TY  - JOUR
A1  - Berry, Scott
A1  - Rosa, Stefanie
A1  - Howard, Martin
A1  - Buhler, Marc
A1  - Dean, Caroline
T1  - Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression
JF  - Genes & Development
N2  - Epigenetic maintenance of gene repression is essential for development. Polycomb complexes are central to this memory, but many aspects of the underlying mechanism remain unclear. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) binds Polycomb-deposited H3K27me3 and is required for repression of many Polycomb target genes in Arabidopsis. Here we show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region. By independently perturbing the RNA-binding hinge region and H3K27me3 (trimethylation of histone H3 at Lys27) recognition, we found that both facilitate LHP1 localization and H3K27me3 maintenance. Disruption of the RNAbinding hinge region also prevented formation of subnuclear foci, structures potentially important for epigenetic repression.
KW  - chromatin
KW  - epigenetics
KW  - plant biology
KW  - Polycomb
KW  - RNA
Y1  - 2017
U6  - https://doi.org/10.1101/gad.305227.117
SN  - 0890-9369
SN  - 1549-5477
VL  - 31
SP  - 2115
EP  - 2120
PB  - Cold Spring Harbor Laboratory Press
CY  - Cold Spring Harbor, NY
ER  -