TY  - JOUR
A1  - Gietler, Marta
A1  - Nykiel, Malgorzata
A1  - Orzechowski, Slawomir
A1  - Zagdanska, Barbara
A1  - Fettke, Jörg
T1  - Proteomic analysis of S-nitrosylated and S-glutathionylated proteins in wheat seedlings with different dehydration tolerances
JF  - Plant physiology and biochemistry : an official journal of the Federation of European Societies of Plant Physiology
N2  - A loss of dehydration tolerance in wheat seedlings on the fifth day following imbibition is associated with a disturbance in cellular redox homeostasis, as documented by a shift of the reduced/oxidized glutathione ratio to a more oxidized state and a significant increase in the ratio of protein thiols to the total thiol group content. Therefore, the identification and characterization of redox-sensitive proteins are important steps toward understanding the molecular mechanisms of the loss of dehydration tolerance. In the present study, proteins that were differentially expressed between fully turgid (control), dehydrated tolerant (four-day-old) and dehydrated sensitive (six-day-old) wheat seedlings were analysed. Protein spots having at least a significant (p < 0.05) two-fold change in protein abundance were selected by Delta2D as differentially expressed, identified by MALDI-TOF and LC-MS/MS, and classified according to their function. The observed changes in the proteomic patterns of the differentially S-nitrosylated and S-glutathionylated proteins were highly specific in dehydration-tolerant and-sensitive wheat seedlings. The metabolic function of these proteins indicates that dehydration tolerance is mainly related to nucleic acids, protein metabolism, and energy metabolism. It has been proven that leaf-specific thionins BTH6 and DB4, chloroplastic 50S ribosomal protein L16, phospholipase A1-II delta, and chloroplastic thioredoxin M2 are both S-nitrosylated and S-glutathionylated upon water deficiency. Our results revealed the existence of interplay between S-nitrosylation and S-glutathionylation, two redox-regulated protein posttranslational modifications that could enhance plant defence mechanisms and/or facilitate the acclimation of plants to unfavourable environmental conditions. (C) 2016 Elsevier Masson SAS. All rights reserved.
KW  - Dehydration tolerance
KW  - Proteomics
KW  - Redox sensitive proteins
KW  - S-glutathionylation
KW  - S-nitrosylation
KW  - Triticum aestivum L.
Y1  - 2016
U6  - https://doi.org/10.1016/j.plaphy.2016.08.017
SN  - 0981-9428
VL  - 108
SP  - 507
EP  - 518
PB  - Elsevier
CY  - Paris
ER  -