TY  - JOUR
A1  - Olas, Justyna Jadwiga
A1  - Wahl, Vanessa
T1  - Tissue-specific NIA1 and NIA2 expression in Arabidopsis thaliana
JF  - Plant Signaling & Behavior
N2  - Nitrogen (N) is an essential macronutrient for optimal plant growth and ultimately for crop productivity Nitrate serves as the main N source for most plants. Although it seems a well-established fact that nitrate concentration affects flowering, its molecular mode of action in flowering time regulation was poorly understood. We recently found how nitrate, present at the shoot apical meristem (SAM), controls flowering time In this short communication, we present data on the tissue-specific expression patterns of NITRATE REDUCTASE 1 (NIA1) and NIA2 in planta. We show that transcripts of both genes are present throughout the life cycle of Arabidopsis thaliana plants with NIA1 being predominantly active in leaves and NIA2 in meristematic tissues.
KW  - Arabidopsis
KW  - NIA1
KW  - NIA2
KW  - nitrate assimilation
KW  - plant development
KW  - RNA in situ hybridization
KW  - expression
KW  - cell
KW  - and tissue-specificity
Y1  - 2019
U6  - https://doi.org/10.1080/15592324.2019.1656035
SN  - 1559-2316
SN  - 1559-2324
VL  - 14
IS  - 11
PB  - Taylor & Francis Group
CY  - Philadelphia
ER  - 
TY  - JOUR
A1  - Muino, Jose M.
A1  - de Bruijn, Suzanne
A1  - Pajoro, Alice
A1  - Geuten, Koen
A1  - Vingron, Martin
A1  - Angenent, Gerco C.
A1  - Kaufmann, Kerstin
T1  - Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor
JF  - Molecular biology and evolution
N2  - Flower development is controlled by the action of key regulatory transcription factors of the MADS-domain family. The function of these factors appears to be highly conserved among species based on mutant phenotypes. However, the conservation of their downstream processes is much less well understood, mostly because the evolutionary turnover and variation of their DNA-binding sites (BSs) among plant species have not yet been experimentally determined. Here, we performed comparative ChIP (chromatin immunoprecipitation)-seq experiments of the MADS-domain transcription factor SEPALLATA3 (SEP3) in two closely related Arabidopsis species: Arabidopsis thaliana and A. lyrata which have very similar floral organ morphology. We found that BS conservation is associated with DNA sequence conservation, the presence of the CArG-box BS motif and on the relative position of the BS to its potential target gene. Differences in genome size and structure can explain that SEP3 BSs in A. lyrata can be located more distantly to their potential target genes than their counterparts in A. thaliana. In A. lyrata, we identified transposition as a mechanism to generate novel SEP3 binding locations in the genome. Comparative gene expression analysis shows that the loss/gain of BSs is associated with a change in gene expression. In summary, this study investigates the evolutionary dynamics of DNA BSs of a floral key-regulatory transcription factor and explores factors affecting this phenomenon.
KW  - MADS-domain transcription factor
KW  - cis-regulatory evolution
KW  - plant development
Y1  - 2016
U6  - https://doi.org/10.1093/molbev/msv210
SN  - 0737-4038
SN  - 1537-1719
VL  - 33
SP  - 185
EP  - 200
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Muiño, Jose M.
A1  - de Bruijn, Suzanne
A1  - Pajoro, Alice
A1  - Geuten, Koen
A1  - Vingron, Martin
A1  - Angenent, Gerco C.
A1  - Kaufmann, Kerstin
T1  - Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor
JF  - Molecular biology and evolution : MBE
N2  - lower development is controlled by the action of key regulatory transcription factors of the MADS-domain family. The function of these factors appears to be highly conserved among species based on mutant phenotypes. However, the conservation of their downstream processes is much less well understood, mostly because the evolutionary turnover and variation of their DNA-binding sites (BSs) among plant species have not yet been experimentally determined. Here, we performed comparative ChIP (chromatin immunoprecipitation)-seq experiments of the MADS-domain transcription factor SEPALLATA3 (SEP3) in two closely related Arabidopsis species: Arabidopsis thaliana and A. lyrata which have very similar floral organ morphology. We found that BS conservation is associated with DNA sequence conservation, the presence of the CArG-box BS motif and on the relative position of the BS to its potential target gene. Differences in genome size and structure can explain that SEP3 BSs in A. lyrata can be located more distantly to their potential target genes than their counterparts in A. thaliana. In A. lyrata, we identified transposition as a mechanism to generate novel SEP3 binding locations in the genome. Comparative gene expression analysis shows that the loss/gain of BSs is associated with a change in gene expression. In summary, this study investigates the evolutionary dynamics of DNA BSs of a floral key-regulatory transcription factor and explores factors affecting this phenomenon.
KW  - MADS-domain transcription factor
KW  - plant development
KW  - cis-regulatory evolution
Y1  - 2015
U6  - https://doi.org/10.1093/molbev/msv210
SN  - 1537-1719
SN  - 0737-4038
VL  - 33
IS  - 1
PB  - Oxford University Press
CY  - Oxford
ER  -