TY  - GEN
A1  - Guljamow, Arthur
A1  - Barchewitz, Tino
A1  - Große, Rebecca
A1  - Timm, Stefan
A1  - Hagemann, Martin
A1  - Dittmann, Elke
T1  - Diel Variations of Extracellular Microcystin Influence the
Subcellular Dynamics of RubisCO in Microcystis aeruginosa
PCC 7806
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The ubiquitous freshwater cyanobacterium Microcystis is remarkably successful, showing a high tolerance against fluctuations in environmental conditions. It frequently forms dense blooms which can accumulate significant amounts of the hepatotoxin microcystin, which plays an extracellular role as an infochemical but also acts intracellularly by interacting with proteins of the carbon metabolism, notably with the CO2 fixing enzyme RubisCO. Here we demonstrate a direct link between external microcystin and its intracellular targets. Monitoring liquid cultures of Microcystis in a diel experiment revealed fluctuations in the extracellular microcystin content that correlate with an increase in the binding of microcystin to intracellular proteins. Concomitantly, reversible relocation of RubisCO from the cytoplasm to the cell’s periphery was observed. These variations in RubisCO localization were especially pronounced with cultures grown at higher cell densities. We replicated these effects by adding microcystin externally to cultures grown under continuous light. Thus, we propose that microcystin may be part of a fast response to conditions of high light and low carbon that contribute to the metabolic flexibility and the success of Microcystis in the field.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1154 
KW  - cyanobacterial bloom
KW  - Microcystis
KW  - microcystin
KW  - RubisCO
KW  - extracellular signaling
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-521287
SN  - 1866-8372
IS  - 1154
ER  - 
TY  - GEN
A1  - des Aulnois, Maxime Georges
A1  - Réveillon, Damien
A1  - Robert, Elise
A1  - Caruana, Amandine
A1  - Briand, Enora
A1  - Guljamow, Arthur
A1  - Dittmann, Elke
A1  - Amzil, Zouher
A1  - Bormans, Myriam
T1  - Salt shock responses of Microcystis revealed through physiological, transcript, and metabolomic analyses
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The transfer of Microcystis aeruginosa from freshwater to estuaries has been described worldwide and salinity is reported as the main factor controlling the expansion of M. aeruginosa to coastal environments. Analyzing the expression levels of targeted genes and employing both targeted and non-targeted metabolomic approaches, this study investigated the effect of a sudden salt increase on the physiological and metabolic responses of two toxic M. aeruginosa strains separately isolated from fresh and brackish waters, respectively, PCC 7820 and 7806. Supported by differences in gene expressions and metabolic profiles, salt tolerance was found to be strain specific. An increase in salinity decreased the growth of M. aeruginosa with a lesser impact on the brackish strain. The production of intracellular microcystin variants in response to salt stress correlated well to the growth rate for both