TY - JOUR A1 - Berger, Beatrice A1 - Baldermann, Susanne A1 - Ruppel, Silke T1 - The plant growth-promoting bacterium Kosakonia radicincitans improves fruit yield and quality of Solanum lycopersicum JF - Journal of the Science of Food and Agriculture N2 - BACKGROUNDProduction and the quality of tomato fruits have a strong economic relevance. Microorganisms such as the plant growth-promoting bacterium (PGPB) Kosakonia radicincitans (DSM 16656) have been demonstrated to improve shoot and root growth of young tomato plants, but data on yield increase and fruit quality by K. radicincitans are lacking. RESULTSThis study investigated how K. radicincitans affects tomato fruits. After inoculation of tomato seeds with K. radicincitans or a sodium chloride buffer control solution, stalk length, first flowering and the amount of ripened fruits produced by inoculated and non-inoculated plants were monitored over a period of 21 weeks. Inoculation of tomato seeds with K. radicincitans accelerated flowering and ripening of tomato fruits. Sugars, acidity, amino acids, volatile organic compounds and carotenoids in the fruits were also analyzed. CONCLUSIONIt was found that the PGPBK. radicincitans affected the amino acid, sugar and volatile composition of ripened fruits, contributing to a more pleasant-tasting fruit without forfeiting selected quality indicators. (c) 2017 Society of Chemical Industry KW - plant growth-promoting bacteria KW - Kosakonia radicincitans KW - fruit metabolites KW - Solanum lycopersicum Y1 - 2017 U6 - https://doi.org/10.1002/jsfa.8357 SN - 0022-5142 SN - 1097-0010 VL - 97 SP - 4865 EP - 4871 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Lisso, Janina A1 - Altmann, Thomas A1 - Müssig, Carsten T1 - Metabolic changes in fruits of the tomato d(x) mutant JF - Phytochemistry : an international journal of plant biochemistry KW - Solanum lycopersicum KW - Solanaceae KW - tomato KW - brassinosteroid KW - primary metabolism KW - fruit Y1 - 2006 U6 - https://doi.org/10.1016/j.phytochem.2006.07.008 SN - 0031-9422 VL - 67 IS - 20 SP - 2232 EP - 2238 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Errard, Audrey A1 - Ulrichs, Christian A1 - Kuehne, Stefan A1 - Mewis, Inga A1 - Drungowski, Mario A1 - Schreiner, Monika A1 - Baldermann, Susanne T1 - Single- versus multiple-pest infestation affects differently the Biochemistry of Tomato (Solanum lycopersicum 'Ailsa Craig') JF - Journal of agricultural and food chemistry : a publication of the American Chemical Society N2 - Tomato is susceptible to pest infestations by both spider mites and aphids. The effects of each individual pest on plants are known, whereas multiple-pest infestations have received little interest. We studied the effects of single-versus multiple-pest infestation by Tetranychus urticae and Myzus persicae on tomato biochemistry (Solanum lycopersicum) by combining a metabolomic approach and analyses of carotenoids using UHPLC-ToF-MS and volatiles using GC-MS. Plants responded differently to aphids and mites after 3 weeks of infestation, and a multiple infestation induced a specific metabolite composition in plants. In addition, we showed that volatiles emissions differed between the adaxial and abaxial leaf epidermes and identified compounds emitted particularly in response to a multiple infestation (cyclohexadecane, dodecane, aromadendrene, and beta-elemene). Finally, the carotenoid concentrations in leaves and stems were more affected by multiple than single infestations. Our study highlights and discusses the interplay of biotic stressors within the terpenoid metabolism. KW - spider mites KW - aphids KW - terpenoids KW - carotenoids KW - plant volatiles KW - systemic response KW - Solanaceae KW - Solanum lycopersicum Y1 - 2015 U6 - https://doi.org/10.1021/acs.jafc.5b03884 SN - 0021-8561 SN - 1520-5118 VL - 63 IS - 46 SP - 10103 EP - 10111 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Schwahn, Kevin A1 - de Souza, Leonardo Perez A1 - Fernie, Alisdair R. A1 - Tohge, Takayuki T1 - Metabolomics-assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade JF - Journal of integrative plant biology N2 - Steroidal glycoalkaloids (SGAs) are nitrogen-containing secondary metabolites of the Solanum species, which are known to have large chemical and bioactive diversity in nature. While recent effort and development on LC/MS techniques for SGA profiling have elucidated the main pathways of SGA metabolism in tomato, the problem of peak annotation still remains due to the vast diversity of chemical structure and similar on overlapping of chemical formula. Here we provide a case study of peak classification and annotation approach by integration of species and tissue specificities of SGA accumulation for provision of comprehensive pathways of SGA biosynthesis. In order to elucidate natural diversity of SGA biosynthesis, a total of 169 putative SGAs found in eight tomato accessions (Solanum lycopersicum, S. pimpinellifolium, S. cheesmaniae, S. chmielewskii, S. neorickii, S. peruvianum, S. habrochaites, S. pennellii) and four tissue types were used for correlation analysis. The results obtained in this study contribute annotation and classification of SGAs as well as detecting putative novel biosynthetic branch points. As such this represents a novel strategy for peak annotation for plant secondary metabolites. KW - Fruit ripening KW - glycoalkaloids KW - secondary metabolite KW - Solanum lycopersicum KW - tomato accessions Y1 - 2014 U6 - https://doi.org/10.1111/jipb.12274 SN - 1672-9072 SN - 1744-7909 VL - 56 IS - 9 SP - 864 EP - 875 PB - Wiley-Blackwell CY - Hoboken ER - TY - JOUR A1 - Rohrmann, Johannes A1 - Tohge, Takayuki A1 - Alba, Rob A1 - Osorio, Sonia A1 - Caldana, Camila A1 - McQuinn, Ryan A1 - Arvidsson, Samuel Janne A1 - van der Merwe, Margaretha J. A1 - Riano-Pachon, Diego Mauricio A1 - Müller-Röber, Bernd A1 - Fei, Zhangjun A1 - Nesi, Adriano Nunes A1 - Giovannoni, James J. A1 - Fernie, Alisdair R. T1 - Combined transcription factor profiling, microarray analysis and metabolite profiling reveals the transcriptional control of metabolic shifts occurring during tomato fruit development JF - The plant journal N2 - Maturation of fleshy fruits such as tomato (Solanum lycopersicum) is subject to tight genetic control. Here we describe the development of a quantitative real-time PCR platform that allows accurate quantification of the expression level of approximately 1000 tomato transcription factors. In addition to utilizing this novel approach, we performed cDNA microarray analysis and metabolite profiling of primary and secondary metabolites using GC-MS and LC-MS, respectively. We applied these platforms to pericarp material harvested throughout fruit development, studying both wild-type Solanum lycopersicum cv. Ailsa Craig and the hp1 mutant. This mutant is functionally deficient in the tomato homologue of the negative regulator of the light signal transduction gene DDB1 from Arabidopsis, and is furthermore characterized by dramatically increased pigment and phenolic contents. We choose this particular mutant as it had previously been shown to have dramatic alterations in the content of several important fruit metabolites but relatively little impact on other ripening phenotypes. The combined dataset was mined in order to identify metabolites that were under the control of these transcription factors, and, where possible, the respective transcriptional regulation underlying this control. The results are discussed in terms of both programmed fruit ripening and development and the transcriptional and metabolic shifts that occur in parallel during these processes. KW - transcription factor KW - Solanum lycopersicum KW - quantitative RT-PCR KW - microarray KW - metabolomics KW - fleshy fruit ripening Y1 - 2011 U6 - https://doi.org/10.1111/j.1365-313X.2011.04750.x SN - 0960-7412 VL - 68 IS - 6 SP - 999 EP - 1013 PB - Wiley-Blackwell CY - Malden ER -