TY  - JOUR
A1  - Sauer, Michael
A1  - Grebe, Markus
T1  - Plant cell biology
BT  - PIN polarity maintained
JF  - Current biology : CB
N2  - PIN-FORMED (PIN) polar protein localization directs transport of the growth and developmental regulator auxin in plants. Once established after cytokinesis, PIN polarity requires maintenance. Now, direct interactions between PIN, MAB4/MEL and PID proteins suggest self-reinforced maintenance of PIN polarity through limiting lateral diffusion.
Y1  - 2021
U6  - https://doi.org/10.1016/j.cub.2021.03.070
SN  - 0960-9822
SN  - 1879-0445
VL  - 31
IS  - 9
SP  - R449
EP  - R451
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Wang, Meng
A1  - Li, Panpan
A1  - Ma, Yao
A1  - Nie, Xiang
A1  - Grebe, Markus
A1  - Men, Shuzhen
T1  - Membrane sterol composition in Arabidopsis thaliana affects root elongation via auxin biosynthesis
JF  - International journal of molecular sciences
N2  - Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of cpi1-1 was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) was upregulated in cpi1-1. Functionally, TAA1 mutation reduced the auxin response in cpi1-1 and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter DR5:GUS (beta-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation.
KW  - Arabidopsis thaliana
KW  - auxin
KW  - auxin biosynthesis
KW  - cycloeucalenol
KW  - CPI1
KW  - sitosterol
KW  - sterol
Y1  - 2021
U6  - https://doi.org/10.3390/ijms22010437
SN  - 1422-0067
VL  - 22
IS  - 1
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Gendre, Delphine
A1  - Baral, Anirban
A1  - Dang, Xie
A1  - Esnay, Nicolas
A1  - Boutte, Yohann
A1  - Stanislas, Thomas
A1  - Vain, Thomas
A1  - Claverol, Stephane
A1  - Gustavsson, Anna
A1  - Lin, Deshu
A1  - Grebe, Markus
A1  - Bhalerao, Rishikesh P.
T1  - Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function
JF  - Development : Company of Biologists
N2  - Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.
KW  - ROP
KW  - YIP
KW  - Root hair
KW  - Secretion
KW  - Trans-Golgi network
Y1  - 2019
U6  - https://doi.org/10.1242/dev.168559
SN  - 0950-1991
SN  - 1477-9129
VL  - 146
IS  - 5
PB  - The Company of Biologists
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Chen, Hsiang-Wen
A1  - Persson, Staffan
A1  - Grebe, Markus
A1  - McFarlane, Heather E.
T1  - Cellulose synthesis during cell plate assembly
JF  - Physiologia plantarum
N2  - The plant cell wall surrounds and protects the cells. To divide, plant cells must synthesize a new cell wall to separate the two daughter cells. The cell plate is a transient polysaccharide-based compartment that grows between daughter cells and gives rise to the new cell wall. Cellulose constitutes a key component of the cell wall, and mutants with defects in cellulose synthesis commonly share phenotypes with cytokinesis-defective mutants. However, despite the importance of cellulose in the cell plate and the daughter cell wall, many open questions remain regarding the timing and regulation of cellulose synthesis during cell division. These questions represent a critical gap in our knowledge of cell plate assembly, cell division and growth. Here, we review what is known about cellulose synthesis at the cell plate and in the newly formed cross-wall and pose key questions about the molecular mechanisms that govern these processes. We further provide an outlook discussing outstanding questions and possible future directions for this field of research.