strains. Furthermore, the release of microcystins into the surrounding medium only occurred at the highest salinity treatment when cell lysis occurred. This study suggests that the physiological responses of M. aeruginosa involve the accumulation of common metabolites but that the intraspecific salt tolerance is based on the accumulation of specific metabolites. While one of these was determined to be sucrose, many others remain to be identified. Taken together, these results provide evidence that M. aeruginosa is relatively salt tolerant in the mesohaline zone and microcystin (MC) release only occurs when the capacity of the cells to deal with salt increase is exceeded.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1130 
KW  - Microcystis aeruginosa
KW  - microcystin
KW  - salt stress
KW  - metabolomic
KW  - transcript
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-472405
SN  - 1866-8372
IS  - 1130
ER  - 
TY  - GEN
A1  - Mantzouki, Evanthia
A1  - Lürling, Miquel
A1  - Fastner, Jutta
A1  - Domis, Lisette Nicole de Senerpont
A1  - Wilk-Woźniak, Elżbieta
A1  - Koreiviene, Judita
A1  - Seelen, Laura
A1  - Teurlincx, Sven
A1  - Verstijnen, Yvon
A1  - Krztoń, Wojciech
A1  - Walusiak, Edward
A1  - Karosienė, Jūratė
A1  - Kasperovičienė, Jūratė
A1  - Savadova, Ksenija
A1  - Vitonytė, Irma
A1  - Cillero-Castro, Carmen
A1  - Budzyńska, Agnieszka
A1  - Goldyn, Ryszard
A1  - Kozak, Anna
A1  - Rosińska, Joanna
A1  - Szeląg-Wasielewska, Elżbieta
A1  - Domek, Piotr
A1  - Jakubowska-Krepska, Natalia
A1  - Kwasizur, Kinga
A1  - Messyasz, Beata
A1  - Pełechata, Aleksandra
A1  - Pełechaty, Mariusz
A1  - Kokocinski, Mikolaj
A1  - García-Murcia, Ana
A1  - Real, Monserrat
A1  - Romans, Elvira
A1  - Noguero-Ribes, Jordi
A1  - Duque, David Parreño
A1  - Fernández-Morán, Elísabeth
A1  - Karakaya, Nusret
A1  - Häggqvist, Kerstin
A1  - Beklioğlu, Meryem
A1  - Filiz, Nur
A1  - Levi, Eti E.
A1  - Iskin, Uğur
A1  - Bezirci, Gizem
A1  - Tavşanoğlu, Ülkü Nihan
A1  - Özhan, Koray
A1  - Gkelis, Spyros
A1  - Panou, Manthos
A1  - Fakioglu, Özden
A1  - Avagianos, Christos
A1  - Kaloudis, Triantafyllos
A1  - Çelik, Kemal
A1  - Yilmaz, Mete
A1  - Marcé, Rafael
A1  - Catalán, Nuria
A1  - Bravo, Andrea G.
A1  - Buck, Moritz
A1  - Colom-Montero, William
A1  - Mustonen, Kristiina
A1  - Pierson, Don
A1  - Yang, Yang
A1  - Raposeiro, Pedro M.
A1  - Gonçalves, Vítor
A1  - Antoniou, Maria G.
A1  - Tsiarta, Nikoletta
A1  - McCarthy, Valerie
A1  - Perello, Victor C.
A1  - Feldmann, Tõnu
A1  - Laas, Alo
A1  - Panksep, Kristel
A1  - Tuvikene, Lea
A1  - Gagala, Ilona
A1  - Mankiewicz-Boczek, Joana
A1  - Yağcı, Meral Apaydın
A1  - Çınar, Şakir
A1  - Çapkın, Kadir
A1  - Yağcı, Abdulkadir
A1  - Cesur, Mehmet
A1  - Bilgin, Fuat
A1  - Bulut, Cafer
A1  - Uysal, Rahmi
A1  - Obertegger, Ulrike
A1  - Boscaini, Adriano
A1  - Flaim, Giovanna
A1  - Salmaso, Nico
A1  - Cerasino, Leonardo
A1  - Richardson, Jessica
A1  - Visser, Petra M.
A1  - Verspagen, Jolanda M. H.
A1  - Karan, Tünay
A1  - Soylu, Elif Neyran
A1  - Maraşlıoğlu, Faruk
A1  - Napiórkowska-Krzebietke, Agnieszka
A1  - Ochocka, Agnieszka
A1  - Pasztaleniec, Agnieszka
A1  - Antão-Geraldes, Ana M.
