ala), histidine (His)). The presented results are to our knowledge the first under natural conditions in fruit tree orchards with a high temporal resolution from the dormant stage until cluster development. The N content in the buds from October, during endo- and ecodormancy until the beginning of ontogenetic development was a relatively stable parameter in each of the four seasons. The N accumulation into the buds began after ‘swollen bud’ and significant differences were visible at ‘green tip’ with an N content of 3.24, 3.12, 3.08, 2.40 which increased markedly to the mean of ‘tight’ and ‘open cluster’ by 3.77%, 3.78%, 3.44% and 3.10% in 2012–2015, respectively. In the buds, levels of asparagine were higher (up to 44 mg g−1 DW−1) than aspartic acid (up to 2 mg g−1 DW−1) and aspartic acid higher than isoleucine (up to 0.83 mg g−1 DW−1). Levels of glutamine were higher (up to 25 mg g−1 DW−1) than glutamic acid (up to 20 mg g−1 DW−1). The course of the arginine content was higher in 2011/12 compared to 2012/13, 2013/14 and 2014/15 which showed only slight differences. The alanine content in the buds was denoted in the four seasons only by relatively minor changes. The histidine content was higher in 2011/12 and 2012/13 compared to 2013/14 and 2014/15 which showed a comparable pattern. For 6 amino acids (Asn, Asp, Ile, Glu, Arg, Ala), the highest content was observed in 2012/13, the warmest period between swollen bud and open cluster. However in 2014/15, the season with the lowest mean temperature of 8.8 °C, only the content of Gln was the lowest. It was not possible to explain any seasonal differences in the amino acid content by environmental factors (air temperature) on the basis of few seasons. From none of the measured free amino acids could a clear determination of the date of endodormancy release (t1) or the beginning of the ontogenetic development (t1*) be derived. Therefore, these amino acids are no suitable markers to improve phenological models for the beginning of cherry blossom.
KW  - Amino acids
KW  - Flower buds
KW  - Prunus avium L.
KW  - Dormancy
KW  - Ontogenetic development
Y1  - 2017
U6  - https://doi.org/10.1016/j.scienta.2017.05.001
SN  - 0304-4238
SN  - 1879-1018
VL  - 222
SP  - 102
EP  - 110
PB  - Elsevier
CY  - Amsterdam
ER  -