TY  - JOUR
A1  - Neugart, Susanne
A1  - Wiesner-Reinhold, Melanie
A1  - Frede, Katja
A1  - Jander, Elisabeth
A1  - Homann, Thomas
A1  - Rawel, Harshadrai Manilal
A1  - Schreiner, Monika
A1  - Baldermann, Susanne
T1  - Effect of Solid Biological Waste Compost on the Metabolite Profile of Brassica rapa ssp chinensis
JF  - Frontiers in plant science : FPLS
N2  - Large quantities of biological waste are generated at various steps within the food production chain and a great utilization potential for this solid biological waste exists apart from the current main usage for the feedstuff sector. It remains unclear how the usage of biological waste as compost modulates plant metabolites. We investigated the effect of biological waste of the processing of coffee, aronia, and hop added to soil on the plant metabolite profile by means of liquid chromatography in pak choi sprouts. Here we demonstrate that the solid biological waste composts induced specific changes in the metabolite profiles and the changes are depending on the type of the organic residues and its concentration in soil. The targeted analysis of selected plant metabolites, associated with health beneficial properties of the Brassicaceae family, revealed increased concentrations of carotenoids (up to 3.2-fold) and decreased amounts of glucosinolates (up to 4.7-fold) as well as phenolic compounds (up to 1.5-fold).
KW  - metabolite profiling
KW  - LC/MS
KW  - pak choi
KW  - carotenoids
KW  - phenolic compounds
KW  - glucosinolates
Y1  - 2018
U6  - https://doi.org/10.3389/fpls.2018.00305
SN  - 1664-462X
VL  - 9
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Ali, Mostafa
A1  - Homann, Thomas
A1  - Kreisel, Janka
A1  - Khalil, Mahmoud
A1  - Puhlmann, Ralf
A1  - Kruse, Hans-Peter
A1  - Rawel, Harshadrai Manilal
T1  - Characterization and modeling of the interactions between coffee storage proteins and phenolic compounds
JF  - Journal of agricultural and food chemistry : a publication of the American Chemical Society
N2  - This study addresses the interactions of coffee storage proteins with coffee-specific phenolic compounds. Protein profiles, of Coffea arabica and Coffea canephora (var robusta) were compared. Major Phenolic compounds were extracted and analyzed with appropriate methods. The polyphenol-protein interactions during protein extraction have been addressed by different analytical setups [reversed-phase high-performance liquid chromatography (RP-HPLC), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS), and Trolox equivalent antioxidant capacity (TEAC) assays], with focus directed toward identification of covalent adduct formation. The results indicate that C. arabica proteins are more susceptible to these interactions and the polyphenol oxidase activity seems to be a crucial factor for the formation of these addition products. A tentative allocation of the modification type and site in the protein has been attempted. Thus, the first available in silico modeling of modified coffee proteins is reported. The extent of these modifications may contribute to the structure and function of "coffee melanoidins" and are discussed in the context of coffee flavor formation.
KW  - Coffee beans
KW  - storage proteins
KW  - phenolic compounds
KW  - antioxidants
KW  - protein-phenol interactions
KW  - modeling
Y1  - 2012
U6  - https://doi.org/10.1021/jf303372a
SN  - 0021-8561
VL  - 60
IS  - 46
SP  - 11601
EP  - 11608
PB  - American Chemical Society
CY  - Washington
ER  -