TY  - JOUR
A1  - Oloya, Benson
A1  - Namukobe, Jane
A1  - Heydenreich, Matthias
A1  - Ssengooba, Willy
A1  - Schmidt, Bernd
A1  - Byamukama, Robert
T1  - Antimycobacterial activity of the extract and isolated compounds from the stem bark of Zanthoxylum leprieurii Guill. and Perr.
JF  - Natural product communications : an international journal for communications and reviews
N2  - Zanthoxylum leprieurii Guill. and Perr. (Rutaceae) stem bark is used locally in Uganda for treating tuberculosis (TB) and cough-related infections. Lupeol (1), sesamin (2), trans-fagaramide (3), arnottianamide (4), (S)-marmesinin (5), and hesperidin (6) were isolated from the chloroform/methanol (1:1) extract of Z. leprieurii stem bark. Their structures were elucidated using spectroscopic techniques and by comparison with literature data. Furthermore, the extract and isolated compounds were subjected to antimycobacterial activity. The extract exhibited moderate activity against the susceptible (H(37)Rv) TB strain, but weak activity against the multidrug resistant (MDR)-TB strain with minimum inhibitory concentrations (MICs) of 586.0 and 1172.0 mu g/mL, respectively. Compound 3 (trans-fagaramide) showed significant antimycobacterial activity against the susceptible (H(37)Rv) TB strain (MIC 6 mu g/mL), but moderate activity against the MDR-TB strain (MIC 12.2 mu g/mL). Compounds 2, 5, 6, and 1 showed moderate activities against the susceptible (H(37)Rv) strain (MIC 12.2-98.0 mu g/mL) and moderate to weak activities against the MDR-TB strain (MIC 24.4-195.0 mu g/mL). This study reports for the first time the isolation of compounds 1 to 6 from the stem bark of Z leprieurii. trans-Fagaramide (3) may present a vital template in pursuit of novel and highly effective TB drugs.
KW  - tuberculosis
KW  - Zanthoxylum leprieurii
KW  - antimycobacterial activity
KW  - trans-fagaramide
KW  - alkaloid
Y1  - 2021
U6  - https://doi.org/10.1177/1934578X211035851
SN  - 1934-578X
SN  - 1555-9475
VL  - 16
IS  - 8
PB  - Sage Publ.
CY  - Thousand Oaks
ER  - 
TY  - JOUR
A1  - Induli, Martha
A1  - Cheloti, Michael
A1  - Wasuna, Antonina
A1  - Wekesa, Ingrid
A1  - Wanjohi, John M.
A1  - Byamukama, Robert
A1  - Heydenrich, Matthias
A1  - Makayoto, Moses
A1  - Yenesew, Abiy
T1  - Naphthoquinones from the roots of Aloe secundiflora
JF  - Phytochemistry letters
N2  - Two new naphthoquinones, 5-hydroxy-3,6-dimethoxy-2-methylnaphthalene-1,4-dione and 5,8-dihydroxy-3-methoxy-2-methylnaphthalene-1,4-dione, were isolated from the roots of Aloe secundiflora together with the known compounds chrysophanol, helminthosporin, isoxanthorin, ancistroquinone C, aloesaponarins I and II, aloesaponols I and II, laccaic acid D methyl ester and asphodelin. The structures were elucidated based on spectroscopic evidence. This appears to be the first report on the occurrence of naphthoquinones in the genus Aloe. Aloesaponarin I and 5-hydroxy-3,6-dimethoxy-2-methylnaphthalene-1,4-dione showed anti-bacterial activity against Mycobacterium tuberculosis with MIC values of 21-23 mu g/mL in the Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA); 5-hydroxy-3,6-dimethoxy-2-methylnaphthalene-1,4-dione also showed cytotoxicity against the Vero cell line (IC50 = 10.2 mu g/mL).
KW  - Aloe secundiflora
KW  - Asphodelaceae
KW  - Roots
KW  - Naphthoquinone
KW  - 5-Hydroxy-3,6-dimethoxy-2-methylnaphthalene-1,4-dione 5,8-Dihydroxy-3-methoxy-2-methylnaphthalene-1,4-dione
KW  - Anthraquinone
KW  - Aloesaponarin I
KW  - Mycobacterium tuberculosis
Y1  - 2012
U6  - https://doi.org/10.1016/j.phytol.2012.04.014
SN  - 1874-3900
VL  - 5
IS  - 3
SP  - 506
EP  - 509
PB  - Elsevier
CY  - Amsterdam
ER  -