TY  - GEN
A1  - Nakamura, Moritaka
A1  - Claes, Andrea R.
A1  - Grebe, Tobias
A1  - Hermkes, Rebecca
A1  - Viotti, Corrado
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Auxin and ROP GTPase signaling of polar nuclear migration in root epidermal hair cells
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Polar nuclear migration is crucial during the development of diverse eukaryotes. In plants, root hair growth requires polar nuclear migration into the outgrowing hair. However, knowledge about the dynamics and the regulatory mechanisms underlying nuclear movements in root epidermal cells remains limited. Here, we show that both auxin and Rho-of-Plant (ROP) signaling modulate polar nuclear position at the inner epidermal plasma membrane domain oriented to the cortical cells during cell elongation as well as subsequent polar nuclear movement to the outer domain into the emerging hair bulge in Arabidopsis (Arabidopsis thaliana). Auxin signaling via the nuclear AUXIN RESPONSE FACTOR7 (ARF7)/ARF19 and INDOLE ACETIC ACID7 pathway ensures correct nuclear placement toward the inner membrane domain. Moreover, precise inner nuclear placement relies on SPIKE1 Rho-GEF, SUPERCENTIPEDE1 Rho-GDI, and ACTIN7 (ACT7) function and to a lesser extent on VTI11 vacuolar SNARE activity. Strikingly, the directionality and/or velocity of outer polar nuclear migration into the hair outgrowth along actin strands also are ACT7 dependent, auxin sensitive, and regulated by ROP signaling. Thus, our findings provide a founding framework revealing auxin and ROP signaling of inner polar nuclear position with some contribution by vacuolar morphology and of actin-dependent outer polar nuclear migration in root epidermal hair cells.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 992 
KW  - Arabidopsis-thaliana
KW  - planar polarity
KW  - tip growth
KW  - morphogenesis
KW  - gene
KW  - proteins
KW  - dynamics
KW  - transformation
KW  - activation
KW  - initiation
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-441278
SN  - 1866-8372
IS  - 992
SP  - 378
EP  - 391
ER  - 
TY  - GEN
A1  - Merks, Anne Margarete
A1  - Swinarski, Marie
A1  - Meyer, Alexander Matthias
A1  - Müller, Nicola Victoria
A1  - Özcan, Ismail
A1  - Donat, Stefan
A1  - Burger, Alexa
A1  - Gilbert, Stephen
A1  - Mosimann, Christian
A1  - Abdelilah-Seyfried, Salim
A1  - Panáková, Daniela
T1  - Planar cell polarity signalling coordinates heart tube remodelling through tissue-scale polarisation of actomyosin activity
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Development of a multiple-chambered heart from the linear heart tube is inherently linked to cardiac looping. Although many molecular factors regulating the process of cardiac chamber ballooning have been identified, the cellular mechanisms underlying the chamber formation remain unclear. Here, we demonstrate that cardiac chambers remodel by cell neighbour exchange of cardiomyocytes guided by the planar cell polarity (PCP) pathway triggered by two non-canonical Wnt ligands, Wnt5b and Wnt11. We find that PCP signalling coordinates the localisation of actomyosin activity, and thus the efficiency of cell neighbour exchange. On a tissue-scale, PCP signalling planar-polarises tissue tension by restricting the actomyosin contractility to the apical membranes of outflow tract cells. The tissue-scale polarisation of actomyosin contractility is required for cardiac looping that occurs concurrently with chamber ballooning. Taken together, our data reveal that instructive PCP signals couple cardiac chamber expansion with cardiac looping through the organ-scale polarisation of actomyosin-based tissue tension.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 849 
KW  - convergent extension
KW  - branching morphogenesis
KW  - actin cytoskeleton
KW  - zebrafish heart
KW  - mouse heart
KW  - drosophila
KW  - cadherin
KW  - gene
KW  - differentiation
KW  - proliferation
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427026
SN  - 1866-8372
IS  - 849
ER  -