TY  - JOUR
A1  - Ma, Xuemin
A1  - Balazadeh, Salma
A1  - Mueller-Roeber, Bernd
T1  - Tomato fruit ripening factor NOR controls leaf senescence
JF  - Journal of experimental botany
N2  - NAC transcription factors (TFs) are important regulators of expressional reprogramming during plant development, stress responses, and leaf senescence. NAC TFs also play important roles in fruit ripening. In tomato (Solanum lycopersicum), one of the best characterized NACs involved in fruit ripening is NON-RIPENING (NOR), and the non-ripening (nor) mutation has been widely used to extend fruit shelf life in elite varieties. Here, we show that NOR additionally controls leaf senescence. Expression of NOR increases with leaf age, and developmental as well as dark-induced senescence are delayed in the nor mutant, while overexpression of NOR promotes leaf senescence. Genes associated with chlorophyll degradation as well as senescence-associated genes (SAGs) show reduced and elevated expression, respectively, in nor mutants and NOR overexpressors. Overexpression of NOR also stimulates leaf senescence in Arabidopsis thaliana. In tomato, NOR supports senescence by directly and positively regulating the expression of several senescence-associated genes including, besides others, SlSAG15 and SlSAG113, SlSGR1, and SlYLS4. Finally, we find that another senescence control NAC TF, namely SlNAP2, acts upstream of NOR to regulate its expression. Our data support a model whereby NAC TFs have often been recruited by higher plants for both the control of leaf senescence and fruit ripening.
KW  - Aging
KW  - leaf
KW  - NAC
KW  - non-ripening
KW  - NOR
KW  - senescence
KW  - tomato
KW  - transcription factor
Y1  - 2019
U6  - https://doi.org/10.1093/jxb/erz098
SN  - 0022-0957
SN  - 1460-2431
VL  - 70
IS  - 10
SP  - 2727
EP  - 2740
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Shahnejat-Bushehri, Sara
A1  - Allu, Annapurna Devi
A1  - Mehterov, Nikolay
A1  - Thirumalaikumar, Venkatesh P.
A1  - Alseekh, Saleh
A1  - Fernie, Alisdair R.
A1  - Mueller-Roeber, Bernd
A1  - Balazadeh, Salma
T1  - Arabidopsis NAC Transcription Factor JUNGBRUNNEN1 Exerts Conserved Control Over Gibberellin and Brassinosteroid Metabolism and Signaling Genes in Tomato
JF  - Frontiers in plant science
N2  - The Arabidopsis thaliana NAC transcription factor JUNGBRUNNEN1 (AtJUB1) regulates growth by directly repressing GA3ox1 and DWF4, two key genes involved in gibberellin (GA) and brassinosteroid (BR) biosynthesis, respectively, leading to GA and BR deficiency phenotypes. AtJUB1 also reduces the expression of PIF4, a bHLH transcription factor that positively controls cell elongation, while it stimulates the expression of DELLA genes, which are important repressors of growth. Here, we extend our previous findings by demonstrating that AtJUB1 induces similar GA and BR deficiency phenotypes and changes in gene expression when overexpressed in tomato (Solanum lycopersicum). Importantly, and in accordance with the growth phenotypes observed, AtJUB1 inhibits the expression of growth-supporting genes, namely the tomato orthologs of GA3ox1, DWF4 and PIF4, but activates the expression of DELLA orthologs, by directly binding to their promoters. Overexpression of AtJUB1 in tomato delays fruit ripening, which is accompanied by reduced expression of several ripeningrelated genes, and leads to an increase in the levels of various amino acids (mostly proline, beta-alanine, and phenylalanine), gamma-aminobutyric acid (GABA), and major organic acids including glutamic acid and aspartic acid. The fact that AtJUB1 exerts an inhibitory effect on the GA/BR biosynthesis and PIF4 genes but acts as a direct activator of DELLA genes in both, Arabidopsis and tomato, strongly supports the model that the molecular constituents of the JUNGBRUNNEN1 growth control module are considerably conserved across species.
KW  - Arabidopsis
KW  - tomato
KW  - fruit
KW  - growth
KW  - transcription factor
KW  - gibberellic acid
KW  - brassinosteroid
KW  - DELLA proteins
Y1  - 2017
U6  - https://doi.org/10.3389/fpls.2017.00214
SN  - 1664-462X
VL  - 8
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Lisso, Janina
A1  - Altmann, Thomas
A1  - Müssig, Carsten
T1  - Metabolic changes in fruits of the tomato d(x) mutant
JF  - Phytochemistry : an international journal of plant biochemistry
KW  - Solanum lycopersicum
KW  - Solanaceae
KW  - tomato
KW  - brassinosteroid
KW  - primary metabolism
KW  - fruit
Y1  - 2006
U6  - https://doi.org/10.1016/j.phytochem.2006.07.008
SN  - 0031-9422
VL  - 67
IS  - 20
SP  - 2232
EP  - 2238
PB  - Elsevier
CY  - Oxford
ER  -