TY  - JOUR
A1  - Barlow, S. M.
A1  - Greig, J. B.
A1  - Bridges, J. W.
A1  - Carere, A.
A1  - Carpy, A. J.
A1  - Galli, Corrado L.
A1  - Kleiner, J.
A1  - Knudsen, I.
A1  - Koeter, H. B.
A1  - Levy, L. S.
A1  - Madsen, C.
A1  - Mayer, S.
A1  - Narbonne, J. F.
A1  - Pfannkuch, F.
A1  - Prodanchuk, M. G.
A1  - Smith, Mason R.
A1  - Steinberg, Pablo
T1  - Hazard identification by methods of animal-based toxicology
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Bartsch, Ingrid
A1  - Zschaler, Ingrid
A1  - Haseloff, Monika
A1  - Steinberg, Pablo
T1  - Establishment of a long-term culture system for rat colon epithelial cells
N2  - The aim of this study was to establish a long-term culture. system for rat colon epithelia isolaled by incubating a 4-cm-long rat colon segment cut longitudinally with all ethylenediaminetetraacetic acid [disodium salt]- containing buffer, taken up in conditioned medium from the normal rat kidney fibroblast cell line NRK (i.e., the supernatant Of pure NRK cultures), directly plated on mitomycin C-treated NRK cells and subcultured with conditioned medium from NRK cells. Cells started to migrate out of the crypts shortly after plating them on NRK feeder layers. Some of the crypts fell apart during the isolation procedure. whereas the vast majority of them did it within I to 2 Ill after plating. The cells proliferated extremely slowly but continuously over a period of 4 mo and were epithelial because they expressed cytokeratin 19 and were stained by crystal violet at pH 2.8. In conclusion, the experimental system described ill this study allows to maintain rat colon epithelial cells for up to 4 mo in culture and can be used to Study the effects of a variety of tumor-modulating factors on growth and gene expression of normal colon epithelial cells in vitro
Y1  - 2003
UR  - http://www.springerlink.com/content/120498/
U6  - https://doi.org/10.1290/0404035.1
SN  - 1071-2690
ER  - 
TY  - JOUR
A1  - Bluvshtein, Evgenia
A1  - Glass, George
A1  - Volohonsky, Gloria
A1  - Yaakubowitz, Margalit
A1  - Harness, Ella
A1  - Smorodinsky, Nechama
A1  - Seidel, Albrecht
A1  - Frank, Heinz
A1  - Stark, Avishay Abraham
A1  - Steinberg, Pablo
T1  - Inhibition of the hydrolytic and transpeptidatic activities of rat kidney gamma-glutamyltranspeptidase by specific monoclonal antibodies
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Dybing, E.
A1  - Doe, J.
A1  - Groten, J.
A1  - Kleiner, J.
A1  - O'Brien, J.
A1  - Renwick, A. G.
A1  - Schlatter, J.
A1  - Steinberg, Pablo
A1  - Tritschler, A.
A1  - Walker, R.
A1  - Younes, M.
T1  - Hazard characterisation of chemicals in food and diet : dose response, mechanisms and extrapolation issues
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Fuchs, J.
A1  - Teubner, Wera
A1  - Steinberg, Pablo
T1  - The resistance of intestinal epithelial cells towards the transforming activity of 2-hydroxyamino-1-methyl-6- phenylimidazo[4,5-B]pyridine is accompanied by glutathione S-transferase induction
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Hacker, Hans-Jörg
A1  - Steinberg, Pablo
A1  - Bannasch, Peter
T1  - Pyruvate kinase isoenzyme shift from L-type to M2-type is a late event in hepatocarcinogenesis induced in rats by a choline-deficient/DL-ethionine supplemented diet
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Hengstler, Jan Georg
A1  - Ringel, M.
A1  - Biefang, Katja
A1  - Hammel, S.
A1  - Milbert, U.
A1  - Gerl, M.
A1  - Klebach, M.
A1  - Diener, B.
A1  - Platt, Karl-Ludwig
A1  - Böttger, Thomas
A1  - Steinberg, Pablo
A1  - Oesch, Franz
T1  - Cultures with cryopreserved hepatocytes : applicability for studies of enzyme induction
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Hengstler, Jan Georg
A1  - Utesch, D.
A1  - Steinberg, Pablo
T1  - Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Hengstler, Jan Georg
A1  - VanDerBurg, Bart
A1  - Steinberg, Pablo
A1  - Oesch, Franz
T1  - Interspecies differences in cancer susceptibility and toxicity
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Herbst, Uta
A1  - Fuchs, Iris Judith
A1  - Teubner, Wera
A1  - Seidel, Albrecht
A1  - Frank, Heinz
A1  - Steinberg, Pablo
T1  - Malignant transformation of human colon epithelial cells by polycyclic aromatic hydrocarbons and heterocyclic aromatic amines
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Herbst, Uta
A1  - Fuchs, Iris Judith
A1  - Teubner, Wera
A1  - Steinberg, Pablo
T1  - Malignant transformation of human colon epithelial cells by benzo[c]phenanthrene dihydrodiolepoxides as well as 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine
N2  - Polycyclic aromatic hydrocarbons (PAHs) and heterocyclic aromatic amines (HCAs) ingested with food have repeatedly been suggested to be involved in the malignant transformation of colon epithelial cells. In order to test this hypothesis, HCEC cells (SV40 large T antigen-immortalized human colon epithelial cells) were incubated with a racemic mixture of benzo[c]phenanthrene dihydrodiol epoxides (B[c]PhDE), extremely potent carcinogenic PAH metabolites in vivo, or with 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP), the N-hydroxylated metabolite of the most abundant HCA in cooked meat. First, it was shown that HCEC cells express sulfotransferase 1A1, which is needed to metabolize N-OH-PhIP to the corresponding N-sulfonyloxy derivative, the direct precursor molecule of genotoxic nitrenium ions. Thereafter, exponentially growing HCEC cells were exposed five times to 0.1 mu g (0.37 nmol) B[c]PhDE/ml for 30 min or 0.72 mu g (3 mnol) N-OH-PhTP/ml for 24 h. Chemically treated HCEC cells showed an enhanced saturation density and grew faster than the corresponding solvent-treated cell cultures. After five treatment cycles, HCECB[c]PhDE as well as HCECN-OH-PhIP cells lost cell-cell contact inhibition and started piling up and forming foci in the culture flasks. Furthermore, HCECB[c]phDE and HCECN-OH-PhIP cells were injected i.m. into SCID mice. Within 6 weeks after injection, eight animals out of eight injected with HCECB[c]phDE or HCECN-OH-PhIP cells developed tumors at the site of injection, thus demonstrating the high tumorigenic potential of the HCECB[c]PhDE and HCECN-OH-PhIP cell cultures. Taken together, we show for the first time that the abovementioned active PAH metabolites as well as N-OH-PhIP are indeed able to malignantly transform human colon epithelial cells in vitro.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/0041008X
U6  - https://doi.org/10.1016/j.taap.2005.07.016
SN  - 0041-008X
ER  - 
TY  - JOUR
A1  - Komlosh, A.
A1  - Volohonsky, Gloria
A1  - Porat, Noga
A1  - Tuby, chen n. y. h.
A1  - Bluvshtein, Evgenia
A1  - Steinberg, Pablo
A1  - Oesch, Franz
A1  - Stark, Avishay Abraham
T1  - Gamma-glutamyl transpeptidase and glutathione biosynthesis in non-tumorigenic and tumorigenic rat liver oval cell lines
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Kühnel, Dana
A1  - Steinberg, Pablo
A1  - Scholtka, Bettina
T1  - A human-relevant dose of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) can induce precancerous lesions in rat intestine after 6 months of exposure
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Mazurek, Sybille
A1  - Eigenbrodt, Erich
A1  - Failing, Klaus
A1  - Steinberg, Pablo
T1  - Alterations in the glycolytic and glutaminolytic pathways after malignant transformation of rat liver oval cells
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Mueller, Carsten
A1  - Ullmann, Kristina
A1  - Wilkens, Andrea
A1  - Winterhalter, Peter
A1  - Toyokuni, Shinya
A1  - Steinberg, Pablo
T1  - Potent antioxidative activity of vineatrol (R) 30 grapevine-shoot extract
N2  - The health promoting effects of a grapevine-shoot extract named Vineatrol (R) 30, which contains resveratrol (Resv) as well as considerable amounts of Resv oligomers, have recently been investigated. In the present study, we analyzed the free radical scavenging capacity, the ability to inhibit lipid peroxidation, and the capacity to enhance the human glutathione peroxidase 1 (GPx) and the human superoxide dismutase 1 (SOD) gene promoter activities of Vineatrol (R) 30. Vineatrol (R) 30 was able to scavenge the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical cation and led to concentration-dependent inhibition of lipid peroxidation, Vineatrol (R) 30 not being superior to Resv alone in both cases. Vineatrol (R) 30 also enhanced the gene promoter activities of human GPx and SOD expressed in V79 cells, whereas this effect could not be demonstrated for Resv. In summary, the results presented in this study show that the Vineatrol (R) 30 grapevine-shoot extract is a free radical scavenger and potent antioxidant at non- eytotoxic concentrations.
