TY  - JOUR
A1  - Rosencrantz, Ruben R.
A1  - Vu Hoa Nguyen, 
A1  - Park, Hyunji
A1  - Schulte, Christine
A1  - Böker, Alexander
A1  - Schnakenberg, Uwe
A1  - Elling, Lothar
T1  - Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance
JF  - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis
N2  - A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.
KW  - Localized surface plasmon resonance
KW  - Glycopolymer brush
KW  - Microfluidics
KW  - Bacterial toxin
KW  - Glycosyltransferase
KW  - Biosensors
Y1  - 2016
U6  - https://doi.org/10.1007/s00216-016-9667-9
SN  - 1618-2642
SN  - 1618-2650
VL  - 408
SP  - 5633
EP  - 5640
PB  - Springer
CY  - Heidelberg
ER  -