TY  - JOUR
A1  - Vignon-Zellweger, Nicolas
A1  - Rahnenführer, Jan
A1  - Theuring, Franz
A1  - Hocher, Berthold
T1  - Analysis of cardiac and renal endothelin receptors by in situ hybridization in mice
JF  - Clinical laboratory : the peer reviewed journal for clinical laboratories and laboratories related to blood transfusion
N2  - Background: Endothelin-1 (ET-1) is a multifunctional peptide, which is implicated in the renal and cardiac physicology as well as in many pathologies of these systems. ET-1's actions take place after the activation of two receptors: ETA and ETB. The expression of these receptors may be modulated during the pathologic process. The analysis of the distribution and level of expression of the receptors in animal models is therefore crucial.
 Methods: We developed a protocol for non-radioactive in situ hybridization for the mRNA of the two endothelin receptors on paraffin-embedded tissue using digoxigenin-labeled RNA probes.
 Results: In heart and kidney, the staining was reliable and specific. In a mouse model for endothelin/nitric oxide imbalance, cardiac ETB expression was reduced. The distribution of the receptors was in accordance with the actual knowledge. Differences in cell specific expression are discussed.
 Conclusions: We developed a protocol for the in situ hybridization of the endothelin receptors in mice. Given that the endothelin system is implicated in the development of many diseases, we believe that this protocol may be useful for a number of future preclinical studies.
KW  - Endothelin-1
KW  - endothelin receptors
KW  - in situ hybridization
KW  - mouse
Y1  - 2012
U6  - https://doi.org/10.7754/Clin.Lab.2012.120216
SN  - 1433-6510
VL  - 58
IS  - 9-10
SP  - 939
EP  - 949
PB  - Clin Lab Publ., Verl. Klinisches Labor
CY  - Heidelberg
ER  -