TY  - JOUR
A1  - Delfan, Maryam
A1  - Juybari, Raheleh Amadeh
A1  - Gorgani-Firuzjaee, Sattar
A1  - Høiriis Nielsen, Jens
A1  - Delfan, Neda
A1  - Laher, Ismail
A1  - Saeidi, Ayoub
A1  - Granacher, Urs
A1  - Zouhal, Hassane
T1  - High-intensity interval training improves cardiac function by miR-206 dependent HSP60 induction in diabetic rats
JF  - Frontiers in cardiovascular medicine
N2  - ObjectiveA role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. MethodsEighteen male Wistar rats (260 +/- 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions. ResultsBoth exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, eta(2): 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, eta(2): 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, eta(2): 0.976), with a nearly 50% greater increase in the HIIT group compared with MICT. ConclusionsOur results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.
KW  - diabetes
KW  - apoptosis
KW  - miRNAs
KW  - exercise
KW  - cardiomyopathy
Y1  - 2022
U6  - https://doi.org/10.3389/fcvm.2022.927956
SN  - 2297-055X
VL  - 9
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Delfan, Maryam
A1  - Juybari, Raheleh Amadeh
A1  - Gorgani-Firuzjaee, Sattar
A1  - Nielsen, Jens Høiriis
A1  - Delfan, Neda
A1  - Laher, Ismail
A1  - Saeidi, Ayoub
A1  - Granacher, Urs
A1  - Zouhal, Hassane
T1  - High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
JF  - Frontiers in Cardiovascular Medicine
N2  - Objective: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats.

Methods: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions.

Results: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η2: 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η2: 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η2: 0.976), with a nearly 50% greater increase in the HIIT group compared with MICT.

Conclusions: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.
KW  - diabetes
KW  - apoptosis
KW  - miRNAs
KW  - exercise
KW  - cardiomyopathy
Y1  - 2022
U6  - https://doi.org/10.3389/fcvm.2022.927956
SN  - 2297-055X
VL  - 9
SP  - 1
EP  - 11
PB  - Frontiers
CY  - Lausanne, Schweiz
ER  -