TY  - JOUR
A1  - Leimkühler, Silke
T1  - The biosynthesis of the molybdenum cofactors in Escherichia coli
JF  - Environmental microbiology
N2  - The biosynthesis of the molybdenum cofactor (Moco) is highly conserved among all kingdoms of life. In all molybdoenzymes containing Moco, the molybdenum atom is coordinated to a dithiolene group present in the pterin-based 6-alkyl side chain of molybdopterin (MPT). In general, the biosynthesis of Moco can be divided into four steps in in bacteria: (i) the starting point is the formation of the cyclic pyranopterin monophosphate (cPMP) from 5 '-GTP, (ii) in the second step the two sulfur atoms are inserted into cPMP leading to the formation of MPT, (iii) in the third step the molybdenum atom is inserted into MPT to form Moco and (iv) in the fourth step bis-Mo-MPT is formed and an additional modification of Moco is possible with the attachment of a nucleotide (CMP or GMP) to the phosphate group of MPT, forming the dinucleotide variants of Moco. This review presents an update on the well-characterized Moco biosynthesis in the model organism Escherichia coli including novel discoveries from the recent years.
KW  - periplasmic nitrate reductase
KW  - biotin sulfoxide reductase
KW  - in-vitro-synthesis
KW  - n-oxide reductase
KW  - crystal-structure
KW  - molybdopterin synthase
KW  - formate dehydrogenase
KW  - rhodobacter-capsulatus
KW  - xanthine dehydrogenase
KW  - converting factor
Y1  - 2020
U6  - https://doi.org/10.1111/1462-2920.15003
SN  - 1462-2912
SN  - 1462-2920
VL  - 22
IS  - 6
SP  - 2007
EP  - 2026
PB  - Wiley
CY  - Hoboken
ER  -