TY  - JOUR
A1  - Fedyunin, Ivan
A1  - Lehnhardt, Lothar
A1  - Böhmer, Nadine
A1  - Kaufmann, Paul
A1  - Zhang, Gong
A1  - Ignatova, Zoya
T1  - tRNA concentration fine tunes protein solubility
JF  - FEBS letters : the journal for rapid publication of short reports in molecular biosciences
N2  - Clusters of codons pairing to low-abundance tRNAs synchronize the translation with co-translational folding of single domains in multidomain proteins. Although proven with some examples, the impact of the ribosomal speed on the folding and solubility on a global, cell-wide level remains elusive. Here we show that upregulation of three low-abundance tRNAs in Escherichia coil increased the aggregation propensity of several cellular proteins as a result of an accelerated elongation rate. Intriguingly, alterations in the concentration of the natural tRNA pool compromised the solubility of various chaperones consequently rendering the solubility of some chaperone-dependent proteins.
KW  - Protein translation
KW  - Protein misfolding
KW  - tRNA
KW  - E. coli
Y1  - 2012
U6  - https://doi.org/10.1016/j.febslet.2012.07.012
SN  - 0014-5793
VL  - 586
IS  - 19
SP  - 3336
EP  - 3340
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Lukoszek, Radoslaw
A1  - Müller-Röber, Bernd
A1  - Ignatova, Zoya
T1  - Interplay between polymerase II- and polymerase III-assisted expression of overlapping genes
JF  - FEBS letters : the journal for rapid publication of short reports in molecular biosciences
N2  - Up to 15% of the genes in different genomes overlap. This architecture, although beneficial for the genome size, represents an obstacle for simultaneous transcription of both genes. Here we analyze the interference between RNA-polymerase II (Pol II) and RNA-polymerase III (Pol III) when transcribing their target genes encoded on opposing strands within the same DNA fragment in Arabidopsis thaliana. The expression of a Pol II-dependent protein-coding gene negatively correlated with the transcription of a Pol III-dependent, tRNA-coding gene set. We suggest that the architecture of the overlapping genes introduces an additional layer of control of gene expression. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
KW  - Gene expression
KW  - Transcription
KW  - tRNA
KW  - Nested and overlapping genes
KW  - Arabidopsis thaliana
Y1  - 2013
U6  - https://doi.org/10.1016/j.febslet.2013.09.033
SN  - 0014-5793
SN  - 1873-3468
VL  - 587
IS  - 22
SP  - 3692
EP  - 3695
PB  - Elsevier
CY  - Amsterdam
ER  -