TY  - JOUR
A1  - Neugart, Susanne
A1  - Wiesner-Reinhold, Melanie
A1  - Frede, Katja
A1  - Jander, Elisabeth
A1  - Homann, Thomas
A1  - Rawel, Harshadrai Manilal
A1  - Schreiner, Monika
A1  - Baldermann, Susanne
T1  - Effect of Solid Biological Waste Compost on the Metabolite Profile of Brassica rapa ssp chinensis
JF  - Frontiers in plant science : FPLS
N2  - Large quantities of biological waste are generated at various steps within the food production chain and a great utilization potential for this solid biological waste exists apart from the current main usage for the feedstuff sector. It remains unclear how the usage of biological waste as compost modulates plant metabolites. We investigated the effect of biological waste of the processing of coffee, aronia, and hop added to soil on the plant metabolite profile by means of liquid chromatography in pak choi sprouts. Here we demonstrate that the solid biological waste composts induced specific changes in the metabolite profiles and the changes are depending on the type of the organic residues and its concentration in soil. The targeted analysis of selected plant metabolites, associated with health beneficial properties of the Brassicaceae family, revealed increased concentrations of carotenoids (up to 3.2-fold) and decreased amounts of glucosinolates (up to 4.7-fold) as well as phenolic compounds (up to 1.5-fold).
KW  - metabolite profiling
KW  - LC/MS
KW  - pak choi
KW  - carotenoids
KW  - phenolic compounds
KW  - glucosinolates
Y1  - 2018
U6  - https://doi.org/10.3389/fpls.2018.00305
SN  - 1664-462X
VL  - 9
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Watanabe, Mutsumi
A1  - Tohge, Takayuki
A1  - Balazadeh, Salma
A1  - Erban, Alexander
A1  - Giavalisco, Patrick
A1  - Kopka, Joachim
A1  - Mueller-Roeber, Bernd
A1  - Fernie, Alisdair R.
A1  - Hoefgen, Rainer
T1  - Comprehensive Metabolomics Studies of Plant Developmental Senescence
JF  - Plant Senescence: Methods and Protocols
N2  - Leaf senescence is an essential developmental process that involves diverse metabolic changes associated with degradation of macromolecules allowing nutrient recycling and remobilization. In contrast to the significant progress in transcriptomic analysis of leaf senescence, metabolomics analyses have been relatively limited. A broad overview of metabolic changes during leaf senescence including the interactions between various metabolic pathways is required to gain a better understanding of the leaf senescence allowing to link transcriptomics with metabolomics and physiology. In this chapter, we describe how to obtain comprehensive metabolite profiles and how to dissect metabolic shifts during leaf senescence in the model plant Arabidopsis thaliana. Unlike nucleic acid analysis for transcriptomics, a comprehensive metabolite profile can only be achieved by combining a suite of analytic tools. Here, information is provided for measurements of the contents of chlorophyll, soluble proteins, and starch by spectrophotometric methods, ions by ion chromatography, thiols and amino acids by HPLC, primary metabolites by GC/TOF-MS, and secondary metabolites and lipophilic metabolites by LC/ESI-MS. These metabolite profiles provide a rich catalogue of metabolic changes during leaf senescence, which is a helpful database and blueprint to be correlated to future studies such as transcriptome and proteome analyses, forward and reverse genetic studies, or stress-induced senescence studies.
KW  - Senescence
KW  - Metabolomics
KW  - Arabidopsis
KW  - GC/MS
KW  - LC/MS
KW  - HPLC
KW  - IC
Y1  - 2018
SN  - 978-1-4939-7672-0
SN  - 978-1-4939-7670-6
U6  - https://doi.org/10.1007/978-1-4939-7672-0_28
SN  - 1064-3745
SN  - 1940-6029
VL  - 1744
SP  - 339
EP  - 358
PB  - Humana Press
CY  - Totowa
ER  -