TY  - JOUR
A1  - Lang, Ingo
A1  - Walz, Bernd
T1  - Dopamine stimulates salivary duct cells in the cockroach Pertiplaneta americana
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Lang, Ingo
A1  - Walz, Bernd
T1  - Dopamine-induced epithelial K+ and Na+ movements in the salivary ducts of Periplaneta americana
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Lang, Ingo
A1  - Walz, Bernd
T1  - Dye-coupling between cells of the salivary glands in the cockroach Periplaneta americana
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Rein, Julia
A1  - Zimmermann, Bernhard
A1  - Hille, Carsten
A1  - Lang, Ingo
A1  - Walz, Bernd
A1  - Baumann, Otto
T1  - Fluorescence measurements of serotonin-induced V-ATPase-dependent pH changes at the luminal surface in salivary glands of the blowfly Calliphora vicina
N2  - Secretion in blowfly salivary glands is induced by the neurohormone serotonin and powered by a vacuolar-type H+- ATPase (V-ATPase) located in the apical membrane of the secretory cells. We have established a microfluorometric method for analysing pH changes at the luminal surface of the secretory epithelial cells by using the fluorescent dye 5-N- hexadecanoyl-aminofluorescein (HAF). After injection of HAF into the lumen of the tubular salivary gland, the fatty acyl chain of the dye molecule partitions into the outer leaflet of the plasma membrane and its pH-sensitive fluorescent moiety is exposed at the cell surface. Confocal imaging has confirmed that HAF distributes over the entire apical membrane of the secretory cells and remains restricted to this membrane domain. Ratiometric analysis of HAF fluorescence demonstrates that serotonin leads to a reversible dose-dependent acidification at the luminal surface. Inhibition by concanamycin A confirms that the serotonin-induced acidification at the luminal surface is due to H+ transport across the apical membrane via V-ATPase. Measurements with pH-sensitive microelectrodes corroborate a serotonin-induced luminal acidification and demonstrate that luminal pH decreases by about 0.4 pH units at saturating serotonin concentrations. We conclude that ratiometric measurements of HAF fluorescence provide an elegant method for monitoring V-ATPase-dependent H+ transport in the blowfly salivary gland in vivo and for analysing the spatiotemporal pattern of pH changes at the luminal surface
Y1  - 2006
UR  - http://jeb.biologists.org/
U6  - https://doi.org/10.1242/Jeb.02187
SN  - 0022-0949
ER  - 
TY  - JOUR
A1  - Schweigel, Monika
A1  - Lang, Ingo
A1  - Martens, Holger
T1  - Mg2+transport of sheep rumen epithelium : evidence for an electrodiffusive uptake mechanism
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Lang, Ingo
A1  - Martens, Holger
T1  - Na transport in sheep rumen is modulated by voltage-dependent cation conductance in apical membrane
Y1  - 1999
ER  -