A1  - Vasconcelos, Vitor
A1  - Morais, João
A1  - Vale, Micaela
A1  - Köker, Latife
A1  - Akçaalan, Reyhan
A1  - Albay, Meriç
A1  - Maronić, Dubravka Špoljarić
A1  - Stević, Filip
A1  - Pfeiffer, Tanja Žuna
A1  - Fonvielle, Jeremy Andre
A1  - Straile, Dietmar
A1  - Rothhaupt, Karl-Otto
A1  - Hansson, Lars-Anders
A1  - Urrutia-Cordero, Pablo
A1  - Bláha, Luděk
A1  - Geriš, Rodan
A1  - Fránková, Markéta
A1  - Koçer, Mehmet Ali Turan
A1  - Alp, Mehmet Tahir
A1  - Remec-Rekar, Spela
A1  - Elersek, Tina
A1  - Triantis, Theodoros
A1  - Zervou, Sevasti-Kiriaki
A1  - Hiskia, Anastasia
A1  - Haande, Sigrid
A1  - Skjelbred, Birger
A1  - Madrecka, Beata
A1  - Nemova, Hana
A1  - Drastichova, Iveta
A1  - Chomova, Lucia
A1  - Edwards, Christine
A1  - Sevindik, Tuğba Ongun
A1  - Tunca, Hatice
A1  - Önem, Burçin
A1  - Aleksovski, Boris
A1  - Krstić, Svetislav
A1  - Vucelić, Itana Bokan
A1  - Nawrocka, Lidia
A1  - Salmi, Pauliina
A1  - Machado-Vieira, Danielle
A1  - Oliveira, Alinne Gurjão De
A1  - Delgado-Martín, Jordi
A1  - García, David
A1  - Cereijo, Jose Luís
A1  - Gomà, Joan
A1  - Trapote, Mari Carmen
A1  - Vegas-Vilarrúbia, Teresa
A1  - Obrador, Biel
A1  - Grabowska, Magdalena
A1  - Karpowicz, Maciej
A1  - Chmura, Damian
A1  - Úbeda, Bárbara
A1  - Gálvez, José Ángel
A1  - Özen, Arda
A1  - Christoffersen, Kirsten Seestern
A1  - Warming, Trine Perlt
A1  - Kobos, Justyna
A1  - Mazur-Marzec, Hanna
A1  - Pérez-Martínez, Carmen
A1  - Ramos-Rodríguez, Eloísa
A1  - Arvola, Lauri
A1  - Alcaraz-Párraga, Pablo
A1  - Toporowska, Magdalena
A1  - Pawlik-Skowronska, Barbara
A1  - Niedźwiecki, Michał
A1  - Pęczuła, Wojciech
A1  - Leira, Manel
A1  - Hernández, Armand
A1  - Moreno-Ostos, Enrique
A1  - Blanco, José María
A1  - Rodríguez, Valeriano
A1  - Montes-Pérez, Jorge Juan
A1  - Palomino, Roberto L.
A1  - Rodríguez-Pérez, Estela
A1  - Carballeira, Rafael
A1  - Camacho, Antonio
A1  - Picazo, Antonio
A1  - Rochera, Carlos
A1  - Santamans, Anna C.
A1  - Ferriol, Carmen
A1  - Romo, Susana
A1  - Soria, Juan Miguel
A1  - Dunalska, Julita
A1  - Sieńska, Justyna
A1  - Szymański, Daniel
A1  - Kruk, Marek
A1  - Kostrzewska-Szlakowska, Iwona
A1  - Jasser, Iwona
A1  - Žutinić, Petar
A1  - Udovič, Marija Gligora
A1  - Plenković-Moraj, Anđelka
A1  - Frąk, Magdalena
A1  - Bańkowska-Sobczak, Agnieszka
A1  - Wasilewicz, Michał
A1  - Özkan, Korhan
A1  - Maliaka, Valentini
A1  - Kangro, Kersti
A1  - Grossart, Hans-Peter
A1  - Paerl, Hans W.
A1  - Carey, Cayelan C.
A1  - Ibelings, Bas W.
T1  - Temperature effects explain continental scale distribution of cyanobacterial toxins
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1105 
KW  - microcystin
KW  - anatoxin
KW  - cylindrospermopsin
KW  - temperature
KW  - direct effects
KW  - indirect effects
KW  - spatial distribution
KW  - European Multi Lake Survey
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427902
SN  - 1866-8372
IS  - 1105
ER  -