TY  - JOUR
A1  - Han, Xiao Xia
A1  - Li, Junbo
A1  - Öner, Ibrahim Halil
A1  - Zhao, Bing
A1  - Leimkühler, Silke
A1  - Hildebrandt, Peter
A1  - Weidinger, Inez M.
T1  - Nickel electrodes as a cheap and versatile platform for studying structure and function of immobilized redox proteins
JF  - Analytica chimica acta : an international journal devoted to all branches of analytical chemistry
N2  - Practical use of many bioelectronic and bioanalytical devices is limited by the need of expensive materials and time consuming fabrication. Here we demonstrate the use of nickel electrodes as a simple and cheap solid support material for bioelectronic applications. The naturally nanostructured electrodes showed a surprisingly high electromagnetic surface enhancement upon light illumination such that immobilization and electron transfer reactions of the model redox proteins cytochrome b(5) (Cyt b(5)) and cytochrome c (Cyt c) could be followed via surface enhanced resonance Raman spectroscopy. It could be shown that the nickel surface, when used as received, promotes a very efficient binding of the proteins upon preservation of their native structure. The immobilized redox proteins could efficiently exchange electrons with the electrode and could even act as an electron relay between the electrode and solubilized myoglobin. Our results open up new possibility for nickel electrodes as an exceptional good support for bioelectronic devices and biosensors on the one hand and for surface enhanced spectroscopic investigations on the other hand. (C) 2016 Elsevier B.V. All rights reserved.
KW  - Ni electrodes
KW  - Redox proteins
KW  - Surface enhanced Raman spectroscopy
KW  - Electron relay
KW  - Biocompatibility
KW  - Electron transfer
Y1  - 2016
U6  - https://doi.org/10.1016/j.aca.2016.08.053
SN  - 0003-2670
SN  - 1873-4324
VL  - 941
SP  - 35
EP  - 40
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Cazelles, R.
A1  - Lalaoui, N.
A1  - Hartmann, Tobias
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Antonietti, Markus
A1  - Cosnier, S.
T1  - Ready to use bioinformatics analysis as a tool to predict immobilisation strategies for protein direct electron transfer (DET)
JF  - Polymer : the international journal for the science and technology of polymers
KW  - Bioinformatic
KW  - Bioelectrocatalysis
KW  - Electron transfer
KW  - Dehydrogenase
KW  - Nicotinamide
Y1  - 2016
U6  - https://doi.org/10.1016/j.bios.2016.04.078
SN  - 0956-5663
SN  - 1873-4235
VL  - 85
SP  - 90
EP  - 95
PB  - Elsevier
CY  - Oxford
ER  -