TY  - JOUR
A1  - Rohn, Isabelle
A1  - Kroepfl, Nina
A1  - Bornhorst, Julia
A1  - Kühnelt, Doris
A1  - Schwerdtle, Tanja
T1  - Side-directed transfer and presystemic metabolism of selenoneine in a human intestinal barrier model
JF  - Molecular nutrition & food research : bioactivity, chemistry, immunology, microbiology, safety, technology
N2  - Scope: Selenoneine, a recently discovered selenium (Se) species mainly present in marine fish, is the Se analogue of ergothioneine, a sulfur-containing purported antioxidant. Although similar properties have been proposed for selenoneine, data on its relevance to human health are yet scarce. Here, the transfer and presystemic metabolism of selenoneine in an in vitro model of the human intestinal barrier are investigated. Methods and results: Selenoneine and the reference species Se-methylselenocysteine (MeSeCys) and selenite are applied to the Caco-2 intestinal barrier model. Selenoneine is transferred in higher amounts, but with similar kinetics as selenite, while MeSeCys shows the highest permeability. In contrast to the reference species, transfer of selenoneine is directed toward the blood side. Cellular Se contents demonstrate that selenoneine is efficiently taken up by Caco-2 cells. Moreover, HPLC/MS-based Se speciation studies reveal a partial metabolism to Se-methylselenoneine, a metabolite previously detected in human blood and urine. Conclusions: Selenoneine is likely to pass the intestinal barrier via transcellular, carrier-mediated transport, is highly bioavailable to Caco-2 cells and undergoes metabolic transformations. Therefore, further studies are needed to elucidate its possible health effects and to characterize the metabolism of selenoneine in humans.
KW  - bioavailability
KW  - Caco-2 intestinal barrier model
KW  - presystemic metabolism
KW  - selenoneine
KW  - Se-methylselenoneine
Y1  - 2019
U6  - https://doi.org/10.1002/mnfr.201900080
SN  - 1613-4125
SN  - 1613-4133
VL  - 63
IS  - 12
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Islam, Khan Shaiful
A1  - Khalil, Mahmoud
A1  - Männer, K.
A1  - Raila, Jens
A1  - Rawel, Harshadrai Manilal
A1  - Zentek, J.
A1  - Schweigert, Florian J.
T1  - Effect of dietary alpha-tocopherol on the bioavailability of lutein in laying hen
JF  - Journal of animal physiology and animal nutrition
N2  - Lutein and its isomer zeaxanthin have gained considerable interest as possible nutritional ingredient in the prevention of age-related macular degeneration (AMD) in humans. Egg yolk is a rich source of these carotenoids. As an oxidative sensitive component, antioxidants such as -tocopherol (T) might contribute to an improved accumulation in egg yolk. To test this, chickens were fed lutein esters (LE) with and without -tocopherol as an antioxidant. After depletion on a wheat-soya bean-based lutein-poor diet for 21days, laying hens (n=42) were equally divided into three groups and fed the following diets for 21days: control (basal diet), a LE group (40mg LE/kg feed) and LE+T group (40mg LE plus 100mg T/kg feed). Eggs and blood were collected periodically. Carotenoids and -tocopherol in yolk and blood plasma were determined by HPLC. Egg yolk was also analysed for total carotenoids using a one-step spectrophotometric method (iCheck(())). Lutein, zeaxanthin, -tocopherol and total carotenoids in egg yolk were highest after 14days of feeding and decreased slightly afterwards. At the end of the trial, eggs of LE+T group contained higher amount of lutein (13.72), zeaxanthin (0.65), -tocopherol (297.40) and total carotenoids (21.6) compared to the LE group (10.96, 0.55, 205.20 and 18.0mg/kg, respectively, p<0.05). Blood plasma values of LE+T group contain higher lutein (1.3), zeaxanthin (0.06) and tocopherol (20.1) compared to LE group (1.02, 0.04 and 14.90mg/l, respectively, p<0.05). In conclusion, dietary -tocopherol enhances bioavailability of lutein reflecting higher content in egg yolk and blood plasma. Improved bioavailability might be due to increased absorption of lutein in the presence of tocopherol and/or a greater stability of lutein/zeaxanthin due to the presence of -tocopherol as an antioxidant.
KW  - carotenoids
KW  - tocopherol
KW  - egg yolk
KW  - bioavailability
KW  - HPLC
KW  - iCheck
Y1  - 2016
U6  - https://doi.org/10.1111/jpn.12464
SN  - 0931-2439
SN  - 1439-0396
VL  - 100
SP  - 868
EP  - 875
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -