TY  - JOUR
A1  - Samereier, Matthias
A1  - Baumann, Otto
A1  - Meyer, Irene
A1  - Gräf, Ralph
T1  - Analysis of dictyostelium TACC reveals differential interactions with CP224 and unusual dynamics of dictyostelium microtubules
JF  - Cellular and molecular life sciences
N2  - We have localized TACC to the microtubule-nucleating centrosomal corona and to microtubule plus ends. Using RNAi we proved that Dictyostelium TACC promotes microtubule growth during interphase and mitosis. For the first time we show in vivo that both TACC and XMAP215 family proteins can be differentially localized to microtubule plus ends during interphase and mitosis and that TACC is mainly required for recruitment of an XMAP215-family protein to interphase microtubule plus ends but not for recruitment to centrosomes and kinetochores. Moreover, we have now a marker to study dynamics and behavior of microtubule plus ends in living Dictyostelium cells. In a combination of live cell imaging of microtubule plus ends and fluorescence recovery after photobleaching (FRAP) experiments of GFP-alpha-tubulin cells we show that Dictyostelium microtubules are dynamic only in the cell periphery, while they remain stable at the centrosome, which also appears to harbor a dynamic pool of tubulin dimers.
KW  - Dictyostelium
KW  - TACC
KW  - DdCP224
KW  - XMAP215
KW  - Microtubules
KW  - Centrosome
Y1  - 2011
U6  - https://doi.org/10.1007/s00018-010-0453-0
SN  - 1420-682X
VL  - 68
IS  - 2
SP  - 275
EP  - 287
PB  - Springer
CY  - Basel
ER  -