Y1  - 2018
U6  - https://doi.org/10.1111/ppl.12703
SN  - 0031-9317
SN  - 1399-3054
VL  - 164
IS  - 1
SP  - 17
EP  - 26
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Mao, Hailiang
A1  - Aryal, Bibek
A1  - Langenecker, Tobias
A1  - Hagmann, Jorg
A1  - Geisler, Markus
A1  - Grebe, Markus
T1  - Arabidopsis BTB/POZ protein-dependent PENETRATION3 trafficking and disease susceptibility
JF  - Nature plants
N2  - The outermost cell layer of plant roots (epidermis) constantly encounters environmental challenges. The epidermal outer plasma membrane domain harbours the PENETRATION3 (PEN3)/ABCG36/PDR8 ATP-binding cassette transporter that confers non-host resistance to several pathogens. Here, we show that the Arabidopsis ENDOPLASMIC RETICULUM-ARRESTED PEN3 (EAP3) BTB/POZ-domain protein specifically mediates PEN3 exit from the endoplasmic reticulum and confers resistance to a root-penetrating fungus, providing prime evidence for BTB/POZ-domain protein-dependent membrane trafficking underlying disease resistance.
Y1  - 2017
U6  - https://doi.org/10.1038/s41477-017-0039-z
SN  - 2055-026X
SN  - 2055-0278
VL  - 3
SP  - 854
EP  - 858
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Nakamura, Moritaka
A1  - Claes, Andrea R.
A1  - Grebe, Tobias
A1  - Hermkes, Rebecca
A1  - Viotti, Corrado
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Auxin and ROP GTPase Signaling of Polar Nuclear Migration in Root Epidermal Hair Cells
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - Polar nuclear migration is crucial during the development of diverse eukaryotes. In plants, root hair growth requires polar nuclear migration into the outgrowing hair. However, knowledge about the dynamics and the regulatory mechanisms underlying nuclear movements in root epidermal cells remains limited. Here, we show that both auxin and Rho-of-Plant (ROP) signaling modulate polar nuclear position at the inner epidermal plasma membrane domain oriented to the cortical cells during cell elongation as well as subsequent polar nuclear movement to the outer domain into the emerging hair bulge in Arabidopsis (Arabidopsis thaliana). Auxin signaling via the nuclear AUXIN RESPONSE FACTOR7 (ARF7)/ARF19 and INDOLE ACETIC ACID7 pathway ensures correct nuclear placement toward the inner membrane domain. Moreover, precise inner nuclear placement relies on SPIKE1 Rho-GEF, SUPERCENTIPEDE1 Rho-GDI, and ACTIN7 (ACT7) function and to a lesser extent on VTI11 vacuolar SNARE activity. Strikingly, the directionality and/or velocity of outer polar nuclear migration into the hair outgrowth along actin strands also are ACT7 dependent, auxin sensitive, and regulated by ROP signaling. Thus, our findings provide a founding framework revealing auxin and ROP signaling of inner polar nuclear position with some contribution by vacuolar morphology and of actin-dependent outer polar nuclear migration in root epidermal hair cells.
Y1  - 2017
U6  - https://doi.org/10.1104/pp.17.00713
SN  - 0032-0889
SN  - 1532-2548
VL  - 176
IS  - 1
SP  - 378
EP  - 391
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Poxson, David J.
A1  - Karady, Michal
A1  - Gabrielsson, Roger
A1  - Alkattan, Aziz Y.
A1  - Gustavsson, Anna
A1  - Doyle, Siamsa M.
A1  - Robert, Stephanie
A1  - Ljung, Karin
A1  - Grebe, Markus
A1  - Simon, Daniel T.
A1  - Berggren, Magnus
T1  - Regulating plant physiology with organic electronics
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - The organic electronic ion pump (OEIP) provides flow-free and accurate delivery of small signaling compounds at high spatio-temporal resolution. To date, the application of OEIPs has been limited to delivery of nonaromatic molecules to mammalian systems, particularly for neuroscience applications. However, many long-standing questions in plant biology remain unanswered due to a lack of technology that precisely delivers plant hormones, based on cyclic alkanes or aromatic structures, to regulate plant physiology. Here, we report the employment of OEIPs for the delivery of the plant hormone auxin to induce differential concentration gradients and modulate plant physiology. We fabricated OEIP devices based on a synthesized dendritic polyelectrolyte that enables electrophoretic transport of aromatic substances. Delivery of auxin to transgenic Arabidopsis thaliana seedlings in vivo was monitored in real time via dynamic fluorescent auxin-response reporters and induced physiological responses in roots. Our results provide a starting point for technologies enabling direct, rapid, and dynamic electronic interaction with the biochemical regulation systems of plants.