Y1  - 2009
UR  - http://www.jstage.jst.go.jp/browse/bbb
U6  - https://doi.org/10.1271/Bbb.90213
SN  - 0916-8451
ER  - 
TY  - JOUR
A1  - Müller, Carsten
A1  - Ullmann, Kristina
A1  - Steinberg, Pablo
T1  - The grapevine-shoot extract Vineatrol30 Inhibits the chemically induced malignant transformation of BALB/c-3T3 Cells
JF  - Journal of medicinal food
N2  - Vineatrol (R) 30 (developed and produced jointly by Breko GmbH [Bremen, Germany] and Actichem [Montauban, France]) is a grapevine-shoot extract that contains resveratrol as well as considerable amounts of resveratrol oligomers. In the present study it is shown that Vineatrol30 at a noncytotoxic concentration of 2.3 mu g/mL significantly reduced the number of malignantly transformed foci induced by a sequential treatment of BALB/c-3T3 cells with 3-methylcholanthrene and 12-O-tetradecanoylphorbol 13-acetate in the so-called BALB/c-3T3 cell transformation assay. At a higher concentration Vineatrol30 drastically decreased the relative plating efficiency of the cells. Furthermore, the results suggest that the resveratrol oligomers present in Vineatrol30, independently from resveratrol itself, were indeed able to inhibit the formation of malignantly transformed BALB/c-3T3 foci.
KW  - BALB/c-3T3 cells
KW  - cell transformation assay
KW  - resveratrol
KW  - resveratrol oligomers
KW  - Vineatrol (R) 30
Y1  - 2011
U6  - https://doi.org/10.1089/jmf.2010.0022
SN  - 1096-620X
VL  - 14
IS  - 1-2
SP  - 34
EP  - 39
PB  - Liebert
CY  - New Rochelle
ER  - 
TY  - JOUR
A1  - Okano, J.
A1  - Shiota, G.
A1  - Matsumoto, K.
A1  - Yasui, S.
A1  - Kurimasa, A.
A1  - Hisatome, I.
A1  - Steinberg, Pablo
A1  - Murawaki, Y.
T1  - Hepatocyte growth factor exerts a proliferative effect on oval cells through the PI3K/AKT signaling pathway
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Schleger, C.
A1  - Becker, Rolf
A1  - Oesch, Franz
A1  - Steinberg, Pablo
T1  - The human p53 gene mutated at position 249 per se is not sufficient to immortalize human liver cells
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Schleger, C.
A1  - Heck, R.
A1  - Steinberg, Pablo
T1  - The role of wild-type and mutated N-ras in the malignant transformation of liver cells
Y1  - 2000
ER  - 
TY  - GEN
A1  - Scholtka, Bettina
A1  - Kühnel, Dana
A1  - Taugner, Felicitas
A1  - Steinberg, Pablo
T1  - Inflammation does not precede or accompany the induction of perneoplastic lesions in the colon of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-fed rats
N2  - Heterocyclic aromatic amines (HCAs) are formed in meat cooked at high temperatures for a long time or over an open flame. In this context 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), the most abundant HCA in cooked meat, has been suggested to be involved in colon and prostate carcinogenesis. In the latter case it has been reported that: (1) roughly 50% of Fischer F344 male rats treated with PhIP develop carcinomas in the ventral prostate lobe at 1 year of age; (2) inflammation precedes prostatic intraepithelial neoplasia in PhIP-fed rats; (3) inflammation specifically occurs in the ventral prostate lobe of PhIP-fed rats. To test whether PhIP by itself leads to inflammation in the colon and whether a human-relevant concentration of PhIP is able to induce preneoplastic lesions in the colon, male F344 rats were fed 0.1 or 100 ppm PhIP for up to 10 months and thereafter the colon tissue was analyzed histochemically. In none of the experimental groups signs of acute or chronic colonic inflammation were observed. 0.1 ppm PhIP leads to the development of hyperplastic and dysplastic lesions in the colon of single animals, but the incidence of these lesions does not reach a statistical significance. In contrast, in rats fed 100 ppm PhIP for 10 months hyperplastic and dysplastic colonic lesions were induced in a statistically significant number of animals. It is concluded that: (1) the induction of preneoplastic lesions in rat colon by PhIP is not preceded or accompanied by an inflammatory process; (2) a human-relevant concentration of PhIP alone is not sufficient to initiate colon carcinogenesis in rats.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 119 
KW  - Colorectal cancer
KW  - Heterocyclic aromatic amines
KW  - Inflammation
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44570
ER  -