TY  - JOUR
A1  - Yarman, Aysu
T1  - Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase
JF  - Turkish journal of chemistry
N2  - For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase Kor by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R 2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
KW  - Molecularly imprinted polymers
KW  - biomimetic sensors
KW  - tyrosinase
KW  - electropolymerization
KW  - scopoletin
KW  - ophenylenediamine
Y1  - 2017
U6  - https://doi.org/10.3906/kim-1708-68
SN  - 1300-0527
VL  - 42
IS  - 2
SP  - 346
EP  - 354
PB  - Türkiye Bilimsel ve Teknik Araştırma Kurumu
CY  - Ankara
ER  -