KW  - auxin
KW  - Arabidopsis thaliana
KW  - dendritic polymer
KW  - bioelectronics
KW  - polyelectrolyte
Y1  - 2017
U6  - https://doi.org/10.1073/pnas.1617758114
SN  - 0027-8424
VL  - 114
SP  - 4597
EP  - 4602
PB  - National Acad. of Sciences
CY  - Washington
ER  - 
TY  - JOUR
A1  - Mao, Hailiang
A1  - Nakamura, Moritaka
A1  - Viotti, Corrado
A1  - Grebe, Markus
T1  - A Framework for Lateral Membrane Trafficking and Polar Tethering of the PEN3 ATP-Binding Cassette Transporter
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of specifically outer membrane-localized proteins are known. Similarly, molecular mechanisms underlying the trafficking and the polar placement of outer membrane domain proteins require further exploration. Here, we demonstrate that ACTIN7 (ACT7) mediates trafficking of the PENETRATION3 (PEN3) outer membrane protein from the trans-Golgi network (TGN) to the plasma membrane in the root epidermis of Arabidopsis (Arabidopsis thaliana) and that actin function contributes to PEN3 endocytic recycling. In contrast to such generic ACT7-dependent trafficking from the TGN, the EXOCYST84b (EXO84b) tethering factor mediates PEN3 outer-membrane polarity. Moreover, precise EXO84b placement at the outer membrane domain itself requires ACT7 function. Hence, our results uncover spatially and mechanistically distinct requirements for ACT7 function during outer lateral membrane cargo trafficking and polarity establishment. They further identify an exocyst tethering complex mediator of outer lateral membrane cargo polarity.
Y1  - 2016
U6  - https://doi.org/10.1104/pp.16.01252
SN  - 0032-0889
SN  - 1532-2548
VL  - 172
SP  - 2245
EP  - 2260
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Krupinski, Pawel
A1  - Bozorg, Behruz
A1  - Larsson, Andre
A1  - Pietra, Stefano
A1  - Grebe, Markus
A1  - Jönsson, Henrik
T1  - A Model Analysis of Mechanisms for Radial Microtubular Patterns at Root Hair Initiation Sites
JF  - Frontiers in plant science
N2  - Plant cells have two main modes of growth generating anisotropic structures. Diffuse growth where whole cell walls extend in specific directions, guided by anisotropically positioned cellulose fibers, and tip growth, with inhomogeneous addition of new cell wall material at the tip of the structure. Cells are known to regulate these processes via molecular signals and the cytoskeleton. Mechanical stress has been proposed to provide an input to the positioning of the cellulose fibers via cortical microtubules in diffuse growth. In particular, a stress feedback model predicts a circumferential pattern of fibers surrounding apical tissues and growing primordia, guided by the anisotropic curvature in such tissues. In contrast, during the initiation of tip growing root hairs, a star-like radial pattern has recently been observed. Here, we use detailed finite element models to analyze how a change in mechanical properties at the root hair initiation site can lead to star-like stress patterns in order to understand whether a stress-based feedback model can also explain the microtubule patterns seen during root hair initiation. We show that two independent mechanisms, individually or combined, can be sufficient to generate radial patterns. In the first, new material is added locally at the position of the root hair. In the second, increased tension in the initiation area provides a mechanism. Finally, we describe how a molecular model of Rho-of-plant (ROP) GTPases activation driven by auxin can position a patch of activated ROP protein basally along a 2D root epidermal cell plasma membrane, paving the way for models where mechanical and molecular mechanisms cooperate in the initial placement and outgrowth of root hairs.
KW  - plant cell wall
KW  - finite element modeling
KW  - computational morphodynamics
KW  - root hair initiation
KW  - microtubules
KW  - cellulose fibers
KW  - composite material
Y1  - 2016
U6  - https://doi.org/10.3389/fpls.2016.01560
SN  - 1664-462X
VL  - 7
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Kiefer, Christian S.
A1  - Claes, Andrea R.
A1  - Nzayisenga, Jean-Claude
A1  - Pietra, Stefano
A1  - Stanislas, Thomas
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity
JF  - Development
N2  - The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.
KW  - AIP1
KW  - Actin
KW  - Arabidopsis
KW  - Patterning
KW  - Planar polarity
Y1  - 2015
UR  - http://dev.biologists.org/content/142/1/151.long
U6  - https://doi.org/doi: 10.1242/dev.111013
IS  - 142
SP  - 151
EP  - 161
ER  - 
TY  - JOUR
A1  - Pietra, Stefano
A1  - Lang, Patricia
A1  - Grebe, Markus
T1  - SABRE is required for stabilization of root hair patterning in Arabidopsis thaliana
JF  - Physiologia Plantarum
N2  - Patterned differentiation of distinct cell types is essential for the development of multicellular organisms. The root epidermis of Arabidopsis thaliana is composed of alternating files of root hair and non-hair cells and represents a model system for studying the control of cell-fate acquisition. Epidermal cell fate is regulated by a network of genes that translate positional information from the underlying cortical cell layer into a specific pattern of differentiated cells. While much is known about the genes of this network, new players continue to be discovered. Here we show that the SABRE (SAB) gene, known to mediate microtubule organization, anisotropic cell growth and planar polarity, has an effect on root epidermal hair cell patterning. Loss of SAB function results in ectopic root hair formation and destabilizes the expression of cell fate and differentiation markers in the root epidermis, including expression of the WEREWOLF (WER) and GLABRA2 (GL2) genes. Double mutant analysis reveal that wer and caprice (cpc) mutants, defective in core components of the epidermal patterning pathway, genetically interact with sab. This suggests that SAB may act on epidermal patterning upstream of WER and CPC. Hence, we provide evidence for a role of SAB in root epidermal patterning by affecting cell-fate stabilization. Our work opens the door for future studies addressing SAB-dependent functions of the cytoskeleton during root epidermal patterning.
Y1  - 2015
UR  - http://onlinelibrary.wiley.com/doi/10.1111/ppl.12257/epdf
U6  - https://doi.org/DOI: 10.1111/ppl.12257
VL  - 153
IS  - 3
SP  - 440
EP  - 453
ER  - 
TY  - JOUR
A1  - Stanislas, Thomas
A1  - Huser, Anke
A1  - Barbosa, Ines C. R.
A1  - Kiefer, Christian S.
A1  - Brackmann, Klaus
A1  - Pietra, Stefano
A1  - Gustavsson, Anna
A1  - Zourelidou, Melina
A1  - Schwechheimer, Claus
A1  - Grebe, Markus
T1  - Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity
JF  - Nature plants
N2  - Development of diverse multicellular organisms relies on coordination of single-cell polarities within the plane of the tissue layer (planar polarity). Cell polarity often involves plasma membrane heterogeneity generated by accumulation of specific lipids and proteins into membrane subdomains. Coordinated hair positioning along Arabidopsis root epidermal cells provides a planar polarity model in plants, but knowledge about the functions of proteo-lipid domains in planar polarity signalling remains limited. Here we show that Rho-of-plant (ROP) 2 and 6, phosphatidylinositol-4-phosphate 5-kinase 3 (PIP5K3), DYNAMIN-RELATED PROTEIN (DRP) 1A and DRP2B accumulate in a sterol-enriched, polar membrane domain during root hair initiation. DRP1A, DRP2B, PIP5K3 and sterols are required for planar polarity and the AGCVIII kinase D6 PROTEIN KINASE (D6PK) is a modulator of this process. D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-to-planar polarity switching into the polar, lipid-enriched domain just before hair formation, unravelling lipid-dependent D6PK localization during late planar polarity signalling.
