TY  - JOUR
A1  - Fruscalzo, Arrigo
A1  - Londero, Ambrogio P.
A1  - Driul, Lorenza
A1  - Henze, Andrea
A1  - Tonutti, Laura
A1  - Ceraudo, Maria
A1  - Zanotti, Giuseppe
A1  - Berni, Rodolfo
A1  - Schweigert, Florian J.
A1  - Raila, Jens
T1  - First trimester concentrations of the TTR-RBP4-retinol complex components as early markers of insulin-treated gestational diabetes mellitus
JF  - Clinical chemistry and laboratory medicine : journal of the Forum of the European Societies of Clinical Chemistry - the European Branch of the International Federation of Clinical Chemistry and Laboratory Medicine
N2  - Background: The objective of the study was to investigate the relationship between first trimester maternal serum levels of the TTR-RBP4-ROH complex components and the later insurgence of an altered glucose metabolism during pregnancy.
 Methods: Retrospective case control study including 96 patients between the 12th and 14th week of gestation, 32 that developed gestational diabetes mellitus (GDM), respectively, 21 non-insulin-treated (dGDM) and 11 insulin-treated (iGDM), 20 large for gestational age fetuses (LGA) without GDM and 44 patients with normal outcome as control. Serum concentrations of RBP4 and TTR were assessed by ELISA; serum concentration of ROH by reverse-phase high performance liquid chromatography (rpHPLC). The molecular heterogeneity of TTR and RBP4 was analyzed after immunoprecipitation by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
 Results: iGDM patients were characterized by reduced TTR, RBP4 and ROH compared to controls (respectively, iGDM vs. controls, mean +/- SD: TTR 3.96 +/- 0.89 mu mol/L vs. 4.68 +/- 1.21 mu mol/L, RBP4 1.13 +/- 0.25 mu mol/L vs. 1.33 +/- 0.38 mu mol/L and ROH 1.33 +/- 0.17 mu mol/L vs. 1.62 +/- 0.29 mu mol/L, p < 0.05). TTR containing Gly10 in place of Cys10 was lower in the iGDM group (p < 0.05) compared to controls. In the final logistic regression model ROH significantly predicted the diagnosis of iGDM (OR 0.93, 95% CI 0.87-0.98, p < 0.05).
 Conclusions: First trimester maternal serum ROH, RBP4 and TTR represent potential biomarkers associated with the development of iGDM.
KW  - first trimester
KW  - gestational diabetes mellitus (GDM)
KW  - insulin
KW  - large for gestational age fetus (LGA)
KW  - protein microheterogeneity
KW  - retinol (ROH)
KW  - serum retinol binding protein (RBP4)
KW  - transthyretin (TTR)
Y1  - 2015
U6  - https://doi.org/10.1515/cclm-2014-0929
SN  - 1434-6621
SN  - 1437-4331
VL  - 53
IS  - 10
SP  - 1643
EP  - 1651
PB  - De Gruyter
CY  - Berlin
ER  -