TY  - JOUR
A1  - Pinyou, Piyanut
A1  - Ruff, Adrian
A1  - Poeller, Sascha
A1  - Alsaoub, Sabine
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Schuhmann, Wolfgang
T1  - Wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces via entrapment in low potential phenothiazine-modified redox polymers
JF  - Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society
N2  - Phenothiazine-modified redox hydrogels were synthesized and used for the wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces. The effects of the pH value and electrode surface modification on the biocatalytic activity of the layers were studied in the presence of vanillin as the substrate. The enzyme electrodes were successfully employed as bioanodes in vanillin/O-2 biofuel cells in combination with a high potential bilirubin oxidase biocathode. Open circuit voltages of around 700 mV could be obtained in a two compartment biofuel cell setup. Moreover, the use of a rather hydrophobic polymer with a high degree of crosslinking sites ensures the formation of stable polymer/enzyme films which were successfully used as bioanode in membrane-less biofuel cells. (C) 2015 Elsevier B.V. All rights reserved.
KW  - Aldehyde oxidoreductase
KW  - Enzyme electrode
KW  - Redox polymer
KW  - Phenothiazine
KW  - Biosensor
KW  - Biofuel cell
Y1  - 2016
U6  - https://doi.org/10.1016/j.bioelechem.2015.12.005
SN  - 1567-5394
SN  - 1878-562X
VL  - 109
SP  - 24
EP  - 30
PB  - Elsevier
CY  - Lausanne
ER  -