Y1  - 2015
U6  - https://doi.org/10.1038/NPLANTS.2015.162
SN  - 2055-026X
SN  - 2055-0278
VL  - 1
IS  - 11
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Pietra, Stefano
A1  - Lang, Patricia
A1  - Grebe, Markus
T1  - SABRE is required for stabilization of root hair patterning in Arabidopsis thaliana
JF  - Physiologia plantarum
N2  - Patterned differentiation of distinct cell types is essential for the development of multicellular organisms. The root epidermis of Arabidopsis thaliana is composed of alternating files of root hair and non-hair cells and represents a model system for studying the control of cell-fate acquisition. Epidermal cell fate is regulated by a network of genes that translate positional information from the underlying cortical cell layer into a specific pattern of differentiated cells. While much is known about the genes of this network, new players continue to be discovered. Here we show that the SABRE (SAB) gene, known to mediate microtubule organization, anisotropic cell growth and planar polarity, has an effect on root epidermal hair cell patterning. Loss of SAB function results in ectopic root hair formation and destabilizes the expression of cell fate and differentiation markers in the root epidermis, including expression of the WEREWOLF (WER) and GLABRA2 (GL2) genes. Double mutant analysis reveal that wer and caprice (cpc) mutants, defective in core components of the epidermal patterning pathway, genetically interact with sab. This suggests that SAB may act on epidermal patterning upstream of WER and CPC. Hence, we provide evidence for a role of SAB in root epidermal patterning by affecting cell-fate stabilization. Our work opens the door for future studies addressing SAB-dependent functions of the cytoskeleton during root epidermal patterning.
Y1  - 2015
U6  - https://doi.org/10.1111/ppl.12257
SN  - 0031-9317
SN  - 1399-3054
VL  - 153
IS  - 3
SP  - 440
EP  - 453
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Kiefer, Christian S.
A1  - Claes, Andrea R.
A1  - Nzayisenga, Jean-Claude
A1  - Pietra, Stefano
A1  - Stanislas, Thomas
A1  - Hueser, Anke
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity
JF  - Development : Company of Biologists
N2  - The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.
KW  - AIP1
KW  - Arabidopsis
KW  - WEREWOLF
KW  - Actin
KW  - Patterning
KW  - Planar polarity
Y1  - 2015
U6  - https://doi.org/10.1242/dev.111013
SN  - 0950-1991
SN  - 1477-9129
VL  - 142
IS  - 1
SP  - 151
EP  - 161
PB  - Company of Biologists Limited
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Frescatada-Rosa, Marcia
A1  - Stanislas, Thomas
A1  - Backues, Steven K.
A1  - Reichardt, Ilka
A1  - Men, Shuzhen
A1  - Boutte, Yohann
A1  - Juergens, Gerd
A1  - Moritz, Thomas
A1  - Bednarek, Sebastian York
A1  - Grebe, Markus
T1  - High lipid order of Arabidopsis cell-plate membranes mediated by sterol and Dynamin-Related Protein 1A function
JF  - The plant journal
N2  - Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant cells is the membrane structure that separates daughter cells during somatic cytokinesis. Cell-plate formation in Arabidopsis relies on sterol- and DYNAMIN-RELATED PROTEIN1A (DRP1A)-dependent endocytosis. However, functional relationships between lipid membrane order or lipid packing and endocytic machinery components during eukaryotic cytokinesis have not been elucidated. Using ratiometric live imaging of lipid order-sensitive fluorescent probes, we show that the cell plate of Arabidopsis thaliana represents a dynamic, high lipid-order membrane domain. The cell-plate lipid order was found to be sensitive to pharmacological and genetic alterations of sterol composition. Sterols co-localize with DRP1A at the cell plate, and DRP1A accumulates in detergent-resistant membrane fractions. Modifications of sterol concentration or composition reduce cell-plate membrane order and affect DRP1A localization. Strikingly, DRP1A function itself is essential for high lipid order at the cell plate. Our findings provide evidence that the cell plate represents a high lipid-order domain, and pave the way to explore potential feedback between lipid order and function of dynamin-related proteins during cytokinesis.
KW  - membrane order
KW  - sterol
KW  - cytokinesis
KW  - DRP1A
KW  - Arabidopsis
Y1  - 2014
U6  - https://doi.org/10.1111/tpj.12674
SN  - 0960-7412
SN  - 1365-313X
VL  - 80
IS  - 5
SP  - 745
EP  - 757
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -