TY  - JOUR
A1  - Kasyanenko, Nina
A1  - Lysyakova, Liudmila
A1  - Ramazanov, Ruslan
A1  - Nesterenko, Alexey
A1  - Yaroshevich, Igor
A1  - Titov, Evgenii
A1  - Alexeev, G.
A1  - Lezov, Andrey
A1  - Unksov, I.
T1  - Conformational and Phase Transitions in DNA-Photosensitive Surfactant Solutions: Experiment and Modeling
JF  - Biopolymers
N2  - DNA binding to trans- and cis-isomers of azobenzene containing cationic surfactant in 5 mM NaCl solution was investigated by the methods of dynamic light scattering (DLS), low-gradient viscometry (LGV), atomic force microscopy (AFM), circular dichroism (CD), gel electrophoresis (GE), flow birefringence (FB), UV-Vis spectrophotometry. Light-responsive conformational transitions of DNA in complex with photosensitive surfactant, changes in DNA optical anisotropy and persistent length, phase transition of DNA into nanoparticles induced by high surfactant concentration, as well as transformation of surfactant conformation under its binding to macromolecule were studied. Computer simulations of micelles formation for cis- and trans-isomers of azobenzene containing surfactant, as well as DNA-surfactant interaction, were carried out. Phase diagram for DNA-surfactant solutions was designed. The possibility to reverse the DNA packaging induced by surfactant binding with the dilution and light irradiation was shown. (c) 2014 Wiley Periodicals, Inc. Biopolymers 103: 109-122, 2015.
KW  - DNA-surfactant complexes
KW  - light-induced DNA de-compaction
KW  - phase diagram
KW  - DNA volume and persistent length
Y1  - 2015
U6  - https://doi.org/10.1002/bip.22575
SN  - 0006-3525
SN  - 1097-0282
VL  - 103
IS  - 2
SP  - 109
EP  - 122
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -