TY  - JOUR
A1  - Frescatada-Rosa, Marcia
A1  - Stanislas, Thomas
A1  - Backues, Steven K.
A1  - Reichardt, Ilka
A1  - Men, Shuzhen
A1  - Boutte, Yohann
A1  - Juergens, Gerd
A1  - Moritz, Thomas
A1  - Bednarek, Sebastian York
A1  - Grebe, Markus
T1  - High lipid order of Arabidopsis cell-plate membranes mediated by sterol and Dynamin-Related Protein 1A function
JF  - The plant journal
N2  - Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant cells is the membrane structure that separates daughter cells during somatic cytokinesis. Cell-plate formation in Arabidopsis relies on sterol- and DYNAMIN-RELATED PROTEIN1A (DRP1A)-dependent endocytosis. However, functional relationships between lipid membrane order or lipid packing and endocytic machinery components during eukaryotic cytokinesis have not been elucidated. Using ratiometric live imaging of lipid order-sensitive fluorescent probes, we show that the cell plate of Arabidopsis thaliana represents a dynamic, high lipid-order membrane domain. The cell-plate lipid order was found to be sensitive to pharmacological and genetic alterations of sterol composition. Sterols co-localize with DRP1A at the cell plate, and DRP1A accumulates in detergent-resistant membrane fractions. Modifications of sterol concentration or composition reduce cell-plate membrane order and affect DRP1A localization. Strikingly, DRP1A function itself is essential for high lipid order at the cell plate. Our findings provide evidence that the cell plate represents a high lipid-order domain, and pave the way to explore potential feedback between lipid order and function of dynamin-related proteins during cytokinesis.
KW  - membrane order
KW  - sterol
KW  - cytokinesis
KW  - DRP1A
KW  - Arabidopsis
Y1  - 2014
U6  - https://doi.org/10.1111/tpj.12674
SN  - 0960-7412
SN  - 1365-313X
VL  - 80
IS  - 5
SP  - 745
EP  - 757
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -