TY  - JOUR
A1  - Li, Zhengdong
A1  - Xu, Xun
A1  - Wang, Weiwei
A1  - Kratz, Karl
A1  - Sun, Xianlei
A1  - Zou, Jie
A1  - Deng, Zijun
A1  - Jung, Friedrich Wilhelm
A1  - Gossen, Manfred
A1  - Ma, Nan
A1  - Lendlein, Andreas
T1  - Modulation of the mesenchymal stem cell migration capacity via preconditioning with topographic microstructure
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - Controlling mesenchymal stem cells (MSCs) behavior is necessary to fully exploit their therapeutic potential. Various approaches are employed to effectively influence the migration capacity of MSCs. Here, topographic microstructures with different microscale roughness were created on polystyrene (PS) culture vessel surfaces as a feasible physical preconditioning strategy to modulate MSC migration. By analyzing trajectories of cells migrating after reseeding, we demonstrated that the mobilization velocity of human adipose derived mesenchymal stem cells (hADSCs) could be promoted by and persisted after brief preconditioning with the appropriate microtopography. Moreover, the elevated activation levels of focal adhesion kinase (FAK) and mitogen-activated protein kinase (MAPK) in hADSCs were also observed during and after the preconditioning process. These findings underline the potential enhancement of in vivo therapeutic efficacy in regenerative medicine via transplantation of topographic microstructure preconditioned stem cells.
KW  - Mesenchymal stem cells
KW  - precondition
KW  - microstructure
KW  - migration
KW  - FAK-MAPK
Y1  - 2017
U6  - https://doi.org/10.3233/CH-179208
SN  - 1386-0291
SN  - 1875-8622
VL  - 67
SP  - 267
EP  - 278
PB  - IOS Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Zou, Jie
A1  - Wang, Weiwei
A1  - Neffe, Axel T.
A1  - Xu, Xun
A1  - Li, Zhengdong
A1  - Deng, Zijun
A1  - Sun, Xianlei
A1  - Ma, Nan
A1  - Lendlein, Andreas
T1  - Adipogenic differentiation of human adipose derived mesenchymal stem cells in 3D architectured gelatin based hydrogels (ArcGel)
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - Polymeric matrices mimicking multiple functions of the ECM are expected to enable a material induced regeneration of tissues. Here, we investigated the adipogenic differentiation of human adipose derived mesenchymal stem cells (hADSCs) in a 3D architectured gelatin based hydrogel (ArcGel) prepared from gelatin and L-lysine diisocyanate ethyl ester (LDI) in an one-step process, in which the formation of an open porous morphology and the chemical network formation were integrated. The ArcGel was designed to support adipose tissue regeneration with its 3D porous structure, high cell biocompatibility, and mechanical properties compatible with human subcutaneous adipose tissue. The ArcGel could support initial cell adhesion and survival of hADSCs. Under static culture condition, the cells could migrate into the inner part of the scaffold with a depth of 840 +/- 120 mu m after 4 days, and distributed in the whole scaffold (2mm in thickness) within 14 days. The cells proliferated in the scaffold and the fold increase of cell number after 7 days of culture was 2.55 +/- 0.08. The apoptotic rate of hADSCs in the scaffold was similar to that of cells maintained on tissue culture plates. When cultured in adipogenic induction medium, the hADSCs in the scaffold differentiated into adipocytes with a high efficiency (93 +/- 1%). Conclusively, this gelatin based 3D scaffold presented high cell compatibility for hADSC cultivation and differentiation, which could serve as a potential implant material in clinical applications for adipose tissue reparation and regeneration.
KW  - Mesenchymal stem cells
KW  - gelatin based scaffold
KW  - adipose tissue regeneration
KW  - adipogenic differentiation
Y1  - 2017
U6  - https://doi.org/10.3233/CH-179210
SN  - 1386-0291
SN  - 1875-8622
VL  - 67
IS  - 3-4
SP  - 297
EP  - 307
PB  - IOS Press
CY  - Amsterdam
ER  -