TY  - JOUR
A1  - Purba, Talveen S.
A1  - Brunken, Lars
A1  - Peake, Michael
A1  - Shahmalak, Asim
A1  - Chaves, Asuncion
A1  - Poblet, Enrique
A1  - Ceballos, Laura
A1  - Gandarillas, Alberto
A1  - Pausa, Ralf
T1  - Characterisation of cell cycle arrest and terminal differentiation in a maximally proliferative human epithelial tissue: Lessons from the human hair follicle matrix
JF  - European journal of cell biology
N2  - Human hair follicle (HF) growth and hair shaft formation require terminal differentiation-associated cell cycle arrest of highly proliferative matrix keratinocytes. However, the regulation of this complex event remains unknown. CIP/KIP family member proteins (p21(CIP1), p27(KIP1) and p57(KIP2)) regulate cell cycle progression/arrest, endoreplication, differentiation and apoptosis. Since they have not yet been adequately characterized in the human HF, we asked whether and where CIP/KIP proteins localise in the human hair matrix and pre-cortex in relation to cell cycle activity and HF-specific epithelial cell differentiation that is marked by keratin 85 (K85) protein expression. K85 expression coincided with loss or reduction in cell cycle activity markers, including in situ DNA synthesis (EdU incorporation), Ki-67, phospho-histone H3 and cyclins A and B1, affirming a post-mitotic state of pre-cortical HF keratinocytes. Expression of CIP/KIP proteins was found abundantly within the proliferative hair matrix, concomitant with a role in cell cycle checkpoint control. p21(CIP1), p27(Klp1) and cyclin E persisted within post-mitotic keratinocytes of the pre-cortex, whereas p57(MP2) protein decreased but became nuclear. These data imply a supportive role for CIP/KIP proteins in maintaining proliferative arrest, differentiation and anti-apoptotic pathways, promoting continuous hair bulb growth and hair shaft formation in anagen VI. Moreover, post-mitotic hair matrix regions contained cells with enlarged nuclei, and DNA in situ hybridisation showed cells that w ere >2N in the pre-cortex. This suggests that CIP/KIP proteins might counterbalance cyclin E to control further rounds o f DNA replication in a cell population that has a propensity to become tetraploid. These data shed new light on the in situ-biography of human hair matrix keratinocytes on their path of active cell cycling, arrest and terminal differentiation, and showcase the human HF as an excellent, clinically relevant model system for cell cycle physiology research of human epithelial cells within their natural tissue habitat. Crown Copyright (C) 2017 Published by Elsevier GmbH. All rights reserved.
KW  - Human hair follicle
KW  - Hair matrix
KW  - Proliferation
KW  - Differentiation
KW  - Cell cycle arrest
KW  - CIP/KIP
Y1  - 2017
U6  - https://doi.org/10.1016/j.ejcb.2017.03.011
SN  - 0171-9335
SN  - 1618-1298
VL  - 96
SP  - 632
EP  - 641
PB  - Elsevier
CY  - Jena
ER  -